The effect of Pediococcus acidilactici on the gut microbiota and immune status of on-growing red tilapia (Oreochromis niloticus)

R M W Ferguson, D L Merrifield, G M Harper, M D Rawling, S Mustafa, S Picchietti, J L Balcázar, S J Davies

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137 Citations (Scopus)

Abstract

Aim:  To assess Pediococcus acidilactici as a dietary supplement for on-growing red tilapia (Oreochromis niloticus).

Methods and Results:  Tilapia were fed either a control diet or control diet supplemented with Ped. acidilactici at 107 CFU g−1 for 32 days. Ped. acidilactici colonized the intestinal tract and significantly affected the intestinal microbial communities. PCR-DGGE revealed direct antagonism of gastric Ped. acidilactici with an endogenous uncultured bacterium during a period of reverting to nonsupplemented feeding. Light microscopy revealed that gut integrity and leucocyte levels were unaffected by Ped. acidilactici; however, blood leucocyte levels and serum lysozyme activity were elevated after 14-days' feeding. No significant improvements in growth performance were observed at the end of the trial (day 32), but survival was significantly higher in the probiotic group.

Conclusions:  The study demonstrates that oral supplementation of Ped. acidilactici modulates intestinal bacterial communities in on-growing red tilapia and also stimulates some aspects of the nonspecific immune response.

Significance and Impact of the study:  To our knowledge this is the first study assessing the effects of probiotics on the gut microbiota of tilapia using culture-independent methods. Such methods are crucial to understand the mechanisms which underpin and mediate host benefits.
Original languageEnglish
Pages (from-to)851-862
Number of pages12
JournalJournal of Applied Microbiology
Volume109
Issue number3
Early online date6 Mar 2010
DOIs
Publication statusPublished - Sep 2010

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    Ferguson, R. M. W., Merrifield, D. L., Harper, G. M., Rawling, M. D., Mustafa, S., Picchietti, S., Balcázar, J. L., & Davies, S. J. (2010). The effect of Pediococcus acidilactici on the gut microbiota and immune status of on-growing red tilapia (Oreochromis niloticus). Journal of Applied Microbiology, 109(3), 851-862. https://doi.org/10.1111/j.1365-2672.2010.04713.x