The effect of intraperitoneally administered recombinant IL-1b on immune parameters and resistance to Aeromonas salmonicida in the rainbow trout (Oncorhynchus mykiss)

Suhee Hong, Scott Peddie, Juan J Campos-Perez, Jun Zou, Christopher J Secombes

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

The present work provides the first information concerning the immunostimulatory activity of trout recombinant interleukin-1 beta (rIL-1beta) in vivo. The predicted rainbow trout mature IL-1beta peptide was produced as a recombinant protein in Escherichia coli. Optimal migration of peritoneal leucocytes and rIL-1beta induced phagocytosis occurred following intraperitoneal injection of 1 mug of the recombinant protein. Moreover, systemic IL-1beta, COX-2 and lysozyme 11 gene expression was enhanced following greater than or equal to 1 mug rIL-1beta administration. Finally, resistance to Aeromonas salmonicida, the causative agent of furunculosis, was augmented at early times (2 days) post-injection of 1 mug rIL-1beta. (C) 2003 Elsevier Science Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)801-812
Number of pages12
JournalDevelopmental and Comparative Immunology
Volume27
Issue number9
Early online date25 Mar 2003
DOIs
Publication statusPublished - Oct 2003

Keywords

  • interleukin-1 beta
  • rainbow trout
  • recombinant protein
  • peritoneal exudate cells
  • phagocytosis
  • gene expression
  • disease resistance
  • immunomodulation
  • leukocytes in-vitro
  • Atlantic salmon
  • biological-activity
  • ovine IL-1-beta
  • cytokine
  • interleukin-1-beta
  • macrophages
  • lysozyme
  • cells

Cite this

The effect of intraperitoneally administered recombinant IL-1b on immune parameters and resistance to Aeromonas salmonicida in the rainbow trout (Oncorhynchus mykiss). / Hong, Suhee; Peddie, Scott; Campos-Perez, Juan J; Zou, Jun; Secombes, Christopher J.

In: Developmental and Comparative Immunology, Vol. 27, No. 9, 10.2003, p. 801-812.

Research output: Contribution to journalArticle

@article{99f99beef2fa4e88bb6e72162bad7262,
title = "The effect of intraperitoneally administered recombinant IL-1b on immune parameters and resistance to Aeromonas salmonicida in the rainbow trout (Oncorhynchus mykiss)",
abstract = "The present work provides the first information concerning the immunostimulatory activity of trout recombinant interleukin-1 beta (rIL-1beta) in vivo. The predicted rainbow trout mature IL-1beta peptide was produced as a recombinant protein in Escherichia coli. Optimal migration of peritoneal leucocytes and rIL-1beta induced phagocytosis occurred following intraperitoneal injection of 1 mug of the recombinant protein. Moreover, systemic IL-1beta, COX-2 and lysozyme 11 gene expression was enhanced following greater than or equal to 1 mug rIL-1beta administration. Finally, resistance to Aeromonas salmonicida, the causative agent of furunculosis, was augmented at early times (2 days) post-injection of 1 mug rIL-1beta. (C) 2003 Elsevier Science Ltd. All rights reserved.",
keywords = "interleukin-1 beta, rainbow trout, recombinant protein, peritoneal exudate cells, phagocytosis, gene expression, disease resistance, immunomodulation, leukocytes in-vitro, Atlantic salmon, biological-activity, ovine IL-1-beta, cytokine, interleukin-1-beta, macrophages, lysozyme, cells",
author = "Suhee Hong and Scott Peddie and Campos-Perez, {Juan J} and Jun Zou and Secombes, {Christopher J}",
year = "2003",
month = "10",
doi = "10.1016/S0145-305X(03)00056-9",
language = "English",
volume = "27",
pages = "801--812",
journal = "Developmental and Comparative Immunology",
issn = "0145-305X",
publisher = "Elsevier Limited",
number = "9",

}

TY - JOUR

T1 - The effect of intraperitoneally administered recombinant IL-1b on immune parameters and resistance to Aeromonas salmonicida in the rainbow trout (Oncorhynchus mykiss)

AU - Hong, Suhee

AU - Peddie, Scott

AU - Campos-Perez, Juan J

AU - Zou, Jun

AU - Secombes, Christopher J

PY - 2003/10

Y1 - 2003/10

N2 - The present work provides the first information concerning the immunostimulatory activity of trout recombinant interleukin-1 beta (rIL-1beta) in vivo. The predicted rainbow trout mature IL-1beta peptide was produced as a recombinant protein in Escherichia coli. Optimal migration of peritoneal leucocytes and rIL-1beta induced phagocytosis occurred following intraperitoneal injection of 1 mug of the recombinant protein. Moreover, systemic IL-1beta, COX-2 and lysozyme 11 gene expression was enhanced following greater than or equal to 1 mug rIL-1beta administration. Finally, resistance to Aeromonas salmonicida, the causative agent of furunculosis, was augmented at early times (2 days) post-injection of 1 mug rIL-1beta. (C) 2003 Elsevier Science Ltd. All rights reserved.

AB - The present work provides the first information concerning the immunostimulatory activity of trout recombinant interleukin-1 beta (rIL-1beta) in vivo. The predicted rainbow trout mature IL-1beta peptide was produced as a recombinant protein in Escherichia coli. Optimal migration of peritoneal leucocytes and rIL-1beta induced phagocytosis occurred following intraperitoneal injection of 1 mug of the recombinant protein. Moreover, systemic IL-1beta, COX-2 and lysozyme 11 gene expression was enhanced following greater than or equal to 1 mug rIL-1beta administration. Finally, resistance to Aeromonas salmonicida, the causative agent of furunculosis, was augmented at early times (2 days) post-injection of 1 mug rIL-1beta. (C) 2003 Elsevier Science Ltd. All rights reserved.

KW - interleukin-1 beta

KW - rainbow trout

KW - recombinant protein

KW - peritoneal exudate cells

KW - phagocytosis

KW - gene expression

KW - disease resistance

KW - immunomodulation

KW - leukocytes in-vitro

KW - Atlantic salmon

KW - biological-activity

KW - ovine IL-1-beta

KW - cytokine

KW - interleukin-1-beta

KW - macrophages

KW - lysozyme

KW - cells

U2 - 10.1016/S0145-305X(03)00056-9

DO - 10.1016/S0145-305X(03)00056-9

M3 - Article

VL - 27

SP - 801

EP - 812

JO - Developmental and Comparative Immunology

JF - Developmental and Comparative Immunology

SN - 0145-305X

IS - 9

ER -