The Effect of Insulin Supression on Postprandial Nutrient Metabolism

Studies with infusion of somatostatin and insulin

K C MCHARDY, M A MCNURLAN, Eric Milne, Alexander Graham Calder, L M FEARNS, J BROOM, P J GARLICK

    Research output: Contribution to journalArticle

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    Abstract

    Utilization of fat, carbohydrate and protein before and after feeding was studied in six healthy subjects using simultaneous respiratory gas exchange measurement and [1-C-13] leucine infusion. The role of insulin was investigated by repeating a control study with the addition of an infusion of somatostatin, a hormone which can suppress insulin release. Where near-complete insulin suppression was effected, subjects were studied on a third occasion with the further addition of exogenous insulin infusion. The normal switch on feeding from fat to carbohydrate as principal energy source was reproduced at insulin levels of only 17%-33% of control values, which were inadequate to prevent hyperglycaemia. At fed levels below 10%, a fat-predominant pattern persisted unless insulin was infused. Protein degradation was reduced and synthesis unaffected by feeding, regardless of insulin concentration. Leucine oxidation was dependent on its plasma concentration in the presence of circulating insulin. Thus insulin appears to be necessary for the normal switch to carbohydrate oxidation on feeding but not for postprandial changes in protein metabolism.

    Original languageEnglish
    Pages (from-to)515-526
    Number of pages12
    JournalEuropean Journal of Clinical Nutrition
    Volume45
    Issue number11
    Publication statusPublished - Nov 1991

    Keywords

    • muscle protein-synthesis
    • whole-body leucine
    • amino-acids
    • postabsorptive rats
    • glucose-oxidation
    • diabetic-patients
    • oral glucose
    • secretion
    • carbohydrate
    • hyperinsulinemia

    Cite this

    MCHARDY, K. C., MCNURLAN, M. A., Milne, E., Calder, A. G., FEARNS, L. M., BROOM, J., & GARLICK, P. J. (1991). The Effect of Insulin Supression on Postprandial Nutrient Metabolism: Studies with infusion of somatostatin and insulin. European Journal of Clinical Nutrition, 45(11), 515-526.

    The Effect of Insulin Supression on Postprandial Nutrient Metabolism : Studies with infusion of somatostatin and insulin. / MCHARDY, K C ; MCNURLAN, M A ; Milne, Eric; Calder, Alexander Graham; FEARNS, L M ; BROOM, J ; GARLICK, P J .

    In: European Journal of Clinical Nutrition, Vol. 45, No. 11, 11.1991, p. 515-526.

    Research output: Contribution to journalArticle

    MCHARDY, KC, MCNURLAN, MA, Milne, E, Calder, AG, FEARNS, LM, BROOM, J & GARLICK, PJ 1991, 'The Effect of Insulin Supression on Postprandial Nutrient Metabolism: Studies with infusion of somatostatin and insulin', European Journal of Clinical Nutrition, vol. 45, no. 11, pp. 515-526.
    MCHARDY, K C ; MCNURLAN, M A ; Milne, Eric ; Calder, Alexander Graham ; FEARNS, L M ; BROOM, J ; GARLICK, P J . / The Effect of Insulin Supression on Postprandial Nutrient Metabolism : Studies with infusion of somatostatin and insulin. In: European Journal of Clinical Nutrition. 1991 ; Vol. 45, No. 11. pp. 515-526.
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    abstract = "Utilization of fat, carbohydrate and protein before and after feeding was studied in six healthy subjects using simultaneous respiratory gas exchange measurement and [1-C-13] leucine infusion. The role of insulin was investigated by repeating a control study with the addition of an infusion of somatostatin, a hormone which can suppress insulin release. Where near-complete insulin suppression was effected, subjects were studied on a third occasion with the further addition of exogenous insulin infusion. The normal switch on feeding from fat to carbohydrate as principal energy source was reproduced at insulin levels of only 17{\%}-33{\%} of control values, which were inadequate to prevent hyperglycaemia. At fed levels below 10{\%}, a fat-predominant pattern persisted unless insulin was infused. Protein degradation was reduced and synthesis unaffected by feeding, regardless of insulin concentration. Leucine oxidation was dependent on its plasma concentration in the presence of circulating insulin. Thus insulin appears to be necessary for the normal switch to carbohydrate oxidation on feeding but not for postprandial changes in protein metabolism.",
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    AB - Utilization of fat, carbohydrate and protein before and after feeding was studied in six healthy subjects using simultaneous respiratory gas exchange measurement and [1-C-13] leucine infusion. The role of insulin was investigated by repeating a control study with the addition of an infusion of somatostatin, a hormone which can suppress insulin release. Where near-complete insulin suppression was effected, subjects were studied on a third occasion with the further addition of exogenous insulin infusion. The normal switch on feeding from fat to carbohydrate as principal energy source was reproduced at insulin levels of only 17%-33% of control values, which were inadequate to prevent hyperglycaemia. At fed levels below 10%, a fat-predominant pattern persisted unless insulin was infused. Protein degradation was reduced and synthesis unaffected by feeding, regardless of insulin concentration. Leucine oxidation was dependent on its plasma concentration in the presence of circulating insulin. Thus insulin appears to be necessary for the normal switch to carbohydrate oxidation on feeding but not for postprandial changes in protein metabolism.

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