The effect of exercise induced cytokines on insulin stimulated glucose transport in C2C12 cells

Stuart Robert Gray, Torkamol Kamolrat

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Abstract

Skeletal muscle contractile activity increases the production of the myokines interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-15 (IL-15) and also skeletal muscle glucose transport. Previous work has revealed a role for IL-6 in mediating glucose uptake, while research on the physiological roles of IL-8 and IL-15 is not so abundant. In the present study we investigated the effects of different concentrations and combinations of IL-6, IL-8 and IL-15 on insulin stimulated glucose transport in C2C12 cells. Furthermore, we also measured AMPK Thr172 and Akt Ser473 phosphorylation via Western blotting. Exposure to 20pg/ml of individual cytokines had no affect on glucose transport while 1ng/ml enhanced (P
Original languageEnglish
Pages (from-to)221-228
Number of pages8
JournalCytokine
Volume55
Issue number2
Early online date19 May 2011
DOIs
Publication statusPublished - Aug 2011

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Keywords

  • cytokines
  • glucose uptake
  • insulin sensitivity

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