The effect of Ku on telomere replication time is mediated by telomere length but is independent of histone tail acetylation

Huiyong Lian, Euan Douglas Robertson, Shinichiro Hiraga, Gina M. Alvino, David Collingwood, H. J. McCune, Akila Sridhar, Bonita J. Brewer, M. K. Raghuraman, Anne Dunlop Donaldson

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Abstract

DNA replication in Saccharomyces cerevisiae proceeds according to a temporal program. We have investigated the role of the telomere-binding Ku complex in specifying late replication of telomere-proximal sequences. Genome-wide analysis shows that regions extending up to 80 kb from telomeres replicate abnormally early in a yku70 mutant. We find that Ku does not appear to regulate replication time by binding replication origins directly, nor is its effect on telomere replication timing mediated by histone tail acetylation. We show that Ku instead regulates replication timing through its effect on telomere length, because deletion of the telomerase regulator Pif1 largely reverses the short telomere defect of a yku70 mutant and simultaneously rescues its replication timing defect. Consistent with this conclusion, deleting the genome integrity component Elg1 partially rescued both length and replication timing of yku70 telomeres. Telomere length-mediated control of replication timing requires the TG(1-3) repeat-counting component Rif1, because a rif1 mutant replicates telomeric regions early, despite having extended TG(1-3) tracts. Overall, our results suggest that the effect of Ku on telomere replication timing results from its impact on TG(1-3) repeat length and support a model in which Rif1 measures telomere repeat length to ensure that telomere replication timing is correctly programmed.
Original languageEnglish
Pages (from-to)1753-1756
Number of pages4
JournalMolecular Biology of the Cell
Volume22
Issue number10
Early online date25 Mar 2011
DOIs
Publication statusPublished - 15 May 2011

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Telomere
Acetylation
Histones
Genome Components
Replication Origin
Telomerase
DNA Replication
Saccharomyces cerevisiae
Genome

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The effect of Ku on telomere replication time is mediated by telomere length but is independent of histone tail acetylation. / Lian, Huiyong; Robertson, Euan Douglas; Hiraga, Shinichiro; Alvino, Gina M.; Collingwood, David; McCune, H. J.; Sridhar, Akila; Brewer, Bonita J.; Raghuraman, M. K.; Donaldson, Anne Dunlop.

In: Molecular Biology of the Cell, Vol. 22, No. 10, 15.05.2011, p. 1753-1756.

Research output: Contribution to journalArticle

Lian, H, Robertson, ED, Hiraga, S, Alvino, GM, Collingwood, D, McCune, HJ, Sridhar, A, Brewer, BJ, Raghuraman, MK & Donaldson, AD 2011, 'The effect of Ku on telomere replication time is mediated by telomere length but is independent of histone tail acetylation', Molecular Biology of the Cell, vol. 22, no. 10, pp. 1753-1756. https://doi.org/10.1091/mbc.E10-06-0549
Lian, Huiyong ; Robertson, Euan Douglas ; Hiraga, Shinichiro ; Alvino, Gina M. ; Collingwood, David ; McCune, H. J. ; Sridhar, Akila ; Brewer, Bonita J. ; Raghuraman, M. K. ; Donaldson, Anne Dunlop. / The effect of Ku on telomere replication time is mediated by telomere length but is independent of histone tail acetylation. In: Molecular Biology of the Cell. 2011 ; Vol. 22, No. 10. pp. 1753-1756.
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