The fetal ovary exhibits temporal sensitivity to a ‘real-life’ mixture of environmental chemicals

Richard G. Lea, Maria R. Amezaga, Benoit Loup, Beatrice Mandon-Pepin, Agnes Stefansdottir, Panagiotis Filis, Carol Kyle, Zulin Zhang, Ceri Allen, Laura Purdie, Luc Jouneau, Corinne Cotinot, Stewart M. Rhind, Kevin D. Sinclair, Paul A. Fowler

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Abstract

The development of fetal ovarian follicles is a critical determinant of adult female reproductive competence. Prolonged exposure to environmental chemicals (ECs) can perturb this process with detrimental consequences for offspring. Here we report on the exposure of pregnant ewes to an environmental mixture of ECs derived from pastures fertilized with sewage sludge (biosolids): a common global agricultural practice. Exposure of pregnant ewes to ECs over 80 day periods during early, mid or late gestation reduced the proportion of healthy early stage fetal follicles comprising the ovarian reserve. Mid and late gestation EC exposures had the most marked effects, disturbing maternal and fetal liver chemical profiles, masculinising fetal anogenital distance and greatly increasing the number of altered fetal ovarian genes and proteins. In conclusion, differential temporal sensitivity of the fetus and its ovaries to EC mixtures has implications for adult ovarian function following adverse exposures during pregnancy.
Original languageEnglish
Article number22279
Pages (from-to)1-13
Number of pages13
JournalScientific Reports
Volume6
Early online date2 Mar 2016
DOIs
Publication statusPublished - 2 Mar 2016

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Ovary
Environmental Exposure
Sewage
Pregnancy
Ovarian Follicle
Fetal Development
Mental Competency
Fetus
Mothers
Liver
Proteins
Ovarian Reserve

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The fetal ovary exhibits temporal sensitivity to a ‘real-life’ mixture of environmental chemicals. / Lea, Richard G.; Amezaga, Maria R.; Loup, Benoit; Mandon-Pepin, Beatrice; Stefansdottir, Agnes; Filis, Panagiotis; Kyle, Carol; Zhang, Zulin ; Allen, Ceri ; Purdie, Laura ; Jouneau, Luc; Cotinot, Corinne; Rhind, Stewart M.; Sinclair, Kevin D.; Fowler, Paul A.

In: Scientific Reports, Vol. 6, 22279, 02.03.2016, p. 1-13.

Research output: Contribution to journalArticle

Lea, RG, Amezaga, MR, Loup, B, Mandon-Pepin, B, Stefansdottir, A, Filis, P, Kyle, C, Zhang, Z, Allen, C, Purdie, L, Jouneau, L, Cotinot, C, Rhind, SM, Sinclair, KD & Fowler, PA 2016, 'The fetal ovary exhibits temporal sensitivity to a ‘real-life’ mixture of environmental chemicals', Scientific Reports, vol. 6, 22279, pp. 1-13. https://doi.org/10.1038/srep22279
Lea, Richard G. ; Amezaga, Maria R. ; Loup, Benoit ; Mandon-Pepin, Beatrice ; Stefansdottir, Agnes ; Filis, Panagiotis ; Kyle, Carol ; Zhang, Zulin ; Allen, Ceri ; Purdie, Laura ; Jouneau, Luc ; Cotinot, Corinne ; Rhind, Stewart M. ; Sinclair, Kevin D. ; Fowler, Paul A. / The fetal ovary exhibits temporal sensitivity to a ‘real-life’ mixture of environmental chemicals. In: Scientific Reports. 2016 ; Vol. 6. pp. 1-13.
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