The identification of 5'-fluoro-5-deoxyinosine as a shunt product in cell free extracts of Streptomyces cattleya

Steven L. Cobb, Hai Deng, John T. G. Hamilton, Ryan P. McGlinchey, David O'hagan (Corresponding Author), Christoph Schaffrath

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

5′-Fluoro-5′-deoxyinosine (5′-FDI) is identified as an adventitious side product that accumulates in cell free incubations of SAM and fluoride ion in Streptomyces cattleya. 5′-FDI was identified by a combination of isotopic labelling studies and co-synthesis studies as well as enzymatic degradation. Although it is an efficiently generated end product of the cell free incubations, 5′-FDI is not a biosynthetic intermediate and it does not accumulate as a fluorometabolite with fluoroacetate and 4-fluorothreonine in whole cell incubations of S. cattleya. Clearly the purine deaminase which converts 5′-fluoro-5′-deoxyadenosine (5′-FDA) to 5′-FDI in the cell free extract does not come into contact with 5′-FDA in whole cells, suggesting some level of compartmentalisation in cells of S. cattleya. The biotransformation of 5′-FDI from fluoride ion extends the range of organofluorine products, beyond biosynthetic intermediates, that can be generated by this system, for applications such as enzymatic labelling with fluorine-18 for positron emission tomography applications.

Original languageEnglish
Pages (from-to)393-401
Number of pages9
JournalBioorganic Chemistry
Volume33
Issue number5
Early online date13 Sep 2005
DOIs
Publication statusPublished - Oct 2005

Keywords

  • Fluoroacetate
  • 4-Fluorothreonine
  • 5′-Fluoro-5′-deoxyadenosine
  • 5′-Fluoro′-deoxyinosine
  • Fluorometabolite
  • Fluorinase

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