The Effect of Oligohydramnios on Tissue and Cellular Morphometry in the Developing Fetal Murine Lung

Tanbir Najrana; Juan Sanchez-Esteban; Rasha Abu Eid

doi:10.1109/CSCS.2017.52

The Effect of Oligohydramnios on Tissue and Cellular Morphometry in the Developing Fetal Murine Lung

Tanbir Najrana, Juan Sanchez-Esteban, Rasha Abu Eid

Dental Education

Brown University

Research output: Chapter in Book/Report/Conference proceeding › Published conference contribution

1 Citation (Scopus)

Abstract

Mechanical forces play important roles in fetal lung development, therefore, pulmonary hypoplasia is an expected response to Oligohydramnios (OH) due to the decrease of fluid pressure. Amniotic sacs were punctured in pregnant mice with untouched fetuses serving as controls. Fetuses were delivered, and lung tissues collected. Histological sections were imaged and analyzed at the tissue level and the cellular level. Various morphometric descriptors of size, shape and differentiation were used to compare tissues from OH and untouched controls. Our results show that OH leads to smaller, less developed air spaces when compared to controls. Furthermore, cells from OH fetuses are smaller and less regular in shape compared to controls. There is also a defect in the differentiation of Type I alveolar epithelial cells. Here, we show a significant change in the morphometry of the fetal lung as a result of OH. These changes are consistent with the observed pulmonary hypoplasia and are important objective parameters that help in elucidating mechanisms of lung development and potential management of OH induced pulmonary hypoplasia.

Original language	English
Title of host publication	2017 21st International Conference on Control Systems and Computer Science (CSCS)
Editors	Ioan Dumitrache, Adina Magda Florea, Florin Pop, Alexandru Dumitrașcu
Place of Publication	Bucharest, Romania
Publisher	IEEE Explore
Pages	1-6
Number of pages	6
ISBN (Print)	9781538618400
DOIs	https://doi.org/10.1109/CSCS.2017.52
Publication status	Published - 7 Jul 2017
Event	21st International Conference on Control Systems and Computer Science (CSCS21) - Bucharest, Romania Duration: 29 May 2017 → 31 May 2017

Conference

Conference	21st International Conference on Control Systems and Computer Science (CSCS21)
Country/Territory	Romania
City	Bucharest
Period	29/05/17 → 31/05/17

Bibliographical note

This work was supported by funding from the National Institute of General Medical Sciences of the National Institutes of Health, Number: P30GM114750; Department of Pediatrics; Kilguss Research Core of Women & Infants Hospital of Rhode Island.

Keywords

Oligohydramnios
image analysis
morphometry
fractal dimension
pulmonary hypoplasia

Access to Document

10.1109/CSCS.2017.52Licence: Unspecified

Cite this

The Effect of Oligohydramnios on Tissue and Cellular Morphometry in the Developing Fetal Murine Lung. / Najrana, Tanbir; Sanchez-Esteban, Juan; Abu Eid, Rasha.
2017 21st International Conference on Control Systems and Computer Science (CSCS). ed. / Ioan Dumitrache; Adina Magda Florea; Florin Pop; Alexandru Dumitrașcu. Bucharest, Romania: IEEE Explore, 2017. p. 1-6.

Research output: Chapter in Book/Report/Conference proceeding › Published conference contribution

Najrana, T, Sanchez-Esteban, J & Abu Eid, R 2017, The Effect of Oligohydramnios on Tissue and Cellular Morphometry in the Developing Fetal Murine Lung. in I Dumitrache, AM Florea, F Pop & A Dumitrașcu (eds), 2017 21st International Conference on Control Systems and Computer Science (CSCS). IEEE Explore, Bucharest, Romania, pp. 1-6, 21st International Conference on Control Systems and Computer Science (CSCS21), Bucharest, Romania, 29/05/17. https://doi.org/10.1109/CSCS.2017.52

Najrana, Tanbir ; Sanchez-Esteban, Juan ; Abu Eid, Rasha. / The Effect of Oligohydramnios on Tissue and Cellular Morphometry in the Developing Fetal Murine Lung. 2017 21st International Conference on Control Systems and Computer Science (CSCS). editor / Ioan Dumitrache ; Adina Magda Florea ; Florin Pop ; Alexandru Dumitrașcu. Bucharest, Romania : IEEE Explore, 2017. pp. 1-6

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abstract = "Mechanical forces play important roles in fetal lung development, therefore, pulmonary hypoplasia is an expected response to Oligohydramnios (OH) due to the decrease of fluid pressure. Amniotic sacs were punctured in pregnant mice with untouched fetuses serving as controls. Fetuses were delivered, and lung tissues collected. Histological sections were imaged and analyzed at the tissue level and the cellular level. Various morphometric descriptors of size, shape and differentiation were used to compare tissues from OH and untouched controls. Our results show that OH leads to smaller, less developed air spaces when compared to controls. Furthermore, cells from OH fetuses are smaller and less regular in shape compared to controls. There is also a defect in the differentiation of Type I alveolar epithelial cells. Here, we show a significant change in the morphometry of the fetal lung as a result of OH. These changes are consistent with the observed pulmonary hypoplasia and are important objective parameters that help in elucidating mechanisms of lung development and potential management of OH induced pulmonary hypoplasia.",

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N2 - Mechanical forces play important roles in fetal lung development, therefore, pulmonary hypoplasia is an expected response to Oligohydramnios (OH) due to the decrease of fluid pressure. Amniotic sacs were punctured in pregnant mice with untouched fetuses serving as controls. Fetuses were delivered, and lung tissues collected. Histological sections were imaged and analyzed at the tissue level and the cellular level. Various morphometric descriptors of size, shape and differentiation were used to compare tissues from OH and untouched controls. Our results show that OH leads to smaller, less developed air spaces when compared to controls. Furthermore, cells from OH fetuses are smaller and less regular in shape compared to controls. There is also a defect in the differentiation of Type I alveolar epithelial cells. Here, we show a significant change in the morphometry of the fetal lung as a result of OH. These changes are consistent with the observed pulmonary hypoplasia and are important objective parameters that help in elucidating mechanisms of lung development and potential management of OH induced pulmonary hypoplasia.

AB - Mechanical forces play important roles in fetal lung development, therefore, pulmonary hypoplasia is an expected response to Oligohydramnios (OH) due to the decrease of fluid pressure. Amniotic sacs were punctured in pregnant mice with untouched fetuses serving as controls. Fetuses were delivered, and lung tissues collected. Histological sections were imaged and analyzed at the tissue level and the cellular level. Various morphometric descriptors of size, shape and differentiation were used to compare tissues from OH and untouched controls. Our results show that OH leads to smaller, less developed air spaces when compared to controls. Furthermore, cells from OH fetuses are smaller and less regular in shape compared to controls. There is also a defect in the differentiation of Type I alveolar epithelial cells. Here, we show a significant change in the morphometry of the fetal lung as a result of OH. These changes are consistent with the observed pulmonary hypoplasia and are important objective parameters that help in elucidating mechanisms of lung development and potential management of OH induced pulmonary hypoplasia.

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A2 - Florea, Adina Magda

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ER -

The Effect of Oligohydramnios on Tissue and Cellular Morphometry in the Developing Fetal Murine Lung

Abstract

Conference

Bibliographical note

Keywords

Access to Document

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Cite this