The Displacement Binding Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells

Maria Grazia Cascio, Pietro Marini, Roger G. Pertwee

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Displacement binding assays are nonfunctional assays mostly used with the aim of determining whether a certain compound (plant-derived or synthetic) is able to bind to a specific receptor with high affinity. Here, we describe the displacement binding assay that is carried out with a radioligand and CHO (Chinese Hamster Ovarian) cells stably transfected with the human cannabinoid CB2 receptor.
Original languageEnglish
Title of host publicationEndocannabinoid Signaling
Subtitle of host publicationMethods and Protocols
EditorsMauro Maccarrone
PublisherSpringer Science+Business Media
Pages57-63
Number of pages7
Volume1412
ISBN (Electronic)978-1-4939-3539-0
ISBN (Print)978-1-4939-3537-6
DOIs
Publication statusPublished - 1 Jun 2016

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume1412
ISSN (Print)1064-3745

Fingerprint

Cannabinoid Receptor CB2
Phytochemicals
Cricetulus

Keywords

  • binding
  • cannabinoid CB2 receptors
  • agonist
  • antagonist
  • ligand
  • radioligand
  • affinity

Cite this

Cascio, M. G., Marini, P., & Pertwee, R. G. (2016). The Displacement Binding Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells. In M. Maccarrone (Ed.), Endocannabinoid Signaling: Methods and Protocols (Vol. 1412, pp. 57-63). (Methods in Molecular Biology; Vol. 1412). Springer Science+Business Media. https://doi.org/10.1007/978-1-4939-3539-0_6

The Displacement Binding Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells. / Cascio, Maria Grazia; Marini, Pietro; Pertwee, Roger G.

Endocannabinoid Signaling: Methods and Protocols. ed. / Mauro Maccarrone. Vol. 1412 Springer Science+Business Media, 2016. p. 57-63 (Methods in Molecular Biology; Vol. 1412).

Research output: Chapter in Book/Report/Conference proceedingChapter

Cascio, MG, Marini, P & Pertwee, RG 2016, The Displacement Binding Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells. in M Maccarrone (ed.), Endocannabinoid Signaling: Methods and Protocols. vol. 1412, Methods in Molecular Biology, vol. 1412, Springer Science+Business Media, pp. 57-63. https://doi.org/10.1007/978-1-4939-3539-0_6
Cascio MG, Marini P, Pertwee RG. The Displacement Binding Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells. In Maccarrone M, editor, Endocannabinoid Signaling: Methods and Protocols. Vol. 1412. Springer Science+Business Media. 2016. p. 57-63. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-3539-0_6
Cascio, Maria Grazia ; Marini, Pietro ; Pertwee, Roger G. / The Displacement Binding Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells. Endocannabinoid Signaling: Methods and Protocols. editor / Mauro Maccarrone. Vol. 1412 Springer Science+Business Media, 2016. pp. 57-63 (Methods in Molecular Biology).
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