The effect of glycyrrhizin on rainbow trout, Oncorhynchus mykiss (Walbaum), leucocyte responses

S. I. Jang, M. J. Marsden, Y. G. Kim, M. S. Choi, C. J. Secombes

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Abstract. Treatment of rainbow trout macrophages with glycyrrhizin (GL), an aqueous extract of licorice, Glycyrrhiza glabra, enhanced their respiratory burst activity. Maximal effects were seen using concentrations of 10–100 μml−1. GL also modulated trout lymphocytes, increasing proliferation responses to the mitogen phytohaemagglutinin some two‐fold over a range of GL concentrations. In addition, GL elicited the release of a macrophage activating factor (MAF) from head kidney leucocytes, as assessed by the ability of generated supernatants to increase respiratory burst activity of target macrophages. MAF activity was most apparent using 100 μg ml−1 GL to induce MAF release and a 48‐h incubation period with the target macrophages. Finally, GL was shown to enhance the release of MAF in response to the mitogen concanavalin A. The possible use of GL as a stimulant of fish innate defences is discussed.

Original languageEnglish
Pages (from-to)307-315
Number of pages9
JournalJournal of Fish Diseases
Volume18
Issue number4
DOIs
Publication statusPublished - 1 Jan 1995

Fingerprint

glycyrrhizin
Glycyrrhizic Acid
Oncorhynchus mykiss
rainbow
Macrophage-Activating Factors
leukocytes
macrophages
Leukocytes
incubation
fish
Glycyrrhiza
Respiratory Burst
Macrophages
Mitogens
Head Kidney
Glycyrrhiza glabra
licorice
Trout
phytohemagglutinin
Phytohemagglutinins

ASJC Scopus subject areas

  • Aquatic Science
  • veterinary (miscalleneous)

Cite this

The effect of glycyrrhizin on rainbow trout, Oncorhynchus mykiss (Walbaum), leucocyte responses. / Jang, S. I.; Marsden, M. J.; Kim, Y. G.; Choi, M. S.; Secombes, C. J.

In: Journal of Fish Diseases, Vol. 18, No. 4, 01.01.1995, p. 307-315.

Research output: Contribution to journalArticle

Jang, S. I. ; Marsden, M. J. ; Kim, Y. G. ; Choi, M. S. ; Secombes, C. J. / The effect of glycyrrhizin on rainbow trout, Oncorhynchus mykiss (Walbaum), leucocyte responses. In: Journal of Fish Diseases. 1995 ; Vol. 18, No. 4. pp. 307-315.
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