The differential regulation of CART gene expression in a pituitary cell line and primary cell cultures of ovine pars tuberalis cells

Perry Barrett, Amanda Morris, Kim-Marie Moar, Julian Mercer, J A Davidson, P.A. Findlay, Clare Lesley Adam, Peter John Morgan

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

The cocaine-amphetamine regulated transcript (CART) encodes for a protein which has an important role in the regulation of appetite and body weight. To date, no details of the molecular events and signal transduction pathways which regulate this gene are available. We report the identification of CART gene expression in the GH3 pituitary cell line. We have used activators of the cAMP or protein kinase C (PKC) signal transduction pathways to show that, in GH3 cells, CART is transcriptionally up-regulated by activators of the cAMP signal transduction pathway. We also identify CART gene expression in ovine pars tuberalis (PT) tissue and primary cell cultures. In PT cells in contrast to GH3 cells, CART gene expression is upregulated by activators of the PKC signal transduction pathway. Cultured cells have provided a valuable resource for the detailed analysis of specific regulatory mechanisms underlying transcriptional or translational regulation of genes, signal transduction events and many other cellular processes. GH3 and PT cells may therefore provide a resource for the further detailed molecular analysis of the events regulating CART gene expression and processing.

Original languageEnglish
Pages (from-to)347-352
Number of pages6
JournalJournal of Neuroendocrinology
Volume13
Issue number4
DOIs
Publication statusPublished - Apr 2001

Keywords

  • hypothalamus
  • neuropeptide
  • obesity
  • appetite
  • pituitary
  • thyroprotein-releasing-hormone
  • adenylate-cyclase
  • proopielanocortin gene
  • messenger RNA
  • transcription
  • amphetamine
  • melatonin
  • mechanism
  • cocaine
  • thyrotropin-releasing hormone
  • proopiomelanocortin gene
  • messenger-RNA

Cite this

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title = "The differential regulation of CART gene expression in a pituitary cell line and primary cell cultures of ovine pars tuberalis cells",
abstract = "The cocaine-amphetamine regulated transcript (CART) encodes for a protein which has an important role in the regulation of appetite and body weight. To date, no details of the molecular events and signal transduction pathways which regulate this gene are available. We report the identification of CART gene expression in the GH3 pituitary cell line. We have used activators of the cAMP or protein kinase C (PKC) signal transduction pathways to show that, in GH3 cells, CART is transcriptionally up-regulated by activators of the cAMP signal transduction pathway. We also identify CART gene expression in ovine pars tuberalis (PT) tissue and primary cell cultures. In PT cells in contrast to GH3 cells, CART gene expression is upregulated by activators of the PKC signal transduction pathway. Cultured cells have provided a valuable resource for the detailed analysis of specific regulatory mechanisms underlying transcriptional or translational regulation of genes, signal transduction events and many other cellular processes. GH3 and PT cells may therefore provide a resource for the further detailed molecular analysis of the events regulating CART gene expression and processing.",
keywords = "hypothalamus, neuropeptide, obesity, appetite, pituitary, thyroprotein-releasing-hormone, adenylate-cyclase, proopielanocortin gene, messenger RNA, transcription, amphetamine, melatonin, mechanism, cocaine, thyrotropin-releasing hormone, proopiomelanocortin gene, messenger-RNA",
author = "Perry Barrett and Amanda Morris and Kim-Marie Moar and Julian Mercer and Davidson, {J A} and P.A. Findlay and Adam, {Clare Lesley} and Morgan, {Peter John}",
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T1 - The differential regulation of CART gene expression in a pituitary cell line and primary cell cultures of ovine pars tuberalis cells

AU - Barrett, Perry

AU - Morris, Amanda

AU - Moar, Kim-Marie

AU - Mercer, Julian

AU - Davidson, J A

AU - Findlay, P.A.

AU - Adam, Clare Lesley

AU - Morgan, Peter John

PY - 2001/4

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N2 - The cocaine-amphetamine regulated transcript (CART) encodes for a protein which has an important role in the regulation of appetite and body weight. To date, no details of the molecular events and signal transduction pathways which regulate this gene are available. We report the identification of CART gene expression in the GH3 pituitary cell line. We have used activators of the cAMP or protein kinase C (PKC) signal transduction pathways to show that, in GH3 cells, CART is transcriptionally up-regulated by activators of the cAMP signal transduction pathway. We also identify CART gene expression in ovine pars tuberalis (PT) tissue and primary cell cultures. In PT cells in contrast to GH3 cells, CART gene expression is upregulated by activators of the PKC signal transduction pathway. Cultured cells have provided a valuable resource for the detailed analysis of specific regulatory mechanisms underlying transcriptional or translational regulation of genes, signal transduction events and many other cellular processes. GH3 and PT cells may therefore provide a resource for the further detailed molecular analysis of the events regulating CART gene expression and processing.

AB - The cocaine-amphetamine regulated transcript (CART) encodes for a protein which has an important role in the regulation of appetite and body weight. To date, no details of the molecular events and signal transduction pathways which regulate this gene are available. We report the identification of CART gene expression in the GH3 pituitary cell line. We have used activators of the cAMP or protein kinase C (PKC) signal transduction pathways to show that, in GH3 cells, CART is transcriptionally up-regulated by activators of the cAMP signal transduction pathway. We also identify CART gene expression in ovine pars tuberalis (PT) tissue and primary cell cultures. In PT cells in contrast to GH3 cells, CART gene expression is upregulated by activators of the PKC signal transduction pathway. Cultured cells have provided a valuable resource for the detailed analysis of specific regulatory mechanisms underlying transcriptional or translational regulation of genes, signal transduction events and many other cellular processes. GH3 and PT cells may therefore provide a resource for the further detailed molecular analysis of the events regulating CART gene expression and processing.

KW - hypothalamus

KW - neuropeptide

KW - obesity

KW - appetite

KW - pituitary

KW - thyroprotein-releasing-hormone

KW - adenylate-cyclase

KW - proopielanocortin gene

KW - messenger RNA

KW - transcription

KW - amphetamine

KW - melatonin

KW - mechanism

KW - cocaine

KW - thyrotropin-releasing hormone

KW - proopiomelanocortin gene

KW - messenger-RNA

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DO - 10.1046/j.1365-2826.2001.00634.x

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