The Effects of Graded Levels of Calorie Restriction

X. Transcriptomic Responses of Epididymal Adipose Tissue

Davina Derous, Sharon E Mitchell, Cara L Green, Yingchun Wang, Jing Dong J Han, Luonan Chen, Daniel E L Promislow, David Lusseau, Alex Douglas, John R Speakman

Research output: Contribution to journalArticle

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Abstract

Calorie restriction (CR) leads to a remarkable decrease in adipose tissue mass and increases longevity in many taxa. Since the discovery of leptin, the secretory abilities of adipose tissue have gained prominence in the responses to CR. We quantified transcripts of epididymal white adipose tissue of male C57BL/6 mice exposed to graded levels of CR (0% to 40% CR) for three months. The numbers of differentially expressed genes (DEGs) involved in NF-κB, HIF1-α and p53 signalling increased with increasing levels of CR. These pathways were all significantly downregulated at 40% CR relative to 12h ad libitum feeding. In addition, graded CR had a substantial impact on DEGs associated with pathways involved in angiogenesis. Of the 497 genes differentially expressed with graded CR, 155 of these genes included a signal peptide motif. These putative signalling proteins were involved in the response to ketones, TGF-β signalling, negative regulation of insulin secretion and inflammation. This accords with the previously established effects of graded CR on glucose homeostasis in the same mice. Overall these data suggest reduced levels of adipose tissue under CR may contribute to the protective impact of CR in multiple ways linked to changes in a large population of secreted proteins.

Original languageEnglish
Pages (from-to)279-288
Number of pages10
JournalThe Journals of Gerontology. Series A, Biological Sciences and Medical Sciences
Volume73
Issue number3
Early online date27 May 2017
DOIs
Publication statusPublished - 2 Mar 2018

Fingerprint

Adipose Tissue
Genes
White Adipose Tissue
Protein Sorting Signals
Leptin
Ketones
Inbred C57BL Mouse
Proteins
Homeostasis
Down-Regulation
Insulin
Inflammation
Glucose
Population

Keywords

  • biology of ageing
  • genetics
  • signalling

Cite this

The Effects of Graded Levels of Calorie Restriction : X. Transcriptomic Responses of Epididymal Adipose Tissue. / Derous, Davina; Mitchell, Sharon E; Green, Cara L; Wang, Yingchun; Han, Jing Dong J; Chen, Luonan; Promislow, Daniel E L; Lusseau, David; Douglas, Alex; Speakman, John R.

In: The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences, Vol. 73, No. 3, 02.03.2018, p. 279-288.

Research output: Contribution to journalArticle

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