The diversity of bacteria associated with natural aphid populations.

S. Haynes, A. C. Darby, T. J. Daniell, G. Webster, F. J. F. van Veen, H. C. F. Godfray, James Ivor Prosser, Alexander Douglas

Research output: Contribution to journalArticle

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Abstract

The bacterial communities of aphids were investigated by terminal restriction fragment length polymorphism and denaturing gradient gel electrophoresis analysis of 16S rRNA gene fragments generated by PCR with general eubacterial primers. By both methods, the gamma-proteobacterium Buchnera was detected in laboratory cultures of six parthenogenetic lines of the pea aphid Acyrthosiphon pisum and one line of the black bean aphid Aphis fabae, and one or more of four previously described bacterial taxa were also detected in all aphid lines except one of A. pisum. These latter bacteria, collectively known as secondary symbionts or accessory bacteria, comprised three taxa of gamma-proteobacteria (R-type [PASS], T-type [PABS], and U-type [PAUS]) and a rickettsia (S-type [PAR]). Complementary analysis of aphids from natural populations of four aphid species (A. pisum [n = 74], Amphorophora rubi [n = 109], Aphis sarothamni [n = 42], and Microlophium carnosum [n = 101]) from a single geographical location revealed Buchnera and up to three taxa of accessory bacteria, but no other bacterial taxa, in each aphid. The prevalence of accessory bacterial taxa varied significantly among aphid species but not with the sampling month (between June and August 2000). These results indicate that the accessory bacterial taxa are distributed across multiple aphid species, although with variable prevalence, and that laboratory culture does not generally result in a shift in the bacterial community in aphids. Both the transmission patterns of the accessory bacteria between individual aphids and their impact on aphid fitness are suggested to influence the prevalence of accessory bacterial taxa in natural aphid populations.

Original languageEnglish
Pages (from-to)7216-7223
Number of pages7
JournalApplied and Environmental Microbiology
Volume69
DOIs
Publication statusPublished - 2003

Keywords

  • 16S RIBOSOMAL-RNA
  • GRADIENT GEL-ELECTROPHORESIS
  • ACYRTHOSIPHON-PISUM INSECTA
  • PEA APHID
  • ENDOSYMBIOTIC BACTERIA
  • MICROBIAL ECOLOGY
  • BORNE BACTERIUM
  • SYMBIONTS
  • GENES
  • PCR

Cite this

Haynes, S., Darby, A. C., Daniell, T. J., Webster, G., van Veen, F. J. F., Godfray, H. C. F., ... Douglas, A. (2003). The diversity of bacteria associated with natural aphid populations. Applied and Environmental Microbiology, 69, 7216-7223. https://doi.org/10.1128/AEM.69.12.7216-7223.2003

The diversity of bacteria associated with natural aphid populations. / Haynes, S.; Darby, A. C.; Daniell, T. J.; Webster, G.; van Veen, F. J. F.; Godfray, H. C. F.; Prosser, James Ivor; Douglas, Alexander.

In: Applied and Environmental Microbiology, Vol. 69, 2003, p. 7216-7223.

Research output: Contribution to journalArticle

Haynes, S. ; Darby, A. C. ; Daniell, T. J. ; Webster, G. ; van Veen, F. J. F. ; Godfray, H. C. F. ; Prosser, James Ivor ; Douglas, Alexander. / The diversity of bacteria associated with natural aphid populations. In: Applied and Environmental Microbiology. 2003 ; Vol. 69. pp. 7216-7223.
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AU - Darby, A. C.

AU - Daniell, T. J.

AU - Webster, G.

AU - van Veen, F. J. F.

AU - Godfray, H. C. F.

AU - Prosser, James Ivor

AU - Douglas, Alexander

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AB - The bacterial communities of aphids were investigated by terminal restriction fragment length polymorphism and denaturing gradient gel electrophoresis analysis of 16S rRNA gene fragments generated by PCR with general eubacterial primers. By both methods, the gamma-proteobacterium Buchnera was detected in laboratory cultures of six parthenogenetic lines of the pea aphid Acyrthosiphon pisum and one line of the black bean aphid Aphis fabae, and one or more of four previously described bacterial taxa were also detected in all aphid lines except one of A. pisum. These latter bacteria, collectively known as secondary symbionts or accessory bacteria, comprised three taxa of gamma-proteobacteria (R-type [PASS], T-type [PABS], and U-type [PAUS]) and a rickettsia (S-type [PAR]). Complementary analysis of aphids from natural populations of four aphid species (A. pisum [n = 74], Amphorophora rubi [n = 109], Aphis sarothamni [n = 42], and Microlophium carnosum [n = 101]) from a single geographical location revealed Buchnera and up to three taxa of accessory bacteria, but no other bacterial taxa, in each aphid. The prevalence of accessory bacterial taxa varied significantly among aphid species but not with the sampling month (between June and August 2000). These results indicate that the accessory bacterial taxa are distributed across multiple aphid species, although with variable prevalence, and that laboratory culture does not generally result in a shift in the bacterial community in aphids. Both the transmission patterns of the accessory bacteria between individual aphids and their impact on aphid fitness are suggested to influence the prevalence of accessory bacterial taxa in natural aphid populations.

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KW - ACYRTHOSIPHON-PISUM INSECTA

KW - PEA APHID

KW - ENDOSYMBIOTIC BACTERIA

KW - MICROBIAL ECOLOGY

KW - BORNE BACTERIUM

KW - SYMBIONTS

KW - GENES

KW - PCR

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DO - 10.1128/AEM.69.12.7216-7223.2003

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JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

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