The GH/IGF-I axis hormones and bone markers in elite athletes in response to a maximum exercise test

C. Ehrnborg, K. H. Lange, R. Dall, J. S. Christiansen, P. A. Lundberg, R. Baxter, Massoud Boroujerdi, B. A. Bengtsson, M. L. Healey, C. Pentecost, S. Longobardi, R. Napoli, T. Rosen

    Research output: Contribution to journalArticle

    87 Citations (Scopus)

    Abstract

    The aim of the GH-2000 project is to develop a method for detecting GH doping among athletes. Previous papers in the GH-2000 project have proposed that a forthcoming method to detect GH doping will need specific components from the GH/IGF-I axis and bone markers because these specific variables seem more sensitive to exogenous GH than to exercise. The present study examined the responses of the serum concentrations of these specific variables to a maximum exercise test in elite athletes from selected sports. A total of 117 elite athletes (84 males and 33 females; mean age, 25 yr; range, 18-53 yr) from Denmark, the United Kingdom, Italy, and Sweden participated in the study. The serum concentrations of total GH, GH22 kDa, IGF-I, IGF binding protein (IGFBP)-2, IGFBP-3, acid-labile subunit, procollagen type III (P-III-P), and the bone markers osteocalcin, carboxy-terminal crosslinked telopeptide of type I collagen (ICTP), and carboxyterminal propeptide of type I procollagen were measured.

    The maximum exercise test showed, in both genders, a peak concentration of total GH (P < 0.001) and GH22 kDa (P < 0.001) by the time exercise ended compared with baseline, and a subsequent decrease to baseline levels within 30 - 60 min after exercise. The mean time to peak value for total GH and GH22 kDa was significantly shorter in males than females (P < 0.001). The components of the IGF-I axis showed a similar pattern, with a peak value after exercise compared with base-line for IGF-I (P < 0.001, males and females); IGFBP-3 (P < 0.001, males;and females); acid-labile subunit [P < 0.001, males; not significant (NS), females], and IGFBP-2 (P < 0.05, females; NS, males).

    The serum concentrations of the bone markers ICTP (P < 0.001, males; P < 0.05, females) and P-III-P (P < 0.001, males and females) increased in both genders, with a peak value in the direct post-exercise phase and a subsequent decrease to baseline levels or below within 120 min.

    The osteocalcin and propeptide of type I procollagen values did not change during the exercise test. Specific reference ranges for each variable in the GH/IGF-I axis and bone markers at specific time points are presented. Most of the variables correlated negatively with age.

    In summary, the maximum exercise test showed a rather uniform pattern, with peak concentrations of the GH/IGF-I axis hormones and the bone markers ICTP and P-III-P immediately after exercise, followed by a subsequent decrease to baseline levels. The time to peak value for total GH and GH22 kDa was significantly shorter for females compared with males. This paper presents reference ranges for each marker in each gender at specific time points in connection to a maximum exercise test to be used in the development of a test for detection of GH abuse in sports.

    Original languageEnglish
    Pages (from-to)394-401
    Number of pages7
    JournalJournal of Clinical Endocrinology and Metabolism
    Volume88
    Issue number1
    DOIs
    Publication statusPublished - Jan 2003

    Fingerprint

    Insulin-Like Growth Factor I
    Exercise Test
    Athletes
    Bone
    Hormones
    Bone and Bones
    Collagen Type I
    Insulin-Like Growth Factor Binding Protein 2
    Insulin-Like Growth Factor Binding Protein 3
    Osteocalcin
    Sports
    Exercise
    Doping (additives)
    Acids
    Collagen Type III
    Reference Values
    Serum
    Denmark
    Sweden
    Italy

    Keywords

    • trained adult males
    • parathyroid-hormone
    • collagen turnover
    • mineral density
    • GH withdrawal
    • men
    • work
    • osteocalcin
    • metabolism
    • intensity

    Cite this

    Ehrnborg, C., Lange, K. H., Dall, R., Christiansen, J. S., Lundberg, P. A., Baxter, R., ... Rosen, T. (2003). The GH/IGF-I axis hormones and bone markers in elite athletes in response to a maximum exercise test. Journal of Clinical Endocrinology and Metabolism, 88(1), 394-401. https://doi.org/10.1210/jc.2002-020037

    The GH/IGF-I axis hormones and bone markers in elite athletes in response to a maximum exercise test. / Ehrnborg, C.; Lange, K. H.; Dall, R.; Christiansen, J. S.; Lundberg, P. A.; Baxter, R.; Boroujerdi, Massoud; Bengtsson, B. A.; Healey, M. L.; Pentecost, C.; Longobardi, S.; Napoli, R.; Rosen, T.

    In: Journal of Clinical Endocrinology and Metabolism, Vol. 88, No. 1, 01.2003, p. 394-401.

    Research output: Contribution to journalArticle

    Ehrnborg, C, Lange, KH, Dall, R, Christiansen, JS, Lundberg, PA, Baxter, R, Boroujerdi, M, Bengtsson, BA, Healey, ML, Pentecost, C, Longobardi, S, Napoli, R & Rosen, T 2003, 'The GH/IGF-I axis hormones and bone markers in elite athletes in response to a maximum exercise test', Journal of Clinical Endocrinology and Metabolism, vol. 88, no. 1, pp. 394-401. https://doi.org/10.1210/jc.2002-020037
    Ehrnborg, C. ; Lange, K. H. ; Dall, R. ; Christiansen, J. S. ; Lundberg, P. A. ; Baxter, R. ; Boroujerdi, Massoud ; Bengtsson, B. A. ; Healey, M. L. ; Pentecost, C. ; Longobardi, S. ; Napoli, R. ; Rosen, T. / The GH/IGF-I axis hormones and bone markers in elite athletes in response to a maximum exercise test. In: Journal of Clinical Endocrinology and Metabolism. 2003 ; Vol. 88, No. 1. pp. 394-401.
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    AU - Ehrnborg, C.

    AU - Lange, K. H.

    AU - Dall, R.

    AU - Christiansen, J. S.

    AU - Lundberg, P. A.

    AU - Baxter, R.

    AU - Boroujerdi, Massoud

    AU - Bengtsson, B. A.

    AU - Healey, M. L.

    AU - Pentecost, C.

    AU - Longobardi, S.

    AU - Napoli, R.

    AU - Rosen, T.

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    N2 - The aim of the GH-2000 project is to develop a method for detecting GH doping among athletes. Previous papers in the GH-2000 project have proposed that a forthcoming method to detect GH doping will need specific components from the GH/IGF-I axis and bone markers because these specific variables seem more sensitive to exogenous GH than to exercise. The present study examined the responses of the serum concentrations of these specific variables to a maximum exercise test in elite athletes from selected sports. A total of 117 elite athletes (84 males and 33 females; mean age, 25 yr; range, 18-53 yr) from Denmark, the United Kingdom, Italy, and Sweden participated in the study. The serum concentrations of total GH, GH22 kDa, IGF-I, IGF binding protein (IGFBP)-2, IGFBP-3, acid-labile subunit, procollagen type III (P-III-P), and the bone markers osteocalcin, carboxy-terminal crosslinked telopeptide of type I collagen (ICTP), and carboxyterminal propeptide of type I procollagen were measured.The maximum exercise test showed, in both genders, a peak concentration of total GH (P < 0.001) and GH22 kDa (P < 0.001) by the time exercise ended compared with baseline, and a subsequent decrease to baseline levels within 30 - 60 min after exercise. The mean time to peak value for total GH and GH22 kDa was significantly shorter in males than females (P < 0.001). The components of the IGF-I axis showed a similar pattern, with a peak value after exercise compared with base-line for IGF-I (P < 0.001, males and females); IGFBP-3 (P < 0.001, males;and females); acid-labile subunit [P < 0.001, males; not significant (NS), females], and IGFBP-2 (P < 0.05, females; NS, males).The serum concentrations of the bone markers ICTP (P < 0.001, males; P < 0.05, females) and P-III-P (P < 0.001, males and females) increased in both genders, with a peak value in the direct post-exercise phase and a subsequent decrease to baseline levels or below within 120 min.The osteocalcin and propeptide of type I procollagen values did not change during the exercise test. Specific reference ranges for each variable in the GH/IGF-I axis and bone markers at specific time points are presented. Most of the variables correlated negatively with age.In summary, the maximum exercise test showed a rather uniform pattern, with peak concentrations of the GH/IGF-I axis hormones and the bone markers ICTP and P-III-P immediately after exercise, followed by a subsequent decrease to baseline levels. The time to peak value for total GH and GH22 kDa was significantly shorter for females compared with males. This paper presents reference ranges for each marker in each gender at specific time points in connection to a maximum exercise test to be used in the development of a test for detection of GH abuse in sports.

    AB - The aim of the GH-2000 project is to develop a method for detecting GH doping among athletes. Previous papers in the GH-2000 project have proposed that a forthcoming method to detect GH doping will need specific components from the GH/IGF-I axis and bone markers because these specific variables seem more sensitive to exogenous GH than to exercise. The present study examined the responses of the serum concentrations of these specific variables to a maximum exercise test in elite athletes from selected sports. A total of 117 elite athletes (84 males and 33 females; mean age, 25 yr; range, 18-53 yr) from Denmark, the United Kingdom, Italy, and Sweden participated in the study. The serum concentrations of total GH, GH22 kDa, IGF-I, IGF binding protein (IGFBP)-2, IGFBP-3, acid-labile subunit, procollagen type III (P-III-P), and the bone markers osteocalcin, carboxy-terminal crosslinked telopeptide of type I collagen (ICTP), and carboxyterminal propeptide of type I procollagen were measured.The maximum exercise test showed, in both genders, a peak concentration of total GH (P < 0.001) and GH22 kDa (P < 0.001) by the time exercise ended compared with baseline, and a subsequent decrease to baseline levels within 30 - 60 min after exercise. The mean time to peak value for total GH and GH22 kDa was significantly shorter in males than females (P < 0.001). The components of the IGF-I axis showed a similar pattern, with a peak value after exercise compared with base-line for IGF-I (P < 0.001, males and females); IGFBP-3 (P < 0.001, males;and females); acid-labile subunit [P < 0.001, males; not significant (NS), females], and IGFBP-2 (P < 0.05, females; NS, males).The serum concentrations of the bone markers ICTP (P < 0.001, males; P < 0.05, females) and P-III-P (P < 0.001, males and females) increased in both genders, with a peak value in the direct post-exercise phase and a subsequent decrease to baseline levels or below within 120 min.The osteocalcin and propeptide of type I procollagen values did not change during the exercise test. Specific reference ranges for each variable in the GH/IGF-I axis and bone markers at specific time points are presented. Most of the variables correlated negatively with age.In summary, the maximum exercise test showed a rather uniform pattern, with peak concentrations of the GH/IGF-I axis hormones and the bone markers ICTP and P-III-P immediately after exercise, followed by a subsequent decrease to baseline levels. The time to peak value for total GH and GH22 kDa was significantly shorter for females compared with males. This paper presents reference ranges for each marker in each gender at specific time points in connection to a maximum exercise test to be used in the development of a test for detection of GH abuse in sports.

    KW - trained adult males

    KW - parathyroid-hormone

    KW - collagen turnover

    KW - mineral density

    KW - GH withdrawal

    KW - men

    KW - work

    KW - osteocalcin

    KW - metabolism

    KW - intensity

    U2 - 10.1210/jc.2002-020037

    DO - 10.1210/jc.2002-020037

    M3 - Article

    VL - 88

    SP - 394

    EP - 401

    JO - Journal of Clinical Endocrinology and Metabolism

    JF - Journal of Clinical Endocrinology and Metabolism

    SN - 0021-972X

    IS - 1

    ER -