The effect of feeding structured triacylglycerols enriched in EPA or DHA on murine splenocyte fatty acid composition and leucocyte phagocytosis

S. Kew, E. S. Gibbons, Frank Thies, G. Mcneill, P. T. Quinlan, P. C. Calder

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The effects of altering the type of n-3 polyunsaturated fatty acid (PUFA) in the mouse diet on the ability of monocytes and neutrophils to perform phagocytosis were investigated. Male weanling mice were fed for 7 d on one of nine diets which contained 178 g lipid/kg and which differed in the type of n-3 PUFA and in the position of these in dietary triacylglycerol (TAG). The control diet contained 4-4 g a-linolenic acid/100g total fatty acids. In the other diets, eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA) replaced a proportion (50 or 100 %) of the a-linolenic acid, and were in the sn-2 or the sn-1(3) position of dietary TAG. There were significant increases in the content of n-3 PUFA in spleen-cell phospholipids when EPA or DHA was fed. These increases were largely independent of the position of EPA or DHA in dietary TAG except when EPA was fed at the highest level, when the incorporation was greater when it was fed in the sn-2 than in the sn-1(3) position. There was no significant effect of dietary DHA on monocyte or neutrophil phagocytic activity. Dietary EPA dose-dependently decreased the number of monocytes and neutrophils performing phagocytosis. However, when EPA was fed in the sn-2 position, the ability of active monocytes or neutrophils to engulf bacteria was increased in a dose-dependent fashion. This did not occur when EPA was fed in the sn-1(3) position. Thus, there appears to be an influence of the position of EPA, but not of DHA, in dietary TAG on its incorporation into cell phospholipids and on the activity of phagocytic cells.

Original languageEnglish
Pages (from-to)1071-1080
Number of pages9
JournalBritish Journal of Nutrition
Volume90
DOIs
Publication statusPublished - 2003

Keywords

  • polyunsaturated fatty acids
  • fish oil
  • phagocytosis
  • immune function
  • monocytes
  • LONG-CHAIN N-3
  • KILLER-CELL ACTIVITY
  • SPRAGUE-DAWLEY RATS
  • FISH-OIL
  • DIETARY SUPPLEMENTATION
  • LYMPHOCYTE FUNCTIONS
  • MACROPHAGES
  • LIPIDS
  • PROLIFERATION
  • MANIPULATION

Cite this

The effect of feeding structured triacylglycerols enriched in EPA or DHA on murine splenocyte fatty acid composition and leucocyte phagocytosis. / Kew, S.; Gibbons, E. S.; Thies, Frank; Mcneill, G.; Quinlan, P. T.; Calder, P. C.

In: British Journal of Nutrition, Vol. 90, 2003, p. 1071-1080.

Research output: Contribution to journalArticle

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abstract = "The effects of altering the type of n-3 polyunsaturated fatty acid (PUFA) in the mouse diet on the ability of monocytes and neutrophils to perform phagocytosis were investigated. Male weanling mice were fed for 7 d on one of nine diets which contained 178 g lipid/kg and which differed in the type of n-3 PUFA and in the position of these in dietary triacylglycerol (TAG). The control diet contained 4-4 g a-linolenic acid/100g total fatty acids. In the other diets, eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA) replaced a proportion (50 or 100 {\%}) of the a-linolenic acid, and were in the sn-2 or the sn-1(3) position of dietary TAG. There were significant increases in the content of n-3 PUFA in spleen-cell phospholipids when EPA or DHA was fed. These increases were largely independent of the position of EPA or DHA in dietary TAG except when EPA was fed at the highest level, when the incorporation was greater when it was fed in the sn-2 than in the sn-1(3) position. There was no significant effect of dietary DHA on monocyte or neutrophil phagocytic activity. Dietary EPA dose-dependently decreased the number of monocytes and neutrophils performing phagocytosis. However, when EPA was fed in the sn-2 position, the ability of active monocytes or neutrophils to engulf bacteria was increased in a dose-dependent fashion. This did not occur when EPA was fed in the sn-1(3) position. Thus, there appears to be an influence of the position of EPA, but not of DHA, in dietary TAG on its incorporation into cell phospholipids and on the activity of phagocytic cells.",
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T1 - The effect of feeding structured triacylglycerols enriched in EPA or DHA on murine splenocyte fatty acid composition and leucocyte phagocytosis

AU - Kew, S.

AU - Gibbons, E. S.

AU - Thies, Frank

AU - Mcneill, G.

AU - Quinlan, P. T.

AU - Calder, P. C.

PY - 2003

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N2 - The effects of altering the type of n-3 polyunsaturated fatty acid (PUFA) in the mouse diet on the ability of monocytes and neutrophils to perform phagocytosis were investigated. Male weanling mice were fed for 7 d on one of nine diets which contained 178 g lipid/kg and which differed in the type of n-3 PUFA and in the position of these in dietary triacylglycerol (TAG). The control diet contained 4-4 g a-linolenic acid/100g total fatty acids. In the other diets, eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA) replaced a proportion (50 or 100 %) of the a-linolenic acid, and were in the sn-2 or the sn-1(3) position of dietary TAG. There were significant increases in the content of n-3 PUFA in spleen-cell phospholipids when EPA or DHA was fed. These increases were largely independent of the position of EPA or DHA in dietary TAG except when EPA was fed at the highest level, when the incorporation was greater when it was fed in the sn-2 than in the sn-1(3) position. There was no significant effect of dietary DHA on monocyte or neutrophil phagocytic activity. Dietary EPA dose-dependently decreased the number of monocytes and neutrophils performing phagocytosis. However, when EPA was fed in the sn-2 position, the ability of active monocytes or neutrophils to engulf bacteria was increased in a dose-dependent fashion. This did not occur when EPA was fed in the sn-1(3) position. Thus, there appears to be an influence of the position of EPA, but not of DHA, in dietary TAG on its incorporation into cell phospholipids and on the activity of phagocytic cells.

AB - The effects of altering the type of n-3 polyunsaturated fatty acid (PUFA) in the mouse diet on the ability of monocytes and neutrophils to perform phagocytosis were investigated. Male weanling mice were fed for 7 d on one of nine diets which contained 178 g lipid/kg and which differed in the type of n-3 PUFA and in the position of these in dietary triacylglycerol (TAG). The control diet contained 4-4 g a-linolenic acid/100g total fatty acids. In the other diets, eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA) replaced a proportion (50 or 100 %) of the a-linolenic acid, and were in the sn-2 or the sn-1(3) position of dietary TAG. There were significant increases in the content of n-3 PUFA in spleen-cell phospholipids when EPA or DHA was fed. These increases were largely independent of the position of EPA or DHA in dietary TAG except when EPA was fed at the highest level, when the incorporation was greater when it was fed in the sn-2 than in the sn-1(3) position. There was no significant effect of dietary DHA on monocyte or neutrophil phagocytic activity. Dietary EPA dose-dependently decreased the number of monocytes and neutrophils performing phagocytosis. However, when EPA was fed in the sn-2 position, the ability of active monocytes or neutrophils to engulf bacteria was increased in a dose-dependent fashion. This did not occur when EPA was fed in the sn-1(3) position. Thus, there appears to be an influence of the position of EPA, but not of DHA, in dietary TAG on its incorporation into cell phospholipids and on the activity of phagocytic cells.

KW - polyunsaturated fatty acids

KW - fish oil

KW - phagocytosis

KW - immune function

KW - monocytes

KW - LONG-CHAIN N-3

KW - KILLER-CELL ACTIVITY

KW - SPRAGUE-DAWLEY RATS

KW - FISH-OIL

KW - DIETARY SUPPLEMENTATION

KW - LYMPHOCYTE FUNCTIONS

KW - MACROPHAGES

KW - LIPIDS

KW - PROLIFERATION

KW - MANIPULATION

U2 - 10.1079/BJN2003996

DO - 10.1079/BJN2003996

M3 - Article

VL - 90

SP - 1071

EP - 1080

JO - British Journal of Nutrition

JF - British Journal of Nutrition

SN - 0007-1145

ER -