The expanding repertoire of the IL-12 cytokine family in teleost fish: Identification of three paralogues each of the p35 and p40 genes in salmonids, and comparative analysis of their expression and modulation in Atlantic salmon Salmo salar

Tiehui Wang, Mansourah Husain

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Interleukin (IL)-12 family cytokines are heterodimers of an alpha-chain (p19, p28 and p35) and a beta-chain (p40 and Ebi3), present as IL-12 (p35/p40), IL-23 (p19/p40), IL-27 (p28/Ebi3) and IL-35 (p35/Ebi3), and play key roles in immune responses in mammals. One p35 and up to three p40 genes have been cloned in some fish species. The identification of three active p35 genes, along with three p40 paralogues in salmonids in the current study further expands the repertoire of IL-12, IL-23 and IL-35 molecules in these species. The multiple p35 genes in teleost fish appear to have arisen via whole genome duplications. The different paralogues of the subunits are divergent, and differentially expressed and modulated by PAMPs and proinflammatory cytokines, hinting that distinct isoforms could be produced in response to infection. Therefore, the expanded IL-12 cytokine family may provide an unprecedented level of regulation to fine tune the immune response in fish. (C) 2014 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)194-207
Number of pages14
JournalDevelopmental and Comparative Immunology
Volume46
Issue number2
Early online date20 Apr 2014
DOIs
Publication statusPublished - Oct 2014

Keywords

  • IL-12
  • IL-23
  • IL-35
  • p35
  • p40
  • Salmonid
  • trout oncorhynchus-mykiss
  • regulatory mechanisms
  • genome duplication
  • adaptive immunity
  • interleukin-12
  • bioactivity
  • cloning
  • protein
  • innate
  • infection

Cite this

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title = "The expanding repertoire of the IL-12 cytokine family in teleost fish: Identification of three paralogues each of the p35 and p40 genes in salmonids, and comparative analysis of their expression and modulation in Atlantic salmon Salmo salar",
abstract = "Interleukin (IL)-12 family cytokines are heterodimers of an alpha-chain (p19, p28 and p35) and a beta-chain (p40 and Ebi3), present as IL-12 (p35/p40), IL-23 (p19/p40), IL-27 (p28/Ebi3) and IL-35 (p35/Ebi3), and play key roles in immune responses in mammals. One p35 and up to three p40 genes have been cloned in some fish species. The identification of three active p35 genes, along with three p40 paralogues in salmonids in the current study further expands the repertoire of IL-12, IL-23 and IL-35 molecules in these species. The multiple p35 genes in teleost fish appear to have arisen via whole genome duplications. The different paralogues of the subunits are divergent, and differentially expressed and modulated by PAMPs and proinflammatory cytokines, hinting that distinct isoforms could be produced in response to infection. Therefore, the expanded IL-12 cytokine family may provide an unprecedented level of regulation to fine tune the immune response in fish. (C) 2014 Elsevier Ltd. All rights reserved.",
keywords = "IL-12, IL-23, IL-35, p35, p40, Salmonid, trout oncorhynchus-mykiss, regulatory mechanisms, genome duplication, adaptive immunity, interleukin-12, bioactivity, cloning, protein, innate, infection",
author = "Tiehui Wang and Mansourah Husain",
note = "TW received funding from the MASTS pooling initiative (The Marine Alliance for Science and Technology for Scotland, Scottish Funding Council Grant no. HR09011). M.H. was financially supported by the Public Authority of Applied Education and Training, Kuwait. We thank Prof. C.J. Secombes (University of Aberdeen) for his support and critical reading of this manuscript.",
year = "2014",
month = "10",
doi = "10.1016/j.dci.2014.04.008",
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journal = "Developmental and Comparative Immunology",
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T1 - The expanding repertoire of the IL-12 cytokine family in teleost fish

T2 - Identification of three paralogues each of the p35 and p40 genes in salmonids, and comparative analysis of their expression and modulation in Atlantic salmon Salmo salar

AU - Wang, Tiehui

AU - Husain, Mansourah

N1 - TW received funding from the MASTS pooling initiative (The Marine Alliance for Science and Technology for Scotland, Scottish Funding Council Grant no. HR09011). M.H. was financially supported by the Public Authority of Applied Education and Training, Kuwait. We thank Prof. C.J. Secombes (University of Aberdeen) for his support and critical reading of this manuscript.

PY - 2014/10

Y1 - 2014/10

N2 - Interleukin (IL)-12 family cytokines are heterodimers of an alpha-chain (p19, p28 and p35) and a beta-chain (p40 and Ebi3), present as IL-12 (p35/p40), IL-23 (p19/p40), IL-27 (p28/Ebi3) and IL-35 (p35/Ebi3), and play key roles in immune responses in mammals. One p35 and up to three p40 genes have been cloned in some fish species. The identification of three active p35 genes, along with three p40 paralogues in salmonids in the current study further expands the repertoire of IL-12, IL-23 and IL-35 molecules in these species. The multiple p35 genes in teleost fish appear to have arisen via whole genome duplications. The different paralogues of the subunits are divergent, and differentially expressed and modulated by PAMPs and proinflammatory cytokines, hinting that distinct isoforms could be produced in response to infection. Therefore, the expanded IL-12 cytokine family may provide an unprecedented level of regulation to fine tune the immune response in fish. (C) 2014 Elsevier Ltd. All rights reserved.

AB - Interleukin (IL)-12 family cytokines are heterodimers of an alpha-chain (p19, p28 and p35) and a beta-chain (p40 and Ebi3), present as IL-12 (p35/p40), IL-23 (p19/p40), IL-27 (p28/Ebi3) and IL-35 (p35/Ebi3), and play key roles in immune responses in mammals. One p35 and up to three p40 genes have been cloned in some fish species. The identification of three active p35 genes, along with three p40 paralogues in salmonids in the current study further expands the repertoire of IL-12, IL-23 and IL-35 molecules in these species. The multiple p35 genes in teleost fish appear to have arisen via whole genome duplications. The different paralogues of the subunits are divergent, and differentially expressed and modulated by PAMPs and proinflammatory cytokines, hinting that distinct isoforms could be produced in response to infection. Therefore, the expanded IL-12 cytokine family may provide an unprecedented level of regulation to fine tune the immune response in fish. (C) 2014 Elsevier Ltd. All rights reserved.

KW - IL-12

KW - IL-23

KW - IL-35

KW - p35

KW - p40

KW - Salmonid

KW - trout oncorhynchus-mykiss

KW - regulatory mechanisms

KW - genome duplication

KW - adaptive immunity

KW - interleukin-12

KW - bioactivity

KW - cloning

KW - protein

KW - innate

KW - infection

U2 - 10.1016/j.dci.2014.04.008

DO - 10.1016/j.dci.2014.04.008

M3 - Article

VL - 46

SP - 194

EP - 207

JO - Developmental and Comparative Immunology

JF - Developmental and Comparative Immunology

SN - 0145-305X

IS - 2

ER -