The gills are an important site of iNOS expression in rainbow trout Oncorhynchus mykiss after challenge with the Gram-positive pathogen Renibacterium salmoninarum

J J Campos-Perez, M Ward, P S Grabowski, A E Ellis, C J Secombes

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

Following injection challenge of rainbow trout with the Gram-positive pathogen Renibacterium salmoninarum?, serum nitrate levels increased indicative of NO production. The timing and amount of nitrate produced varied with the virulence of the bacterial strain used, with the highest levels seen in fish challenged with the most virulent (autoaggregating) strain. Immunization with a killed R. salmoninarum preparation in Freund's incomplete adjuvant significantly increased nitrate levels after challenge. Inducible nitric oxide synthase (iNOS) transcript expression was detectable in rainbow trout tissues after injection challenge with R salmoninarum, and its induction in the gills was both quick (between 3 and 6 hr) and relatively prolonged (lasting several days). iNOS expression in the kidney was also seen at a later stage (24 hr) but appeared to switch off relatively rapidly. Bath challenge with R salmoninarum also induced iNOS expression in gill, and a variable expression in the gut and kidney also occurred. These results highlight the importance of the gills, not only as a point of entry of pathogens but also as a tissue capable of mounting an immune response.

Original languageEnglish
Pages (from-to)153-161
Number of pages9
JournalImmunology
Volume99
Publication statusPublished - 2000

Keywords

  • NITRIC-OXIDE SYNTHASE
  • MACROPHAGE ACTIVATING FACTOR(S)
  • SALMO-GAIRDNERI RICHARDSON
  • BACTERIAL KIDNEY-DISEASE
  • ICTALURUS-PUNCTATUS
  • CHANNEL CATFISH
  • ATLANTIC SALMON
  • NERVOUS-SYSTEM
  • L-ARGININE
  • CELL-LINE

Cite this

The gills are an important site of iNOS expression in rainbow trout Oncorhynchus mykiss after challenge with the Gram-positive pathogen Renibacterium salmoninarum. / Campos-Perez, J J ; Ward, M ; Grabowski, P S ; Ellis, A E ; Secombes, C J .

In: Immunology, Vol. 99, 2000, p. 153-161.

Research output: Contribution to journalArticle

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abstract = "Following injection challenge of rainbow trout with the Gram-positive pathogen Renibacterium salmoninarum?, serum nitrate levels increased indicative of NO production. The timing and amount of nitrate produced varied with the virulence of the bacterial strain used, with the highest levels seen in fish challenged with the most virulent (autoaggregating) strain. Immunization with a killed R. salmoninarum preparation in Freund's incomplete adjuvant significantly increased nitrate levels after challenge. Inducible nitric oxide synthase (iNOS) transcript expression was detectable in rainbow trout tissues after injection challenge with R salmoninarum, and its induction in the gills was both quick (between 3 and 6 hr) and relatively prolonged (lasting several days). iNOS expression in the kidney was also seen at a later stage (24 hr) but appeared to switch off relatively rapidly. Bath challenge with R salmoninarum also induced iNOS expression in gill, and a variable expression in the gut and kidney also occurred. These results highlight the importance of the gills, not only as a point of entry of pathogens but also as a tissue capable of mounting an immune response.",
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T1 - The gills are an important site of iNOS expression in rainbow trout Oncorhynchus mykiss after challenge with the Gram-positive pathogen Renibacterium salmoninarum

AU - Campos-Perez, J J

AU - Ward, M

AU - Grabowski, P S

AU - Ellis, A E

AU - Secombes, C J

PY - 2000

Y1 - 2000

N2 - Following injection challenge of rainbow trout with the Gram-positive pathogen Renibacterium salmoninarum?, serum nitrate levels increased indicative of NO production. The timing and amount of nitrate produced varied with the virulence of the bacterial strain used, with the highest levels seen in fish challenged with the most virulent (autoaggregating) strain. Immunization with a killed R. salmoninarum preparation in Freund's incomplete adjuvant significantly increased nitrate levels after challenge. Inducible nitric oxide synthase (iNOS) transcript expression was detectable in rainbow trout tissues after injection challenge with R salmoninarum, and its induction in the gills was both quick (between 3 and 6 hr) and relatively prolonged (lasting several days). iNOS expression in the kidney was also seen at a later stage (24 hr) but appeared to switch off relatively rapidly. Bath challenge with R salmoninarum also induced iNOS expression in gill, and a variable expression in the gut and kidney also occurred. These results highlight the importance of the gills, not only as a point of entry of pathogens but also as a tissue capable of mounting an immune response.

AB - Following injection challenge of rainbow trout with the Gram-positive pathogen Renibacterium salmoninarum?, serum nitrate levels increased indicative of NO production. The timing and amount of nitrate produced varied with the virulence of the bacterial strain used, with the highest levels seen in fish challenged with the most virulent (autoaggregating) strain. Immunization with a killed R. salmoninarum preparation in Freund's incomplete adjuvant significantly increased nitrate levels after challenge. Inducible nitric oxide synthase (iNOS) transcript expression was detectable in rainbow trout tissues after injection challenge with R salmoninarum, and its induction in the gills was both quick (between 3 and 6 hr) and relatively prolonged (lasting several days). iNOS expression in the kidney was also seen at a later stage (24 hr) but appeared to switch off relatively rapidly. Bath challenge with R salmoninarum also induced iNOS expression in gill, and a variable expression in the gut and kidney also occurred. These results highlight the importance of the gills, not only as a point of entry of pathogens but also as a tissue capable of mounting an immune response.

KW - NITRIC-OXIDE SYNTHASE

KW - MACROPHAGE ACTIVATING FACTOR(S)

KW - SALMO-GAIRDNERI RICHARDSON

KW - BACTERIAL KIDNEY-DISEASE

KW - ICTALURUS-PUNCTATUS

KW - CHANNEL CATFISH

KW - ATLANTIC SALMON

KW - NERVOUS-SYSTEM

KW - L-ARGININE

KW - CELL-LINE

M3 - Article

VL - 99

SP - 153

EP - 161

JO - Immunology

JF - Immunology

SN - 0019-2805

ER -