The cyclopentenone prostaglandin 15-deoxy-Delta(12,14)-prostaglandin J2 ameliorates ischemic acute renal failure

P K Chatterjee, N S A Patel, S Cuzzocrea, P A J Brown, Keith Nicol Stewart, H Mota-Filipe, D Britti, W Eberhardt, J Pfeilschifter, C Thiemermann

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Abstract

Objective: Here we investigate the effects of the endogenous prostaglandin D-2 metabolite 15-deoxy-Delta(12,14)-prostaglandin J(2), on the renal dysfunction and injury caused by ischemia/reperfusion of the kidney. Methods: Male Wistar rats, subjected to bilateral renal ischemia for 45 min followed by reperfusion for up to 48 h, were administered 15-deoxy-Delta(12,14)-prostaglandin J(2) (1 mg/kg, intravenously) 5 min prior to and again after 3 or 12 h reperfusion. Results: 15-deoxy-Delta(12,14)-prostaglandin J(2) significantly reduced (i) renal and tubular dysfunction (serum urea and creatinine levels, creatinine clearance, fractional excretion of Na+ (FENa)), (ii) tubular and reperfusion-injury (urinary N-acetyl-beta-D-glucosaminidase, aspartate aminotransferase (ASP) and gamma-glutamyltransferase (gamma-GT)) and (iii) histological evidence of renal injury. 15-deoxy-Delta(12,14)-prostaglandin J(2) also improved renal function (plasma creatinine levels) and reduced the histological signs of renal injury (after 48 h reperfusion). Administration of 15-deoxy-Delta(12,14)-prostaglandin J, markedly reduced the expression of inducible nitric oxide synthase (NOS) and intercellular adhesion molecule-1 during reperfusion (determined using immunohistochemistry). Immunohistochemical analysis of p65 translocation and Western blot analysis Of IkappaB-alpha degradation revealed that 15-deoxy-Delta(12,14)-prostaglandin J(2) inhibited the activation of nuclear factor (NF)-kappaB in renal cells. Subsequently, 15d-PGJ(2) was able to significantly reduce nitric oxide production during renal ischemia/reperfusion and by primary cultures of rat proximal tubular (PT) cells incubated with interferon-gamma and bacterial lipopolysaccharide (LPS) in combination. Conclusions: We demonstrate here, for the first time, that 15-deoxy-Delta(12,14)-prostaglandin J(2) significantly reduces renal ischemia/reperfusion-injury via reduction of pro-inflammatory gene expression during reperfusion subsequent to the inhibition of the activation of NF-kappaB. (C) 2003 European Society of Cardiology. Published by Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)630-643
Number of pages14
JournalCardiovascular Research
Volume61
Issue number3
DOIs
Publication statusPublished - 15 Feb 2004

Keywords

  • renal function
  • ischemia
  • reperfusion
  • prostaglandins
  • rat
  • activated-receptor-gamma
  • NF-kappa-B
  • PPAR-gamma
  • nitric oxide
  • mesangial cells
  • reperfusion injury
  • chronic inflammation
  • in-vivo
  • expression

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  • Cite this

    Chatterjee, P. K., Patel, N. S. A., Cuzzocrea, S., Brown, P. A. J., Stewart, K. N., Mota-Filipe, H., Britti, D., Eberhardt, W., Pfeilschifter, J., & Thiemermann, C. (2004). The cyclopentenone prostaglandin 15-deoxy-Delta(12,14)-prostaglandin J2 ameliorates ischemic acute renal failure. Cardiovascular Research, 61(3), 630-643. https://doi.org/10.1016/j.cardiores.2003.10.024