The effect of delay in collection and processing on RNA integrity in human placenta: experiences from rural Africa

M. L. Jobarteh; S. E. Moore; C. Kennedy; L. Gambling; Harry J. McArdle

doi:10.1016/j.placenta.2013.08.016

The effect of delay in collection and processing on RNA integrity in human placenta: experiences from rural Africa

M. L. Jobarteh, S. E. Moore, C. Kennedy, L. Gambling, Harry J. McArdle

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Abstract

This paper examines the relationship between time to processing and RNA quality in placentas collected from women in a field setting in rural Gambia. Placental samples were collected from the villages and transferred to the laboratory. RNA was extracted using Trizol and integrity assessed using the RNA integrity number (RIN). Values were inversely correlated with delay in processing. Expression levels of candidate genes increased with decreasing RIN. Normalising to a housekeeper gene removed this artefact. We propose a cut-off point of 90 min from delivery, after which samples cannot be used for gene expression analysis. (C) 2013 The Authors. Published by Elsevier Ltd. All rights reserved.

Original language	English
Pages (from-to)	72-74
Number of pages	3
Journal	Placenta
Volume	35
Issue number	1
DOIs	https://doi.org/10.1016/j.placenta.2013.08.016
Publication status	Published - Jan 2014

Bibliographical note

Open Access funded by Medical Research Council
Under a Creative Commons license

The ENID Trial is funded by the UK Medical Research Council (core funding to the MRC International Nutrition Group; MC-A760-5QX00). This study was further supported by the Scottish Government (Rural and Environmental Sciences and Analytical Services). The authors are grateful to Dr Helen Hayes for technical assistance and are especially grateful to the mothers and the ENID Trial staff who made this study possible.

SEM conceived and designed the original ENID Trial. SEM and HJM conceived the sub-study on placental development. MLJ co-ordinated all the field work, supervised all laboratory processing in The Gambia, conducted all experimental work in Aberdeen, performed statistical analyses and wrote the first draft of the paper. CK and LG supervised all laboratory work in Aberdeen. All authors contributed to the final version of the paper. None of the authors had a conflict of interest to report.

Keywords

RNA integrity number (RIN)
ribonucleic acid (RNA)
placenta transferrin receptor
placental genes
iron metabolism

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10.1016/j.placenta.2013.08.016Licence: CC BY-NC-ND

The effect of delay in collection and processing on RNA integrity
This is an Open Access article distributed in accordance with the Creative Commons Attribution Non Commercial-No Derivs (CC BY-NC-ND 3.0) license, which permits others to distribute this work non-commercially provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc-nd/3.0/
Final published version, 490 KBLicence: CC BY-NC-ND

Cite this

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title = "The effect of delay in collection and processing on RNA integrity in human placenta: experiences from rural Africa",

abstract = "This paper examines the relationship between time to processing and RNA quality in placentas collected from women in a field setting in rural Gambia. Placental samples were collected from the villages and transferred to the laboratory. RNA was extracted using Trizol and integrity assessed using the RNA integrity number (RIN). Values were inversely correlated with delay in processing. Expression levels of candidate genes increased with decreasing RIN. Normalising to a housekeeper gene removed this artefact. We propose a cut-off point of 90 min from delivery, after which samples cannot be used for gene expression analysis. (C) 2013 The Authors. Published by Elsevier Ltd. All rights reserved.",

keywords = "RNA integrity number (RIN), ribonucleic acid (RNA), placenta transferrin receptor, placental genes, iron metabolism",

author = "Jobarteh, {M. L.} and Moore, {S. E.} and C. Kennedy and L. Gambling and McArdle, {Harry J.}",

note = "Open Access funded by Medical Research Council Under a Creative Commons license The ENID Trial is funded by the UK Medical Research Council (core funding to the MRC International Nutrition Group; MC-A760-5QX00). This study was further supported by the Scottish Government (Rural and Environmental Sciences and Analytical Services). The authors are grateful to Dr Helen Hayes for technical assistance and are especially grateful to the mothers and the ENID Trial staff who made this study possible. SEM conceived and designed the original ENID Trial. SEM and HJM conceived the sub-study on placental development. MLJ co-ordinated all the field work, supervised all laboratory processing in The Gambia, conducted all experimental work in Aberdeen, performed statistical analyses and wrote the first draft of the paper. CK and LG supervised all laboratory work in Aberdeen. All authors contributed to the final version of the paper. None of the authors had a conflict of interest to report.",

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AU - McArdle, Harry J.

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The effect of delay in collection and processing on RNA integrity in human placenta: experiences from rural Africa

Abstract

Bibliographical note

Keywords

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