The FANCM ortholog Fml1 promotes recombination at stalled replication forks and limits crossing over during DNA double-strand break repair

W. Sun, S. Nandi, F. Osman, J.S. Ahn, J. Jakovleska, A. Lorenz, M.C. Whitby

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

The Fanconi anemia (FA) core complex promotes the tolerance/repair of DNA damage at stalled replication forks by catalyzing the monoubiquitination of FANCD2 and FANCI. Intriguingly, the core complex component FANCM also catalyzes branch migration of model Holliday junctions and replication forks in vitro. Here we have characterized the ortholog of FANCM in fission yeast Fml1 in order to understand the physiological significance of this activity. We show that Fml1 has at least two roles in homologous recombination-it promotes Rad51-dependent gene conversion at stalled/blocked replication forks and limits crossing over during mitotic double-strand break repair. In vitro Fml1 catalyzes both replication fork reversal and D loop disruption, indicating possible mechanisms by which it can fulfill its pro- and antirecombinogenic roles.
Original languageEnglish
Pages (from-to)118-128
Number of pages11
JournalMolecular Cell
Volume32
Issue number1
DOIs
Publication statusPublished - 10 Oct 2008

Fingerprint

Cruciform DNA
Fanconi Anemia
Gene Conversion
Double-Stranded DNA Breaks
Schizosaccharomyces
Homologous Recombination
Genetic Recombination
DNA Damage
In Vitro Techniques

Keywords

  • DNA

Cite this

The FANCM ortholog Fml1 promotes recombination at stalled replication forks and limits crossing over during DNA double-strand break repair. / Sun, W.; Nandi, S.; Osman, F.; Ahn, J.S.; Jakovleska, J.; Lorenz, A.; Whitby, M.C.

In: Molecular Cell, Vol. 32, No. 1, 10.10.2008, p. 118-128.

Research output: Contribution to journalArticle

Sun, W. ; Nandi, S. ; Osman, F. ; Ahn, J.S. ; Jakovleska, J. ; Lorenz, A. ; Whitby, M.C. / The FANCM ortholog Fml1 promotes recombination at stalled replication forks and limits crossing over during DNA double-strand break repair. In: Molecular Cell. 2008 ; Vol. 32, No. 1. pp. 118-128.
@article{12f67b0456454b7e8ff2aa19228b1312,
title = "The FANCM ortholog Fml1 promotes recombination at stalled replication forks and limits crossing over during DNA double-strand break repair",
abstract = "The Fanconi anemia (FA) core complex promotes the tolerance/repair of DNA damage at stalled replication forks by catalyzing the monoubiquitination of FANCD2 and FANCI. Intriguingly, the core complex component FANCM also catalyzes branch migration of model Holliday junctions and replication forks in vitro. Here we have characterized the ortholog of FANCM in fission yeast Fml1 in order to understand the physiological significance of this activity. We show that Fml1 has at least two roles in homologous recombination-it promotes Rad51-dependent gene conversion at stalled/blocked replication forks and limits crossing over during mitotic double-strand break repair. In vitro Fml1 catalyzes both replication fork reversal and D loop disruption, indicating possible mechanisms by which it can fulfill its pro- and antirecombinogenic roles.",
keywords = "DNA",
author = "W. Sun and S. Nandi and F. Osman and J.S. Ahn and J. Jakovleska and A. Lorenz and M.C. Whitby",
year = "2008",
month = "10",
day = "10",
doi = "10.1016/j.molcel.2008.08.024",
language = "English",
volume = "32",
pages = "118--128",
journal = "Molecular Cell",
issn = "1097-2765",
publisher = "Cell Press",
number = "1",

}

TY - JOUR

T1 - The FANCM ortholog Fml1 promotes recombination at stalled replication forks and limits crossing over during DNA double-strand break repair

AU - Sun, W.

AU - Nandi, S.

AU - Osman, F.

AU - Ahn, J.S.

AU - Jakovleska, J.

AU - Lorenz, A.

AU - Whitby, M.C.

PY - 2008/10/10

Y1 - 2008/10/10

N2 - The Fanconi anemia (FA) core complex promotes the tolerance/repair of DNA damage at stalled replication forks by catalyzing the monoubiquitination of FANCD2 and FANCI. Intriguingly, the core complex component FANCM also catalyzes branch migration of model Holliday junctions and replication forks in vitro. Here we have characterized the ortholog of FANCM in fission yeast Fml1 in order to understand the physiological significance of this activity. We show that Fml1 has at least two roles in homologous recombination-it promotes Rad51-dependent gene conversion at stalled/blocked replication forks and limits crossing over during mitotic double-strand break repair. In vitro Fml1 catalyzes both replication fork reversal and D loop disruption, indicating possible mechanisms by which it can fulfill its pro- and antirecombinogenic roles.

AB - The Fanconi anemia (FA) core complex promotes the tolerance/repair of DNA damage at stalled replication forks by catalyzing the monoubiquitination of FANCD2 and FANCI. Intriguingly, the core complex component FANCM also catalyzes branch migration of model Holliday junctions and replication forks in vitro. Here we have characterized the ortholog of FANCM in fission yeast Fml1 in order to understand the physiological significance of this activity. We show that Fml1 has at least two roles in homologous recombination-it promotes Rad51-dependent gene conversion at stalled/blocked replication forks and limits crossing over during mitotic double-strand break repair. In vitro Fml1 catalyzes both replication fork reversal and D loop disruption, indicating possible mechanisms by which it can fulfill its pro- and antirecombinogenic roles.

KW - DNA

UR - http://www.scopus.com/inward/record.url?scp=53149087431&partnerID=8YFLogxK

U2 - 10.1016/j.molcel.2008.08.024

DO - 10.1016/j.molcel.2008.08.024

M3 - Article

VL - 32

SP - 118

EP - 128

JO - Molecular Cell

JF - Molecular Cell

SN - 1097-2765

IS - 1

ER -