The Effects of Graded Levels of Calorie Restriction: XIII. Global Metabolomics Screen Reveals Graded Changes in Circulating Amino Acids, Vitamins, and Bile Acids in the Plasma of C57BL/6 Mice

Cara L Green, Quinlyn A Soltow, Sharon E Mitchell, Davina Derous, Yingchun Wang, Luonan Chen, Jing-Dong J Han, Daniel E. L. Promislow, David Lusseau, Alexander Douglas, Dean P Jones, John R. Speakman

Research output: Contribution to journalArticle

3 Citations (Scopus)
8 Downloads (Pure)

Abstract

Calorie restriction (CR) remains the most robust intervention to extend life span and improve health span. Using a global mass spectrometry–based metabolomics approach, we identified metabolites that were significantly differentially expressed in the plasma of C57BL/6 mice, fed graded levels of calorie restriction (10% CR, 20% CR, 30% CR, and 40% CR) compared with mice fed ad libitum for 12 hours a day. The differential expression of metabolites increased with the severity of CR. Pathway analysis revealed that graded CR had an impact on vitamin E and vitamin B levels, branched chain amino acids, aromatic amino acids, and fatty acid pathways. The majority of amino acids correlated positively with fat-free mass and visceral fat mass, indicating a strong relationship with body composition and vitamin E metabolites correlated with stomach and colon size, which may allude to the beneficial effects of investing in gastrointestinal organs with CR. In addition, metabolites that showed a graded effect, such as the sphinganines, carnitines, and bile acids, match our previous study on liver, which suggests not only that CR remodels the metabolome in a way that promotes energy efficiency, but also that some changes are conserved across tissues.
Original languageEnglish
Pages (from-to)16-26
Number of pages11
JournalThe Journals of Gerontology. Series A, Biological Sciences and Medical Sciences
Volume74
Issue number1
Early online date30 Apr 2018
DOIs
Publication statusPublished - 1 Jan 2019

Keywords

  • metabolomics
  • calorie restriction
  • aging
  • vitamin E
  • amino acids
  • bile acids
  • Metabolomics
  • Bile acids
  • Vitamin E
  • Calorie restriction
  • Aging
  • Amino acids

ASJC Scopus subject areas

  • Geriatrics and Gerontology
  • Ageing

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