The expression and secretion of atrial natriuretic factor and brain natriuretic peptide by rat proximal tubular cells

S Mistry, B Lussert, K Stewart, G M Hawksworth, A Struthers, J S McLay

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We examined the expression of both the natriuretic peptides and natriuretic peptide receptors (NPR) in primary cultures of rat proximal tubular (Rm) cells using Northern blot assay fur peptides and receptors and radioimmunoassay and immunohistochemical analysis for atrial natriuretic factor (ANF), brain natriuretic peptide (BNP), and C-type natriuretic peptide. Freshly isolated cells expressed mRNA coding for ANF, BNP, and the NPR-C. The presence of ANF and BNP in freshly isolated cells was confirmed by immunocytochemical staining. As cells approached confluence, there was a marked increase in mRNA expression for ANF and BNP. Immunocytochemical analysis and radioimmunoassay confirmed that both these peptides were co-localised in RPT cells and present in the cell supernatant. These changes in peptide expression were associated with a concurrent decrease in the expression of the NPR-C and the appearance of the NPR-A and -B. These results confirm that freshly isolated RIFT cells possess the components of an autocrine natriuretic peptide system and that growth in primary culture is associated with changes in both peptide system and that growth in primary culture is associated with changes in both peptide and receptor subtype expression, raising the possibility that the endogenous production of ANF and BNP may be involved in the control of control cell growth. BIOCHEM PHARMACOL 59;7:783-790, 2000. (C) 2000 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)783-790
Number of pages8
JournalBiochemical Pharmacology
Volume59
Publication statusPublished - 2000

Keywords

  • rat proximal tubule
  • aerial natriuretic factor
  • brain natriuretic peptide
  • cell growth
  • natriuretic peptide receptor
  • RECEPTOR MESSENGER-RNAS
  • C-RECEPTOR
  • NEPHRON SEGMENTS
  • HUMAN KIDNEY
  • LOCALIZATION
  • PROLIFERATION
  • INHIBITION
  • PROTEIN
  • CULTURE
  • DNA

Cite this

The expression and secretion of atrial natriuretic factor and brain natriuretic peptide by rat proximal tubular cells. / Mistry, S ; Lussert, B ; Stewart, K ; Hawksworth, G M ; Struthers, A ; McLay, J S .

In: Biochemical Pharmacology, Vol. 59, 2000, p. 783-790.

Research output: Contribution to journalArticle

Mistry, S ; Lussert, B ; Stewart, K ; Hawksworth, G M ; Struthers, A ; McLay, J S . / The expression and secretion of atrial natriuretic factor and brain natriuretic peptide by rat proximal tubular cells. In: Biochemical Pharmacology. 2000 ; Vol. 59. pp. 783-790.
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T1 - The expression and secretion of atrial natriuretic factor and brain natriuretic peptide by rat proximal tubular cells

AU - Mistry, S

AU - Lussert, B

AU - Stewart, K

AU - Hawksworth, G M

AU - Struthers, A

AU - McLay, J S

PY - 2000

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N2 - We examined the expression of both the natriuretic peptides and natriuretic peptide receptors (NPR) in primary cultures of rat proximal tubular (Rm) cells using Northern blot assay fur peptides and receptors and radioimmunoassay and immunohistochemical analysis for atrial natriuretic factor (ANF), brain natriuretic peptide (BNP), and C-type natriuretic peptide. Freshly isolated cells expressed mRNA coding for ANF, BNP, and the NPR-C. The presence of ANF and BNP in freshly isolated cells was confirmed by immunocytochemical staining. As cells approached confluence, there was a marked increase in mRNA expression for ANF and BNP. Immunocytochemical analysis and radioimmunoassay confirmed that both these peptides were co-localised in RPT cells and present in the cell supernatant. These changes in peptide expression were associated with a concurrent decrease in the expression of the NPR-C and the appearance of the NPR-A and -B. These results confirm that freshly isolated RIFT cells possess the components of an autocrine natriuretic peptide system and that growth in primary culture is associated with changes in both peptide system and that growth in primary culture is associated with changes in both peptide and receptor subtype expression, raising the possibility that the endogenous production of ANF and BNP may be involved in the control of control cell growth. BIOCHEM PHARMACOL 59;7:783-790, 2000. (C) 2000 Elsevier Science Inc.

AB - We examined the expression of both the natriuretic peptides and natriuretic peptide receptors (NPR) in primary cultures of rat proximal tubular (Rm) cells using Northern blot assay fur peptides and receptors and radioimmunoassay and immunohistochemical analysis for atrial natriuretic factor (ANF), brain natriuretic peptide (BNP), and C-type natriuretic peptide. Freshly isolated cells expressed mRNA coding for ANF, BNP, and the NPR-C. The presence of ANF and BNP in freshly isolated cells was confirmed by immunocytochemical staining. As cells approached confluence, there was a marked increase in mRNA expression for ANF and BNP. Immunocytochemical analysis and radioimmunoassay confirmed that both these peptides were co-localised in RPT cells and present in the cell supernatant. These changes in peptide expression were associated with a concurrent decrease in the expression of the NPR-C and the appearance of the NPR-A and -B. These results confirm that freshly isolated RIFT cells possess the components of an autocrine natriuretic peptide system and that growth in primary culture is associated with changes in both peptide system and that growth in primary culture is associated with changes in both peptide and receptor subtype expression, raising the possibility that the endogenous production of ANF and BNP may be involved in the control of control cell growth. BIOCHEM PHARMACOL 59;7:783-790, 2000. (C) 2000 Elsevier Science Inc.

KW - rat proximal tubule

KW - aerial natriuretic factor

KW - brain natriuretic peptide

KW - cell growth

KW - natriuretic peptide receptor

KW - RECEPTOR MESSENGER-RNAS

KW - C-RECEPTOR

KW - NEPHRON SEGMENTS

KW - HUMAN KIDNEY

KW - LOCALIZATION

KW - PROLIFERATION

KW - INHIBITION

KW - PROTEIN

KW - CULTURE

KW - DNA

M3 - Article

VL - 59

SP - 783

EP - 790

JO - Biochemical Pharmacology

JF - Biochemical Pharmacology

SN - 0006-2952

ER -