Abstract
Optimised immunomagnetic separation methods to detect Cryptosporidium parvum and Escherichia coli O157 in UK shellfish are described. Whole tissue homogenates gave the best recoveries for C. parvum oocysts compared with gill or haemolymph extracts. The sensitivity of recovery from spiked samples was comparable to that achieved when processing water and varied from 12-34% in mussels, 48-69.5% in oysters and 30-65% in scallops. Maximum recovery of E. coli O157 was achieved by enriching in buffered peptone water supplemented with vancomycin at 42 degreesC. Increasing enrichment temperatures from 37 to 42 degreesC gave a significant increase in target number recovery. Implementation of these methods into monitoring programmes and end-product testing will enable shellfish producers to better assess product safety. (C) 2004 Elsevier B.V. All rights reserved.
| Original language | English |
|---|---|
| Pages (from-to) | 395-401 |
| Number of pages | 6 |
| Journal | Journal of Microbiological Methods |
| Volume | 60 |
| DOIs | |
| Publication status | Published - 2005 |
Keywords
- C. parvum
- E. coli O157
- shellfish
- immunomagnetic separation
- IMMUNOMAGNETIC SEPARATION
- INFECTION
- OUTBREAK
- OOCYSTS
- CONSUMPTION
Cite this
The detection of Cryptosporidium parvum and Escherichia coli O157 in UK bivalve shellfish. / MacRae, Marion; Strachan, Norval James Colin; Wright, S.; Ogden, Iain D.
In: Journal of Microbiological Methods, Vol. 60, 2005, p. 395-401.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The detection of Cryptosporidium parvum and Escherichia coli O157 in UK bivalve shellfish
AU - MacRae, Marion
AU - Strachan, Norval James Colin
AU - Wright, S.
AU - Ogden, Iain D
PY - 2005
Y1 - 2005
N2 - Optimised immunomagnetic separation methods to detect Cryptosporidium parvum and Escherichia coli O157 in UK shellfish are described. Whole tissue homogenates gave the best recoveries for C. parvum oocysts compared with gill or haemolymph extracts. The sensitivity of recovery from spiked samples was comparable to that achieved when processing water and varied from 12-34% in mussels, 48-69.5% in oysters and 30-65% in scallops. Maximum recovery of E. coli O157 was achieved by enriching in buffered peptone water supplemented with vancomycin at 42 degreesC. Increasing enrichment temperatures from 37 to 42 degreesC gave a significant increase in target number recovery. Implementation of these methods into monitoring programmes and end-product testing will enable shellfish producers to better assess product safety. (C) 2004 Elsevier B.V. All rights reserved.
AB - Optimised immunomagnetic separation methods to detect Cryptosporidium parvum and Escherichia coli O157 in UK shellfish are described. Whole tissue homogenates gave the best recoveries for C. parvum oocysts compared with gill or haemolymph extracts. The sensitivity of recovery from spiked samples was comparable to that achieved when processing water and varied from 12-34% in mussels, 48-69.5% in oysters and 30-65% in scallops. Maximum recovery of E. coli O157 was achieved by enriching in buffered peptone water supplemented with vancomycin at 42 degreesC. Increasing enrichment temperatures from 37 to 42 degreesC gave a significant increase in target number recovery. Implementation of these methods into monitoring programmes and end-product testing will enable shellfish producers to better assess product safety. (C) 2004 Elsevier B.V. All rights reserved.
KW - C. parvum
KW - E. coli O157
KW - shellfish
KW - immunomagnetic separation
KW - IMMUNOMAGNETIC SEPARATION
KW - INFECTION
KW - OUTBREAK
KW - OOCYSTS
KW - CONSUMPTION
U2 - 10.1016/j.mimet.2004.10.017
DO - 10.1016/j.mimet.2004.10.017
M3 - Article
VL - 60
SP - 395
EP - 401
JO - Journal of Microbiological Methods
JF - Journal of Microbiological Methods
SN - 0167-7012
ER -