Abstract
The effect of increasing dietary intakes of polyunsaturated fatty acids (PUFAs) and vitamin E on indices of oxidative DNA damage was investigated. Twenty-one healthy male, nonsmokers aged 28.9 +/- 1.3 years participated in a free-living, split plot/change over trial in which half the volunteers consumed diets containing 5% PUFA as food energy for 4 wk and, after a 10 wk washout period, consumed a 15% PUFA diet for another 4 wk. The other volunteers followed an identical protocol, except that they consumed the 15% PUFA diet first. The diets were provided to volunteers either with or without an additional 80 mg d alpha-tocopherol acetate/day; otherwise total fat, carbohydrates, protein, and basal vitamin E contents remained unchanged. DNA damage induced by 200 mu M H2O2 in lymphocytes from volunteers as well as endogenous DNA damage in the form of oxidized pyrimidines, measured by alkaline single-cell gel electrophoresis (the comet assay), significantly decreased after consumption of the 5% PUFA diet (P<0.001 and P=0.01, respectively), but significantly increased after consumption of the 15% PUFA diet when cx-tocopherol levels were in the range of 5-7 mg/day (P=0.008 and P=0.03, respectively). These changes were abolished by an additional 80 mg d alpha-tocopherol/day. This study indicates that increasing dietary levels of PUFA to 15% may adversely affect some indices of DNA stability. However, increasing the dietary intake of vitamin E by 80 mg/day ameliorates the damaging effects of PUFA.
Original language | English |
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Pages (from-to) | 2138-2142 |
Number of pages | 5 |
Journal | The FASEB Journal |
Volume | 13 |
Issue number | 15 |
Publication status | Published - 1 Dec 1999 |
Keywords
- PUFA
- lipid peroxidation
- comet assay
- oxidized pyrimidines
- dietary
- adducts
- cancer