Abstract
Histone proteins are essential for chromatin formation, and histone gene expression is coupled to DNA synthesis. In metazoans, the histone RNA binding protein HBP/SLBP is involved in post-transcriptional control of histone gene expression. In vitro assays have demonstrated that human HBP/SLBP is involved in histone mRNA 3' end formation and translation. We have inhibited human HBP/SLBP expression by RNA interference to determine its function during the mitotic cell cycle. Inhibition of HBP/SLBP expression resulted in the inhibition of histone gene expression and DNA synthesis, the inhibition of cell cycle progression in S phase and the inhibition of cell proliferation. These findings indicate that human HBP/SLBP is essential for the coordinate synthesis of DNA and histone proteins and is required for progression through the cell division cycle.
Original language | English |
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Pages (from-to) | 6043-6051 |
Number of pages | 9 |
Journal | Journal of Cell Science |
Volume | 117 |
Issue number | 25 |
DOIs | |
Publication status | Published - 1 Dec 2004 |
Keywords
- cell division cycle
- histone gene expression
- DNA synthesis
- loop binding protein
- pre-messenger-RNA
- 3' end formation
- Caenorhabditis elegans
- mammalian-cells
- E/CDK2 substrate
- nuclear RNA
- U7 SNRNP
- in-vivo
- S-phase