The DNA helicase Pfh1 promotes fork merging at replication termination sites to ensure genome stability

Roland Steinacher, Fekret Osman, Jacob Z. Dalgaard, Alexander Lorenz, Matthew C. Whitby

Research output: Contribution to journalArticle

45 Citations (Scopus)
6 Downloads (Pure)

Abstract

Bidirectionally moving DNA replication forks merge at termination sites composed of accidental or programmed DNA-protein barriers. If merging fails, then regions of unreplicated DNA can result in the breakage of DNA during mitosis, which in turn can give rise to genome instability. Despite its importance, little is known about the mechanisms that promote the final stages of fork merging in eukaryotes. Here we show that the Pif1 family DNA helicase Pfh1 plays a dual role in promoting replication fork termination. First, it facilitates replication past DNA-protein barriers, and second, it promotes the merging of replication forks. A failure of these processes in Pfh1-deficient cells results in aberrant chromosome segregation and heightened genome instability.
Original languageEnglish
Pages (from-to)594-602
Number of pages9
JournalGenes & Development
Volume26
Issue number6
DOIs
Publication statusPublished - 15 Mar 2012

Keywords

  • Pif1 family DNA helicase
  • replication termination
  • homologous recombination
  • replication fork barrier

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