The effect of eicosapentaenoic and docosahexaenoic acid on protein synthesis and breakdown in murine C2C12 myotubes

Torkamol Kamolrat, Stuart R. Gray

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Eicosapentaenoic acid (EPA) and Docosahexaenoic acid (DHA) have been found to stimulate protein synthesis with little information regarding the effects on protein breakdown. Furthermore whether there are distinct effects of EPA and DHA remains to be established. The aim of the current study was to determine the distinct effects of EPA and DHA on protein synthesis, protein breakdown and signalling pathways in C2C12 myotubes. Fully differentiated C2C12 cells were incubated for 24 hours with 0.1% ethanol (control), 50µM EPA or 50µM DHA prior to experimentation. After serum (4h) and amino acid (1h) starvation cells were stimulated with 2mM L-leucine and protein synthesis measured using (3)H -labelled phenylalanine. Protein breakdown was measured using (3)H-labelled phenylalanine and signalling pathways (Akt, mTOR, p70S6k, 4EBP1, rps6 and FOXO3a,) via western blots. Data revealed that after incubation with EPA protein synthesis was 25% greater (P
Original languageEnglish
Pages (from-to)593-598
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume432
Issue number4
Early online date21 Feb 2013
DOIs
Publication statusPublished - 22 Mar 2013

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Eicosapentaenoic Acid
Docosahexaenoic Acids
Skeletal Muscle Fibers
Proteins
Phenylalanine
70-kDa Ribosomal Protein S6 Kinases
Starvation
Leucine
Ethanol
Western Blotting
Amino Acids
Serum

Keywords

  • muscle
  • protein
  • fatty acids
  • fish oil
  • hypertrophy
  • atrophy

Cite this

The effect of eicosapentaenoic and docosahexaenoic acid on protein synthesis and breakdown in murine C2C12 myotubes. / Kamolrat, Torkamol; Gray, Stuart R.

In: Biochemical and Biophysical Research Communications, Vol. 432, No. 4, 22.03.2013, p. 593-598.

Research output: Contribution to journalArticle

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