The human histone gene expression regulator HBP/SLBP is required for histone and DNA synthesis, cell cycle progression and cell proliferation in mitotic cells

Xiujie Zhao, Susan McKillop-Smith, Berndt Marino Muller

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51 Citations (Scopus)

Abstract

Histone proteins are essential for chromatin formation, and histone gene expression is coupled to DNA synthesis. In metazoans, the histone RNA binding protein HBP/SLBP is involved in post-transcriptional control of histone gene expression. In vitro assays have demonstrated that human HBP/SLBP is involved in histone mRNA 3' end formation and translation. We have inhibited human HBP/SLBP expression by RNA interference to determine its function during the mitotic cell cycle. Inhibition of HBP/SLBP expression resulted in the inhibition of histone gene expression and DNA synthesis, the inhibition of cell cycle progression in S phase and the inhibition of cell proliferation. These findings indicate that human HBP/SLBP is essential for the coordinate synthesis of DNA and histone proteins and is required for progression through the cell division cycle.

Original languageEnglish
Pages (from-to)6043-6051
Number of pages9
JournalJournal of Cell Science
Volume117
Issue number25
DOIs
Publication statusPublished - 1 Dec 2004

Keywords

  • cell division cycle
  • histone gene expression
  • DNA synthesis
  • loop binding protein
  • pre-messenger-RNA
  • 3' end formation
  • Caenorhabditis elegans
  • mammalian-cells
  • E/CDK2 substrate
  • nuclear RNA
  • U7 SNRNP
  • in-vivo
  • S-phase

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