The whole cell patch clamp technique was used to study the effects of changes in [Ca2+](i) on the excitability of cultured dorsal root ganglion (DRG) neurones. Increases in [Ca2+](i) caused membrane depolarization, altered the characteristics of evoked action potentials and activated potassium, chloride and non-selective cation conductances. Mobilization of Ca2+ from intracellular stores with caffeine (1 mM) or ryanodine (10 mu M) or photorelease of Ca2+ from DM-nitrophen gave rise to depolarizations suggesting a dominant inward current under our recording conditions of low [Ca2+](i) buffering capacity. The actions of [Ca2+](i) could be partially reversed by intracellular flash photolysis of diazo-2 but not by diazo-3. The electrophysiological properties of some DRG neurones were not influenced by changes in [Ca2+](i) and we suggest that this is because in this heterogeneous culture some neurones do not express Ca2+-activated ion channels. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.
|Number of pages||4|
|Publication status||Published - 1999|
- intracellular flash photolysis
- calcium-activated conductances
- SENSORY NEURONS