The detection of Cryptosporidium parvum and Escherichia coli O157 in UK bivalve shellfish

Marion MacRae, Norval James Colin Strachan, S. Wright, Iain D Ogden

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Optimised immunomagnetic separation methods to detect Cryptosporidium parvum and Escherichia coli O157 in UK shellfish are described. Whole tissue homogenates gave the best recoveries for C. parvum oocysts compared with gill or haemolymph extracts. The sensitivity of recovery from spiked samples was comparable to that achieved when processing water and varied from 12-34% in mussels, 48-69.5% in oysters and 30-65% in scallops. Maximum recovery of E. coli O157 was achieved by enriching in buffered peptone water supplemented with vancomycin at 42 degreesC. Increasing enrichment temperatures from 37 to 42 degreesC gave a significant increase in target number recovery. Implementation of these methods into monitoring programmes and end-product testing will enable shellfish producers to better assess product safety. (C) 2004 Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)395-401
Number of pages6
JournalJournal of Microbiological Methods
Volume60
DOIs
Publication statusPublished - 2005

Keywords

  • C. parvum
  • E. coli O157
  • shellfish
  • immunomagnetic separation
  • IMMUNOMAGNETIC SEPARATION
  • INFECTION
  • OUTBREAK
  • OOCYSTS
  • CONSUMPTION

Cite this

The detection of Cryptosporidium parvum and Escherichia coli O157 in UK bivalve shellfish. / MacRae, Marion; Strachan, Norval James Colin; Wright, S.; Ogden, Iain D.

In: Journal of Microbiological Methods, Vol. 60, 2005, p. 395-401.

Research output: Contribution to journalArticle

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