The human Holliday junction resolvase GEN1 rescues the meiotic phenotype of a Schizosaccharomyces pombe mus81 mutant

Alexander Lorenz, Stephen C West, Matthew C Whitby

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Abstract

A key step in meiotic recombination involves the nucleolytic resolution of Holliday junctions to generate crossovers. Although the enzyme that performs this function in human cells is presently unknown, recent studies led to the identification of the XPG-family endonuclease GEN1 that promotes Holliday junction resolution in vitro, suggesting that it may perform a related function in vivo. Here, we show that ectopic expression of GEN1 in fission yeast mus81δ strains results in Holliday junction resolution and crossover formation during meiosis.
Original languageEnglish
Pages (from-to)1866-1873
Number of pages8
JournalNucleic Acids Research
Volume38
Issue number6
Early online date29 Dec 2009
DOIs
Publication statusPublished - Apr 2010

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Holliday Junction Resolvases
Cruciform DNA
Schizosaccharomyces
Phenotype
Endonucleases
Meiosis
Genetic Recombination
Enzymes

Cite this

The human Holliday junction resolvase GEN1 rescues the meiotic phenotype of a Schizosaccharomyces pombe mus81 mutant. / Lorenz, Alexander; West, Stephen C; Whitby, Matthew C.

In: Nucleic Acids Research, Vol. 38, No. 6, 04.2010, p. 1866-1873.

Research output: Contribution to journalArticle

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