The Displacement Binding Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

Displacement binding assays are nonfunctional assays mostly used with the aim of determining whether a certain compound (plant-derived or synthetic) is able to bind to a specific receptor with high affinity. Here, we describe the displacement binding assay that is carried out with a radioligand and CHO (Chinese Hamster Ovarian) cells stably transfected with the human cannabinoid CB2 receptor.
Original languageEnglish
Title of host publicationEndocannabinoid Signaling
Subtitle of host publicationMethods and Protocols
EditorsMauro Maccarrone
PublisherSpringer Science+Business Media
Pages57-63
Number of pages7
Volume1412
ISBN (Electronic)978-1-4939-3539-0
ISBN (Print)978-1-4939-3537-6
DOIs
Publication statusPublished - 1 Jun 2016

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume1412
ISSN (Print)1064-3745

Keywords

  • binding
  • cannabinoid CB2 receptors
  • agonist
  • antagonist
  • ligand
  • radioligand
  • affinity

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