The expanding repertoire of the IL-12 cytokine family in teleost fish: Identification of three paralogues each of the p35 and p40 genes in salmonids, and comparative analysis of their expression and modulation in Atlantic salmon Salmo salar

Tiehui Wang; Mansourah Husain

doi:10.1016/j.dci.2014.04.008

The expanding repertoire of the IL-12 cytokine family in teleost fish: Identification of three paralogues each of the p35 and p40 genes in salmonids, and comparative analysis of their expression and modulation in Atlantic salmon Salmo salar

Tiehui Wang^*, Mansourah Husain

^*Corresponding author for this work

University of Aberdeen

Research output: Contribution to journal › Article › peer-review

65 Citations (Scopus)

Abstract

Interleukin (IL)-12 family cytokines are heterodimers of an alpha-chain (p19, p28 and p35) and a beta-chain (p40 and Ebi3), present as IL-12 (p35/p40), IL-23 (p19/p40), IL-27 (p28/Ebi3) and IL-35 (p35/Ebi3), and play key roles in immune responses in mammals. One p35 and up to three p40 genes have been cloned in some fish species. The identification of three active p35 genes, along with three p40 paralogues in salmonids in the current study further expands the repertoire of IL-12, IL-23 and IL-35 molecules in these species. The multiple p35 genes in teleost fish appear to have arisen via whole genome duplications. The different paralogues of the subunits are divergent, and differentially expressed and modulated by PAMPs and proinflammatory cytokines, hinting that distinct isoforms could be produced in response to infection. Therefore, the expanded IL-12 cytokine family may provide an unprecedented level of regulation to fine tune the immune response in fish. (C) 2014 Elsevier Ltd. All rights reserved.

Original language	English
Pages (from-to)	194-207
Number of pages	14
Journal	Developmental and Comparative Immunology
Volume	46
Issue number	2
Early online date	20 Apr 2014
DOIs	https://doi.org/10.1016/j.dci.2014.04.008
Publication status	Published - Oct 2014

Bibliographical note

TW received funding from the MASTS pooling initiative (The Marine Alliance for Science and Technology for Scotland, Scottish Funding Council Grant no. HR09011). M.H. was financially supported by the Public Authority of Applied Education and Training, Kuwait. We thank Prof. C.J. Secombes (University of Aberdeen) for his support and critical reading of this manuscript.

Keywords

IL-12
IL-23
IL-35
p35
p40
Salmonid
trout oncorhynchus-mykiss
regulatory mechanisms
genome duplication
adaptive immunity
interleukin-12
bioactivity
cloning
protein
innate
infection

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Wang, T., & Husain, M. (2014). The expanding repertoire of the IL-12 cytokine family in teleost fish: Identification of three paralogues each of the p35 and p40 genes in salmonids, and comparative analysis of their expression and modulation in Atlantic salmon Salmo salar. Developmental and Comparative Immunology, 46(2), 194-207. https://doi.org/10.1016/j.dci.2014.04.008

The expanding repertoire of the IL-12 cytokine family in teleost fish: Identification of three paralogues each of the p35 and p40 genes in salmonids, and comparative analysis of their expression and modulation in Atlantic salmon Salmo salar. / Wang, Tiehui; Husain, Mansourah.
In: Developmental and Comparative Immunology, Vol. 46, No. 2, 10.2014, p. 194-207.

Research output: Contribution to journal › Article › peer-review

Wang, T & Husain, M 2014, 'The expanding repertoire of the IL-12 cytokine family in teleost fish: Identification of three paralogues each of the p35 and p40 genes in salmonids, and comparative analysis of their expression and modulation in Atlantic salmon Salmo salar', Developmental and Comparative Immunology, vol. 46, no. 2, pp. 194-207. https://doi.org/10.1016/j.dci.2014.04.008

Wang T, Husain M. The expanding repertoire of the IL-12 cytokine family in teleost fish: Identification of three paralogues each of the p35 and p40 genes in salmonids, and comparative analysis of their expression and modulation in Atlantic salmon Salmo salar. Developmental and Comparative Immunology. 2014 Oct;46(2):194-207. Epub 2014 Apr 20. doi: 10.1016/j.dci.2014.04.008

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abstract = "Interleukin (IL)-12 family cytokines are heterodimers of an alpha-chain (p19, p28 and p35) and a beta-chain (p40 and Ebi3), present as IL-12 (p35/p40), IL-23 (p19/p40), IL-27 (p28/Ebi3) and IL-35 (p35/Ebi3), and play key roles in immune responses in mammals. One p35 and up to three p40 genes have been cloned in some fish species. The identification of three active p35 genes, along with three p40 paralogues in salmonids in the current study further expands the repertoire of IL-12, IL-23 and IL-35 molecules in these species. The multiple p35 genes in teleost fish appear to have arisen via whole genome duplications. The different paralogues of the subunits are divergent, and differentially expressed and modulated by PAMPs and proinflammatory cytokines, hinting that distinct isoforms could be produced in response to infection. Therefore, the expanded IL-12 cytokine family may provide an unprecedented level of regulation to fine tune the immune response in fish. (C) 2014 Elsevier Ltd. All rights reserved.",

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note = "TW received funding from the MASTS pooling initiative (The Marine Alliance for Science and Technology for Scotland, Scottish Funding Council Grant no. HR09011). M.H. was financially supported by the Public Authority of Applied Education and Training, Kuwait. We thank Prof. C.J. Secombes (University of Aberdeen) for his support and critical reading of this manuscript.",

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