The effect of intraperitoneally administered recombinant IL-1b on immune parameters and resistance to Aeromonas salmonicida in the rainbow trout (Oncorhynchus mykiss)

Suhee Hong; Scott Peddie; Juan J Campos-Perez; Jun Zou; Christopher J Secombes

doi:10.1016/S0145-305X(03)00056-9

The effect of intraperitoneally administered recombinant IL-1b on immune parameters and resistance to Aeromonas salmonicida in the rainbow trout (Oncorhynchus mykiss)

Suhee Hong, Scott Peddie, Juan J Campos-Perez, Jun Zou, Christopher J Secombes

Research output: Contribution to journal › Article › peer-review

82 Citations (Scopus)

Abstract

The present work provides the first information concerning the immunostimulatory activity of trout recombinant interleukin-1 beta (rIL-1beta) in vivo. The predicted rainbow trout mature IL-1beta peptide was produced as a recombinant protein in Escherichia coli. Optimal migration of peritoneal leucocytes and rIL-1beta induced phagocytosis occurred following intraperitoneal injection of 1 mug of the recombinant protein. Moreover, systemic IL-1beta, COX-2 and lysozyme 11 gene expression was enhanced following greater than or equal to 1 mug rIL-1beta administration. Finally, resistance to Aeromonas salmonicida, the causative agent of furunculosis, was augmented at early times (2 days) post-injection of 1 mug rIL-1beta. (C) 2003 Elsevier Science Ltd. All rights reserved.

Original language	English
Pages (from-to)	801-812
Number of pages	12
Journal	Developmental and Comparative Immunology
Volume	27
Issue number	9
Early online date	25 Mar 2003
DOIs	https://doi.org/10.1016/S0145-305X(03)00056-9
Publication status	Published - Oct 2003

Keywords

interleukin-1 beta
rainbow trout
recombinant protein
peritoneal exudate cells
phagocytosis
gene expression
disease resistance
immunomodulation
leukocytes in-vitro
Atlantic salmon
biological-activity
ovine IL-1-beta
cytokine
interleukin-1-beta
macrophages
lysozyme
cells

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10.1016/S0145-305X(03)00056-9

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The effect of intraperitoneally administered recombinant IL-1b on immune parameters and resistance to Aeromonas salmonicida in the rainbow trout (Oncorhynchus mykiss). / Hong, Suhee; Peddie, Scott; Campos-Perez, Juan J et al.

In: Developmental and Comparative Immunology, Vol. 27, No. 9, 10.2003, p. 801-812.

Research output: Contribution to journal › Article › peer-review

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title = "The effect of intraperitoneally administered recombinant IL-1b on immune parameters and resistance to Aeromonas salmonicida in the rainbow trout (Oncorhynchus mykiss)",

abstract = "The present work provides the first information concerning the immunostimulatory activity of trout recombinant interleukin-1 beta (rIL-1beta) in vivo. The predicted rainbow trout mature IL-1beta peptide was produced as a recombinant protein in Escherichia coli. Optimal migration of peritoneal leucocytes and rIL-1beta induced phagocytosis occurred following intraperitoneal injection of 1 mug of the recombinant protein. Moreover, systemic IL-1beta, COX-2 and lysozyme 11 gene expression was enhanced following greater than or equal to 1 mug rIL-1beta administration. Finally, resistance to Aeromonas salmonicida, the causative agent of furunculosis, was augmented at early times (2 days) post-injection of 1 mug rIL-1beta. (C) 2003 Elsevier Science Ltd. All rights reserved.",

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AU - Zou, Jun

AU - Secombes, Christopher J

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AB - The present work provides the first information concerning the immunostimulatory activity of trout recombinant interleukin-1 beta (rIL-1beta) in vivo. The predicted rainbow trout mature IL-1beta peptide was produced as a recombinant protein in Escherichia coli. Optimal migration of peritoneal leucocytes and rIL-1beta induced phagocytosis occurred following intraperitoneal injection of 1 mug of the recombinant protein. Moreover, systemic IL-1beta, COX-2 and lysozyme 11 gene expression was enhanced following greater than or equal to 1 mug rIL-1beta administration. Finally, resistance to Aeromonas salmonicida, the causative agent of furunculosis, was augmented at early times (2 days) post-injection of 1 mug rIL-1beta. (C) 2003 Elsevier Science Ltd. All rights reserved.

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KW - recombinant protein

KW - peritoneal exudate cells

KW - phagocytosis

KW - gene expression

KW - disease resistance

KW - immunomodulation

KW - leukocytes in-vitro

KW - Atlantic salmon

KW - biological-activity

KW - ovine IL-1-beta

KW - cytokine

KW - interleukin-1-beta

KW - macrophages

KW - lysozyme

KW - cells

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The effect of intraperitoneally administered recombinant IL-1b on immune parameters and resistance to Aeromonas salmonicida in the rainbow trout (Oncorhynchus mykiss)

Abstract

Keywords

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