The fate of Escherichia coli and E-coli O157 in cattle slurry after application to land

D R Fenlon, I D Ogden, A Vinten, I Svoboda

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

The fate of both faecal Escherichia coli and E. coli O157 in slurry following application to arable and grass plots on a clay loam soil was studied. Slurry (5% dry matter) containing 5.3 x 10(4) ml(-1) E. coli and 30 E. coli O157 100 ml(-1) was spread in early March. Initially, almost all E. coli were retained in the upper layers of the soil. Escherichia coli numbers steadily declined to less than 1% of those applied by day 29, and E. coli O157 were only detected in the soil and on the grass for the first week after application. There was some transport of bacteria to deeper layers of the soil, but this was approximately 2% of the total; transport to drains over the same period was mainly associated with rainfall events and amounted to approximately 7% of applied E. coli. However, there were indications that periods of heavy rainfall could cause significant losses of E. coli by both leaching and run-off. Experimental studies showed that E. coli O157 on grass, which was subsequently ensiled in conditions allowing aerobic spoilage, could multiply to numbers exceeding 10(6) g(-1) in the silage.

Original languageEnglish
Number of pages8
JournalJournal of Applied Microbiology
Volume88
Publication statusPublished - 2000

Keywords

  • GRASS
  • LISTERIA
  • ENTEROBACTERIA
  • NITRATE
  • GROWTH
  • SILAGE
  • FARMS

Cite this

The fate of Escherichia coli and E-coli O157 in cattle slurry after application to land. / Fenlon, D R ; Ogden, I D ; Vinten, A ; Svoboda, I .

In: Journal of Applied Microbiology, Vol. 88, 2000.

Research output: Contribution to journalArticle

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abstract = "The fate of both faecal Escherichia coli and E. coli O157 in slurry following application to arable and grass plots on a clay loam soil was studied. Slurry (5{\%} dry matter) containing 5.3 x 10(4) ml(-1) E. coli and 30 E. coli O157 100 ml(-1) was spread in early March. Initially, almost all E. coli were retained in the upper layers of the soil. Escherichia coli numbers steadily declined to less than 1{\%} of those applied by day 29, and E. coli O157 were only detected in the soil and on the grass for the first week after application. There was some transport of bacteria to deeper layers of the soil, but this was approximately 2{\%} of the total; transport to drains over the same period was mainly associated with rainfall events and amounted to approximately 7{\%} of applied E. coli. However, there were indications that periods of heavy rainfall could cause significant losses of E. coli by both leaching and run-off. Experimental studies showed that E. coli O157 on grass, which was subsequently ensiled in conditions allowing aerobic spoilage, could multiply to numbers exceeding 10(6) g(-1) in the silage.",
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TY - JOUR

T1 - The fate of Escherichia coli and E-coli O157 in cattle slurry after application to land

AU - Fenlon, D R

AU - Ogden, I D

AU - Vinten, A

AU - Svoboda, I

PY - 2000

Y1 - 2000

N2 - The fate of both faecal Escherichia coli and E. coli O157 in slurry following application to arable and grass plots on a clay loam soil was studied. Slurry (5% dry matter) containing 5.3 x 10(4) ml(-1) E. coli and 30 E. coli O157 100 ml(-1) was spread in early March. Initially, almost all E. coli were retained in the upper layers of the soil. Escherichia coli numbers steadily declined to less than 1% of those applied by day 29, and E. coli O157 were only detected in the soil and on the grass for the first week after application. There was some transport of bacteria to deeper layers of the soil, but this was approximately 2% of the total; transport to drains over the same period was mainly associated with rainfall events and amounted to approximately 7% of applied E. coli. However, there were indications that periods of heavy rainfall could cause significant losses of E. coli by both leaching and run-off. Experimental studies showed that E. coli O157 on grass, which was subsequently ensiled in conditions allowing aerobic spoilage, could multiply to numbers exceeding 10(6) g(-1) in the silage.

AB - The fate of both faecal Escherichia coli and E. coli O157 in slurry following application to arable and grass plots on a clay loam soil was studied. Slurry (5% dry matter) containing 5.3 x 10(4) ml(-1) E. coli and 30 E. coli O157 100 ml(-1) was spread in early March. Initially, almost all E. coli were retained in the upper layers of the soil. Escherichia coli numbers steadily declined to less than 1% of those applied by day 29, and E. coli O157 were only detected in the soil and on the grass for the first week after application. There was some transport of bacteria to deeper layers of the soil, but this was approximately 2% of the total; transport to drains over the same period was mainly associated with rainfall events and amounted to approximately 7% of applied E. coli. However, there were indications that periods of heavy rainfall could cause significant losses of E. coli by both leaching and run-off. Experimental studies showed that E. coli O157 on grass, which was subsequently ensiled in conditions allowing aerobic spoilage, could multiply to numbers exceeding 10(6) g(-1) in the silage.

KW - GRASS

KW - LISTERIA

KW - ENTEROBACTERIA

KW - NITRATE

KW - GROWTH

KW - SILAGE

KW - FARMS

M3 - Article

VL - 88

JO - Journal of Applied Microbiology

JF - Journal of Applied Microbiology

SN - 1364-5072

ER -