Displacement binding assays are nonfunctional assays mostly used with the aim of determining whether a certain compound (plant-derived or synthetic) is able to bind to a specific receptor with high affinity. Here, we describe the displacement binding assay that is carried out with a radioligand and CHO (Chinese Hamster Ovarian) cells stably transfected with the human cannabinoid CB2 receptor.
|Title of host publication||Endocannabinoid Signaling|
|Subtitle of host publication||Methods and Protocols|
|Publisher||Springer Science+Business Media|
|Number of pages||7|
|Publication status||Published - 1 Jun 2016|
|Name||Methods in Molecular Biology|
- cannabinoid CB2 receptors