The gut anaerobe Faecalibacterium prausnitzii uses an extracellular electron shuttle to grow at oxic-anoxic interphases

M. Tanweer Khan, Sylvia H. Duncan, Alfons J. M. Stams, Jan Maarten van Dijl, Harry J. Flint, Hermie J. M. Harmsen

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Abstract

Faecalibacterium prausnitzii is one of the most abundant bacteria in the human gut ecosystem and it is an important supplier of butyrate to the colonic epithelium. Low numbers of faecalibacteria have been associated with inflammatory bowel disease. Despite being extremely oxygen sensitive, F. prausnitzii is found adherent to the gut mucosa where oxygen diffuses from epithelial cells. This paradox is now explained on the basis of gas tube experiments, flavin-dependent reduction of 5,5'-dithiobis-2-nitrobenzoate and microbial fuel cell experiments. The results show that F. prausnitzii employs an extracellular electron shuttle of flavins and thiols to transfer electrons to oxygen. Both compounds are present in the healthy human gut. Our observations may have important implications for the treatment of patients with Crohn's disease, for example, with flavin-or antioxidant rich diets, and they provide a novel key insight in host-microbe interactions at the gut barrier. The ISME Journal (2012) 6, 1578-1585; doi:10.1038/ismej.2012.5; published online 23 February 2012

Original languageEnglish
Pages (from-to)1578-1585
Number of pages8
JournalThe ISME Journal
Volume6
Issue number8
DOIs
Publication statusPublished - Aug 2012

Cite this

The gut anaerobe Faecalibacterium prausnitzii uses an extracellular electron shuttle to grow at oxic-anoxic interphases. / Khan, M. Tanweer; Duncan, Sylvia H.; Stams, Alfons J. M.; van Dijl, Jan Maarten; Flint, Harry J.; Harmsen, Hermie J. M.

In: The ISME Journal, Vol. 6, No. 8, 08.2012, p. 1578-1585.

Research output: Contribution to journalArticle

Khan, M. Tanweer ; Duncan, Sylvia H. ; Stams, Alfons J. M. ; van Dijl, Jan Maarten ; Flint, Harry J. ; Harmsen, Hermie J. M. / The gut anaerobe Faecalibacterium prausnitzii uses an extracellular electron shuttle to grow at oxic-anoxic interphases. In: The ISME Journal. 2012 ; Vol. 6, No. 8. pp. 1578-1585.
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abstract = "Faecalibacterium prausnitzii is one of the most abundant bacteria in the human gut ecosystem and it is an important supplier of butyrate to the colonic epithelium. Low numbers of faecalibacteria have been associated with inflammatory bowel disease. Despite being extremely oxygen sensitive, F. prausnitzii is found adherent to the gut mucosa where oxygen diffuses from epithelial cells. This paradox is now explained on the basis of gas tube experiments, flavin-dependent reduction of 5,5'-dithiobis-2-nitrobenzoate and microbial fuel cell experiments. The results show that F. prausnitzii employs an extracellular electron shuttle of flavins and thiols to transfer electrons to oxygen. Both compounds are present in the healthy human gut. Our observations may have important implications for the treatment of patients with Crohn's disease, for example, with flavin-or antioxidant rich diets, and they provide a novel key insight in host-microbe interactions at the gut barrier. The ISME Journal (2012) 6, 1578-1585; doi:10.1038/ismej.2012.5; published online 23 February 2012",
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