The effect of intraperitoneally administered recombinant IL-1b on immune parameters and resistance to Aeromonas salmonicida in the rainbow trout (Oncorhynchus mykiss)

Suhee Hong, Scott Peddie, Juan J Campos-Perez, Jun Zou, Christopher J Secombes

Research output: Contribution to journalArticlepeer-review

68 Citations (Scopus)


The present work provides the first information concerning the immunostimulatory activity of trout recombinant interleukin-1 beta (rIL-1beta) in vivo. The predicted rainbow trout mature IL-1beta peptide was produced as a recombinant protein in Escherichia coli. Optimal migration of peritoneal leucocytes and rIL-1beta induced phagocytosis occurred following intraperitoneal injection of 1 mug of the recombinant protein. Moreover, systemic IL-1beta, COX-2 and lysozyme 11 gene expression was enhanced following greater than or equal to 1 mug rIL-1beta administration. Finally, resistance to Aeromonas salmonicida, the causative agent of furunculosis, was augmented at early times (2 days) post-injection of 1 mug rIL-1beta. (C) 2003 Elsevier Science Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)801-812
Number of pages12
JournalDevelopmental and Comparative Immunology
Issue number9
Early online date25 Mar 2003
Publication statusPublished - Oct 2003


  • interleukin-1 beta
  • rainbow trout
  • recombinant protein
  • peritoneal exudate cells
  • phagocytosis
  • gene expression
  • disease resistance
  • immunomodulation
  • leukocytes in-vitro
  • Atlantic salmon
  • biological-activity
  • ovine IL-1-beta
  • cytokine
  • interleukin-1-beta
  • macrophages
  • lysozyme
  • cells

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