The ß-glucan receptor, dectin-1, is predominantly expressed on the surface of cells of the monocytic/macrophage and neutrophil lineages

P. R. Taylor, G. D. Brown, D. M. Reid, J. A. Willment, L. Martinez-Pomares, S. Gordon, Simon Yuk Chun Wong

Research output: Contribution to journalArticle

Abstract

We recently identified dectin-1 (betaGR) as a major beta-glucan receptor on leukocytes and demonstrated that it played a significant role in the non-opsonic recognition of soluble and particulate beta-glucans. Using a novel mAb (2A11) raised against betaGR, we show here that the receptor is not dendritic cell-restricted as first reported, but is broadly expressed, with highest surface expression on populations of myeloid cells (monocyte/macrophage (Mphi) and neutrophil lineages). Dendritic cells and a subpopulation of T cells also expressed the betaGR, but at lower levels. Alveolar Mphi, like inflammatory Mphi, exhibited the highest surface expression of betaGR, indicative of a role for this receptor in immune surveillance. In contrast, resident peritoneal Mphi expressed much lower levels of betaGR on the cell surface. Characterization of the nonopsonic recognition of zymosan by resident peritoneal Mphi suggested the existence of an additional beta-glucan-independent mechanism of zymosan binding that was not observed on elicited or bone marrow-derived Mphi. Although this recognition could be inhibited by mannan, we were able to exclude involvement of the Mphi mannose receptor and complement receptor 3 in this process. These observations imply the existence of an additional mannan-dependent receptor involved in the recognition of zymosan by resident peritoneal Mphi.

Original languageEnglish
Pages (from-to)3876-3882
Number of pages6
JournalThe Journal of Immunology
Volume169
Publication statusPublished - Oct 2002

Keywords

  • MOUSE PERITONEAL-MACROPHAGES
  • BINDING LECTIN SITE
  • MONOCLONAL-ANTIBODY
  • MURINE MACROPHAGES
  • HUMAN-MONOCYTES
  • CR3 CD11B/CD18
  • COMPLEMENT
  • ZYMOSAN
  • PHAGOCYTOSIS
  • IDENTIFICATION

Cite this

Taylor, P. R., Brown, G. D., Reid, D. M., Willment, J. A., Martinez-Pomares, L., Gordon, S., & Wong, S. Y. C. (2002). The ß-glucan receptor, dectin-1, is predominantly expressed on the surface of cells of the monocytic/macrophage and neutrophil lineages. The Journal of Immunology, 169, 3876-3882.

The ß-glucan receptor, dectin-1, is predominantly expressed on the surface of cells of the monocytic/macrophage and neutrophil lineages. / Taylor, P. R.; Brown, G. D.; Reid, D. M.; Willment, J. A.; Martinez-Pomares, L.; Gordon, S.; Wong, Simon Yuk Chun.

In: The Journal of Immunology, Vol. 169, 10.2002, p. 3876-3882.

Research output: Contribution to journalArticle

Taylor, PR, Brown, GD, Reid, DM, Willment, JA, Martinez-Pomares, L, Gordon, S & Wong, SYC 2002, 'The ß-glucan receptor, dectin-1, is predominantly expressed on the surface of cells of the monocytic/macrophage and neutrophil lineages' The Journal of Immunology, vol. 169, pp. 3876-3882.
Taylor, P. R. ; Brown, G. D. ; Reid, D. M. ; Willment, J. A. ; Martinez-Pomares, L. ; Gordon, S. ; Wong, Simon Yuk Chun. / The ß-glucan receptor, dectin-1, is predominantly expressed on the surface of cells of the monocytic/macrophage and neutrophil lineages. In: The Journal of Immunology. 2002 ; Vol. 169. pp. 3876-3882.
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AU - Taylor, P. R.

AU - Brown, G. D.

AU - Reid, D. M.

AU - Willment, J. A.

AU - Martinez-Pomares, L.

AU - Gordon, S.

AU - Wong, Simon Yuk Chun

PY - 2002/10

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N2 - We recently identified dectin-1 (betaGR) as a major beta-glucan receptor on leukocytes and demonstrated that it played a significant role in the non-opsonic recognition of soluble and particulate beta-glucans. Using a novel mAb (2A11) raised against betaGR, we show here that the receptor is not dendritic cell-restricted as first reported, but is broadly expressed, with highest surface expression on populations of myeloid cells (monocyte/macrophage (Mphi) and neutrophil lineages). Dendritic cells and a subpopulation of T cells also expressed the betaGR, but at lower levels. Alveolar Mphi, like inflammatory Mphi, exhibited the highest surface expression of betaGR, indicative of a role for this receptor in immune surveillance. In contrast, resident peritoneal Mphi expressed much lower levels of betaGR on the cell surface. Characterization of the nonopsonic recognition of zymosan by resident peritoneal Mphi suggested the existence of an additional beta-glucan-independent mechanism of zymosan binding that was not observed on elicited or bone marrow-derived Mphi. Although this recognition could be inhibited by mannan, we were able to exclude involvement of the Mphi mannose receptor and complement receptor 3 in this process. These observations imply the existence of an additional mannan-dependent receptor involved in the recognition of zymosan by resident peritoneal Mphi.

AB - We recently identified dectin-1 (betaGR) as a major beta-glucan receptor on leukocytes and demonstrated that it played a significant role in the non-opsonic recognition of soluble and particulate beta-glucans. Using a novel mAb (2A11) raised against betaGR, we show here that the receptor is not dendritic cell-restricted as first reported, but is broadly expressed, with highest surface expression on populations of myeloid cells (monocyte/macrophage (Mphi) and neutrophil lineages). Dendritic cells and a subpopulation of T cells also expressed the betaGR, but at lower levels. Alveolar Mphi, like inflammatory Mphi, exhibited the highest surface expression of betaGR, indicative of a role for this receptor in immune surveillance. In contrast, resident peritoneal Mphi expressed much lower levels of betaGR on the cell surface. Characterization of the nonopsonic recognition of zymosan by resident peritoneal Mphi suggested the existence of an additional beta-glucan-independent mechanism of zymosan binding that was not observed on elicited or bone marrow-derived Mphi. Although this recognition could be inhibited by mannan, we were able to exclude involvement of the Mphi mannose receptor and complement receptor 3 in this process. These observations imply the existence of an additional mannan-dependent receptor involved in the recognition of zymosan by resident peritoneal Mphi.

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KW - MONOCLONAL-ANTIBODY

KW - MURINE MACROPHAGES

KW - HUMAN-MONOCYTES

KW - CR3 CD11B/CD18

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KW - PHAGOCYTOSIS

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JO - The Journal of Immunology

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