The effects of changing intracellular Ca2+ buffering on the excitability of cultured dorsal root ganglion neurones

A Ayar, C Storer, E L Tatham, R H Scott

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The whole cell patch clamp technique was used to study the effects of changes in [Ca2+](i) on the excitability of cultured dorsal root ganglion (DRG) neurones. Increases in [Ca2+](i) caused membrane depolarization, altered the characteristics of evoked action potentials and activated potassium, chloride and non-selective cation conductances. Mobilization of Ca2+ from intracellular stores with caffeine (1 mM) or ryanodine (10 mu M) or photorelease of Ca2+ from DM-nitrophen gave rise to depolarizations suggesting a dominant inward current under our recording conditions of low [Ca2+](i) buffering capacity. The actions of [Ca2+](i) could be partially reversed by intracellular flash photolysis of diazo-2 but not by diazo-3. The electrophysiological properties of some DRG neurones were not influenced by changes in [Ca2+](i) and we suggest that this is because in this heterogeneous culture some neurones do not express Ca2+-activated ion channels. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)171-174
Number of pages4
JournalNeuroscience Letters
Volume271
Publication statusPublished - 1999

Keywords

  • intracellular flash photolysis
  • ryanodine
  • caffeine
  • diazo-2
  • DM-nitrophen
  • calcium-activated conductances
  • SENSORY NEURONS
  • CHELATORS

Cite this

The effects of changing intracellular Ca2+ buffering on the excitability of cultured dorsal root ganglion neurones. / Ayar, A ; Storer, C ; Tatham, E L ; Scott, R H .

In: Neuroscience Letters, Vol. 271, 1999, p. 171-174.

Research output: Contribution to journalArticle

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AB - The whole cell patch clamp technique was used to study the effects of changes in [Ca2+](i) on the excitability of cultured dorsal root ganglion (DRG) neurones. Increases in [Ca2+](i) caused membrane depolarization, altered the characteristics of evoked action potentials and activated potassium, chloride and non-selective cation conductances. Mobilization of Ca2+ from intracellular stores with caffeine (1 mM) or ryanodine (10 mu M) or photorelease of Ca2+ from DM-nitrophen gave rise to depolarizations suggesting a dominant inward current under our recording conditions of low [Ca2+](i) buffering capacity. The actions of [Ca2+](i) could be partially reversed by intracellular flash photolysis of diazo-2 but not by diazo-3. The electrophysiological properties of some DRG neurones were not influenced by changes in [Ca2+](i) and we suggest that this is because in this heterogeneous culture some neurones do not express Ca2+-activated ion channels. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

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