The Cyclic AMP Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells

Pietro Marini, Maria Grazia Cascio, Roger G. Pertwee

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

The cyclic AMP assay is a functional assay that is commonly used to determine the pharmacological behavior (agonists, antagonists, inverse agonists) of G-protein-coupled receptor (GPCR) ligands. Here, we describe the cyclic AMP assay that is carried out with commercially available non-radioligand ready-to-use kits and Chinese hamster ovarian (CHO) cells stably transfected with the human cannabinoid CB2 receptor.
Original languageEnglish
Title of host publicationEndocannabinoid Signaling
Subtitle of host publicationMethods and Protocols
EditorsMauro Maccarrone
Place of PublicationNew York
PublisherSpringer Science+Business Media
Pages85-93
Number of pages9
ISBN (Electronic)9781493935390
ISBN (Print)9781493935376
DOIs
Publication statusPublished - 2016

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume1412
ISSN (Print)1064-3745

Fingerprint

Cannabinoid Receptor CB2
Cyclic AMP
G-Protein-Coupled Receptors
Cricetulus
Pharmacology
Ligands

Keywords

  • cyclic AMP
  • cannabinoid receptor type 2
  • Forskolin (FSK)
  • agonist
  • antagonist
  • inverse agonist
  • 3-isobutyl-1-methylxanthine

Cite this

Marini, P., Cascio, M. G., & Pertwee, R. G. (2016). The Cyclic AMP Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells. In M. Maccarrone (Ed.), Endocannabinoid Signaling: Methods and Protocols (pp. 85-93). (Methods in Molecular Biology; Vol. 1412). New York: Springer Science+Business Media. https://doi.org/10.1007/978-1-4939-3539-0_9

The Cyclic AMP Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells. / Marini, Pietro; Cascio, Maria Grazia; Pertwee, Roger G.

Endocannabinoid Signaling: Methods and Protocols. ed. / Mauro Maccarrone. New York : Springer Science+Business Media, 2016. p. 85-93 (Methods in Molecular Biology; Vol. 1412).

Research output: Chapter in Book/Report/Conference proceedingChapter

Marini, P, Cascio, MG & Pertwee, RG 2016, The Cyclic AMP Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells. in M Maccarrone (ed.), Endocannabinoid Signaling: Methods and Protocols. Methods in Molecular Biology, vol. 1412, Springer Science+Business Media, New York, pp. 85-93. https://doi.org/10.1007/978-1-4939-3539-0_9
Marini P, Cascio MG, Pertwee RG. The Cyclic AMP Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells. In Maccarrone M, editor, Endocannabinoid Signaling: Methods and Protocols. New York: Springer Science+Business Media. 2016. p. 85-93. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-3539-0_9
Marini, Pietro ; Cascio, Maria Grazia ; Pertwee, Roger G. / The Cyclic AMP Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells. Endocannabinoid Signaling: Methods and Protocols. editor / Mauro Maccarrone. New York : Springer Science+Business Media, 2016. pp. 85-93 (Methods in Molecular Biology).
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