The Cyclic AMP Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells

Pietro Marini, Maria Grazia Cascio, Roger G. Pertwee

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

The cyclic AMP assay is a functional assay that is commonly used to determine the pharmacological behavior (agonists, antagonists, inverse agonists) of G-protein-coupled receptor (GPCR) ligands. Here, we describe the cyclic AMP assay that is carried out with commercially available non-radioligand ready-to-use kits and Chinese hamster ovarian (CHO) cells stably transfected with the human cannabinoid CB2 receptor.
Original languageEnglish
Title of host publicationEndocannabinoid Signaling
Subtitle of host publicationMethods and Protocols
EditorsMauro Maccarrone
Place of PublicationNew York
PublisherSpringer Science+Business Media
Pages85-93
Number of pages9
ISBN (Electronic)9781493935390
ISBN (Print)9781493935376
DOIs
Publication statusPublished - 2016

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume1412
ISSN (Print)1064-3745

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Keywords

  • cyclic AMP
  • cannabinoid receptor type 2
  • Forskolin (FSK)
  • agonist
  • antagonist
  • inverse agonist
  • 3-isobutyl-1-methylxanthine

Cite this

Marini, P., Cascio, M. G., & Pertwee, R. G. (2016). The Cyclic AMP Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells. In M. Maccarrone (Ed.), Endocannabinoid Signaling: Methods and Protocols (pp. 85-93). (Methods in Molecular Biology; Vol. 1412). New York: Springer Science+Business Media. https://doi.org/10.1007/978-1-4939-3539-0_9