The effect of dietary vitamin A and astaxanthin on the immunocompetence of rainbow trout

I. Thompson, G. Choubert, D. F. Houlihan, C. J. Secombes*

*Corresponding author for this work

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

Rainbow trout (Oncorhynchus mykiss) were maintained for 4 months on diets formulated with supplementary vitamin A (18 mg/kg dry diet) and astaxanthin (100 mg/kg dry diet) (diet A+Ax+), astaxanthin alone (diet A- Ax+), vitamin A alone (diet A+Ax-) and neither vitamin A nor astaxanthin (diet A- Ax-) to investigate the immunomodulatory effects of these compounds in fish. Serum concentrations of both vitamin A and astaxanthin reflected dietary levels, with vitamin A supplementation resulting in levels of 32-40 vs. 4 μg/dl in fish fed unsupplemented diets and astaxanthin supplementation resulting in levels of 9-11 μg/ml with levels being undetectable in fish fed unsupplemented diets. However, there was no marked effect of diet on group food conversion efficiencies or growth rates. Of the immune parameters measured, total serum antiprotease activity was found to be significantly lower in the group fed diet A-Ax- and classical serum complement activity was also lowest in this group. In addition, a trend for decreased leucocyte migration was observed in the fish fed diets A-Ax+ and A-Ax- compared with those fed diets A+Ax+ and A+A-. On the other hand, total serum immunoglobulin level, specific immunoglobulin level following immunization with Aeromonas salmonicida, serum lysozyme activity and phagocyte respiratory burst activity were unnaffected by dietary vitamin A and/or astaxanthin intake. Overall, the small immunomodulatory effect of vitamin A and/or astaxanthin supplementation observed in this study suggests that these compounds have only limited potential as immunostimulatory agents in practical rainbow trout diets.

Original languageEnglish
Pages (from-to)91-102
Number of pages12
JournalAquaculture
Volume133
Issue number2
DOIs
Publication statusPublished - Jun 1995

Fingerprint

immunocompetence
astaxanthin
vitamin
rainbow
vitamin A
Oncorhynchus mykiss
diet
serum
fish feeds
fish
effect
immunoglobulins
Aeromonas salmonicida
immunization
phagocytes
food conversion
lysozyme

Keywords

  • Astaxanthin
  • Carotenoids
  • Immunology
  • Oncorhynchus mykiss
  • Vitamins

ASJC Scopus subject areas

  • Aquatic Science

Cite this

The effect of dietary vitamin A and astaxanthin on the immunocompetence of rainbow trout. / Thompson, I.; Choubert, G.; Houlihan, D. F.; Secombes, C. J.

In: Aquaculture, Vol. 133, No. 2, 06.1995, p. 91-102.

Research output: Contribution to journalArticle

Thompson, I. ; Choubert, G. ; Houlihan, D. F. ; Secombes, C. J. / The effect of dietary vitamin A and astaxanthin on the immunocompetence of rainbow trout. In: Aquaculture. 1995 ; Vol. 133, No. 2. pp. 91-102.
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