The fission yeast FANCM ortholog directs non-crossover recombination during meiosis

A. Lorenz, F. Osman, W. Sun, S. Nandi, R. Steinacher, M.C. Whitby

Research output: Contribution to journalArticle

55 Citations (Scopus)
4 Downloads (Pure)

Abstract

The formation of healthy gametes depends on programmed DNA double-strand breaks (DSBs), which are each repaired as a crossover (CO) or non-crossover (NCO) from a homologous template. Although most of these DSBs are repaired without giving COs, little is known about the genetic requirements of NCO-specific recombination. We show that Fml1, the Fanconi anemia complementation group M (FANCM) - ortholog of Schizosaccharomyces pombe, directs the formation of NCOs during meiosis in competition with the Mus81-dependent pro-CO pathway. We also define the Rad51/Dmc1 - mediator Swi5-Sfr1 as a major determinant in biasing the recombination process in favor of Mus81, to ensure the appropriate amount of COs to guide meiotic chromosome segregation. The conservation of these proteins from yeast to humans suggests that this interplay may be a general feature of meiotic recombination.
Original languageEnglish
Pages (from-to)1585-1588
Number of pages4
JournalScience
Volume336
Issue number6088
DOIs
Publication statusPublished - 22 Jun 2012

Fingerprint

Fanconi Anemia
Schizosaccharomyces
Meiosis
Genetic Recombination
Chromosome Segregation
Fungal Proteins
Double-Stranded DNA Breaks
Germ Cells

Cite this

Lorenz, A., Osman, F., Sun, W., Nandi, S., Steinacher, R., & Whitby, M. C. (2012). The fission yeast FANCM ortholog directs non-crossover recombination during meiosis. Science, 336(6088), 1585-1588. https://doi.org/10.1126/science.1220111

The fission yeast FANCM ortholog directs non-crossover recombination during meiosis. / Lorenz, A.; Osman, F.; Sun, W.; Nandi, S.; Steinacher, R.; Whitby, M.C.

In: Science, Vol. 336, No. 6088, 22.06.2012, p. 1585-1588.

Research output: Contribution to journalArticle

Lorenz, A, Osman, F, Sun, W, Nandi, S, Steinacher, R & Whitby, MC 2012, 'The fission yeast FANCM ortholog directs non-crossover recombination during meiosis', Science, vol. 336, no. 6088, pp. 1585-1588. https://doi.org/10.1126/science.1220111
Lorenz, A. ; Osman, F. ; Sun, W. ; Nandi, S. ; Steinacher, R. ; Whitby, M.C. / The fission yeast FANCM ortholog directs non-crossover recombination during meiosis. In: Science. 2012 ; Vol. 336, No. 6088. pp. 1585-1588.
@article{c439efb59bac4cf1ab55840395ba2e8c,
title = "The fission yeast FANCM ortholog directs non-crossover recombination during meiosis",
abstract = "The formation of healthy gametes depends on programmed DNA double-strand breaks (DSBs), which are each repaired as a crossover (CO) or non-crossover (NCO) from a homologous template. Although most of these DSBs are repaired without giving COs, little is known about the genetic requirements of NCO-specific recombination. We show that Fml1, the Fanconi anemia complementation group M (FANCM) - ortholog of Schizosaccharomyces pombe, directs the formation of NCOs during meiosis in competition with the Mus81-dependent pro-CO pathway. We also define the Rad51/Dmc1 - mediator Swi5-Sfr1 as a major determinant in biasing the recombination process in favor of Mus81, to ensure the appropriate amount of COs to guide meiotic chromosome segregation. The conservation of these proteins from yeast to humans suggests that this interplay may be a general feature of meiotic recombination.",
author = "A. Lorenz and F. Osman and W. Sun and S. Nandi and R. Steinacher and M.C. Whitby",
year = "2012",
month = "6",
day = "22",
doi = "10.1126/science.1220111",
language = "English",
volume = "336",
pages = "1585--1588",
journal = "Science",
issn = "0036-8075",
publisher = "AMER ASSOC ADVANCEMENT SCIENCE",
number = "6088",

}

TY - JOUR

T1 - The fission yeast FANCM ortholog directs non-crossover recombination during meiosis

AU - Lorenz, A.

AU - Osman, F.

AU - Sun, W.

AU - Nandi, S.

AU - Steinacher, R.

AU - Whitby, M.C.

PY - 2012/6/22

Y1 - 2012/6/22

N2 - The formation of healthy gametes depends on programmed DNA double-strand breaks (DSBs), which are each repaired as a crossover (CO) or non-crossover (NCO) from a homologous template. Although most of these DSBs are repaired without giving COs, little is known about the genetic requirements of NCO-specific recombination. We show that Fml1, the Fanconi anemia complementation group M (FANCM) - ortholog of Schizosaccharomyces pombe, directs the formation of NCOs during meiosis in competition with the Mus81-dependent pro-CO pathway. We also define the Rad51/Dmc1 - mediator Swi5-Sfr1 as a major determinant in biasing the recombination process in favor of Mus81, to ensure the appropriate amount of COs to guide meiotic chromosome segregation. The conservation of these proteins from yeast to humans suggests that this interplay may be a general feature of meiotic recombination.

AB - The formation of healthy gametes depends on programmed DNA double-strand breaks (DSBs), which are each repaired as a crossover (CO) or non-crossover (NCO) from a homologous template. Although most of these DSBs are repaired without giving COs, little is known about the genetic requirements of NCO-specific recombination. We show that Fml1, the Fanconi anemia complementation group M (FANCM) - ortholog of Schizosaccharomyces pombe, directs the formation of NCOs during meiosis in competition with the Mus81-dependent pro-CO pathway. We also define the Rad51/Dmc1 - mediator Swi5-Sfr1 as a major determinant in biasing the recombination process in favor of Mus81, to ensure the appropriate amount of COs to guide meiotic chromosome segregation. The conservation of these proteins from yeast to humans suggests that this interplay may be a general feature of meiotic recombination.

UR - http://www.scopus.com/inward/record.url?scp=84862603467&partnerID=8YFLogxK

U2 - 10.1126/science.1220111

DO - 10.1126/science.1220111

M3 - Article

AN - SCOPUS:84862603467

VL - 336

SP - 1585

EP - 1588

JO - Science

JF - Science

SN - 0036-8075

IS - 6088

ER -