The expression of beta 1 integrins in human coronary artery

G S Hillis, R A Mlynski, J G Simpson, A M MacLeod

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    The beta 1 integrin adhesion receptors mediate the binding of cells to extracellular matrices, facilitating their growth, migration, and capacity to deposit matrix proteins: important factors in arterial restenosis and atherosclerosis. The expression of integrins in human coronary artery is, however, unexplored. The aim of the current study was, therefore, to define the expression of beta 1 integrins by cultured human coronary artery vascular smooth muscle cells (hCAVSMC) and in normal human coronary artery; confirming whether or not this differs from the repertoire found in other species and human vessels. The expression of beta 1 integrins by hCAVSMC was assessed by immuno-precipitation and the alkaline phosphatase anti-alkaline phosphatase (APAAP) immunochemical technique. In addition, mRNA expression was defined by reverse transcription polymerase chain reaction (RT-PCR). Normal adult human coronary arteries (n = 4) were also stained by the APAAP method. In vitro hCAVSMC express alpha 2 beta 1 (a collagen and occasional laminin receptor) and alpha 5 beta 1 (a fibronectin receptor) with lesser expression of alpha 3 beta 1 (a multifunctional receptor). They do, however, possess mRNA for several other integrins. Cells within the media of human coronary artery wall express alpha 3 beta 1 and alpha 5 beta 1 but not alpha 2 beta 1: instead the alternative collagen/laminin receptor, alpha 1 beta 1, is expressed in vivo. This pattern of expression differs subtly from that described in rats though it closely parallels that found in other human arteries.

    Original languageEnglish
    Pages (from-to)295-302
    Number of pages8
    JournalBasic Research in Cardiology
    Volume93
    Issue number4
    Publication statusPublished - Aug 1998

    Keywords

    • integrins
    • coronary artery
    • vascular smooth muscle cells
    • SMOOTH-MUSCLE CELLS
    • BIOCHEMICAL-CHARACTERIZATION
    • RECEPTORS
    • ADHESION
    • ATHEROSCLEROSIS
    • HETERODIMERS
    • FIBRONECTIN
    • MEMBRANES
    • PLATELETS
    • COLLAGEN

    Cite this

    Hillis, G. S., Mlynski, R. A., Simpson, J. G., & MacLeod, A. M. (1998). The expression of beta 1 integrins in human coronary artery. Basic Research in Cardiology, 93(4), 295-302.

    The expression of beta 1 integrins in human coronary artery. / Hillis, G S ; Mlynski, R A ; Simpson, J G ; MacLeod, A M .

    In: Basic Research in Cardiology, Vol. 93, No. 4, 08.1998, p. 295-302.

    Research output: Contribution to journalArticle

    Hillis, GS, Mlynski, RA, Simpson, JG & MacLeod, AM 1998, 'The expression of beta 1 integrins in human coronary artery', Basic Research in Cardiology, vol. 93, no. 4, pp. 295-302.
    Hillis GS, Mlynski RA, Simpson JG, MacLeod AM. The expression of beta 1 integrins in human coronary artery. Basic Research in Cardiology. 1998 Aug;93(4):295-302.
    Hillis, G S ; Mlynski, R A ; Simpson, J G ; MacLeod, A M . / The expression of beta 1 integrins in human coronary artery. In: Basic Research in Cardiology. 1998 ; Vol. 93, No. 4. pp. 295-302.
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    AB - The beta 1 integrin adhesion receptors mediate the binding of cells to extracellular matrices, facilitating their growth, migration, and capacity to deposit matrix proteins: important factors in arterial restenosis and atherosclerosis. The expression of integrins in human coronary artery is, however, unexplored. The aim of the current study was, therefore, to define the expression of beta 1 integrins by cultured human coronary artery vascular smooth muscle cells (hCAVSMC) and in normal human coronary artery; confirming whether or not this differs from the repertoire found in other species and human vessels. The expression of beta 1 integrins by hCAVSMC was assessed by immuno-precipitation and the alkaline phosphatase anti-alkaline phosphatase (APAAP) immunochemical technique. In addition, mRNA expression was defined by reverse transcription polymerase chain reaction (RT-PCR). Normal adult human coronary arteries (n = 4) were also stained by the APAAP method. In vitro hCAVSMC express alpha 2 beta 1 (a collagen and occasional laminin receptor) and alpha 5 beta 1 (a fibronectin receptor) with lesser expression of alpha 3 beta 1 (a multifunctional receptor). They do, however, possess mRNA for several other integrins. Cells within the media of human coronary artery wall express alpha 3 beta 1 and alpha 5 beta 1 but not alpha 2 beta 1: instead the alternative collagen/laminin receptor, alpha 1 beta 1, is expressed in vivo. This pattern of expression differs subtly from that described in rats though it closely parallels that found in other human arteries.

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