The immune response to corneal allograft requires a site-specific draining lymph node

Jarka Plskova, Linda Duncan, V. Holan, M. Filipec, G. Kraal, John Vincent Forrester

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Background. Recent studies have shown that head-neck draining lymph nodes (DLN) are required for priming the immune response during corneal allograft rejection. In this study we have investigated further the role of the DLN and spleen in corneal graft rejection in mice.

Methods. Individual DLN (submandibular [SM]; superficial cervical [SC]; and internal jugular) or their combinations were removed in mice undergoing corneal allografting (C57BL/10, H2(b) to BALB/c, H2(d)). In some mice, DLN from syngeneic mice were retransplanted, whereas other mice underwent removal of the spleen before corneal allografting. In a high-risk group of mice, removal of the DLN before a second corneal graft. procedure was performed.

Results and Conclusions. The data show that a single specific lymph node, i.e., the SM node, is the major DLN involved in corneal graft rejection whereas its nearest neighbor, the SC DLN, not only cannot substitute for the SM node in priming the immune response but may be involved with the spleen in immune privilege. Retransplantation studies of syngeneic LN indicate that the site of the DLN is more important to the process of graft rejection than the specific DLN tissue. This applies to the DLN whether it contains naive or memory allospecific T cells as shown in experiments in which removal of the SM DLN from mice who had already been primed by a previous corneal graft, prevented rejection of a second corneal graft in the same strain combination.

Original languageEnglish
Pages (from-to)210-215
Number of pages5
JournalTransplantation
Volume73
Issue number2
Publication statusPublished - 2002

Keywords

  • T-CELLS
  • REJECTION
  • PRIVILEGE
  • EYE

Cite this

Plskova, J., Duncan, L., Holan, V., Filipec, M., Kraal, G., & Forrester, J. V. (2002). The immune response to corneal allograft requires a site-specific draining lymph node. Transplantation, 73(2), 210-215.

The immune response to corneal allograft requires a site-specific draining lymph node. / Plskova, Jarka; Duncan, Linda; Holan, V.; Filipec, M.; Kraal, G.; Forrester, John Vincent.

In: Transplantation, Vol. 73, No. 2, 2002, p. 210-215.

Research output: Contribution to journalArticle

Plskova, J, Duncan, L, Holan, V, Filipec, M, Kraal, G & Forrester, JV 2002, 'The immune response to corneal allograft requires a site-specific draining lymph node', Transplantation, vol. 73, no. 2, pp. 210-215.
Plskova J, Duncan L, Holan V, Filipec M, Kraal G, Forrester JV. The immune response to corneal allograft requires a site-specific draining lymph node. Transplantation. 2002;73(2):210-215.
Plskova, Jarka ; Duncan, Linda ; Holan, V. ; Filipec, M. ; Kraal, G. ; Forrester, John Vincent. / The immune response to corneal allograft requires a site-specific draining lymph node. In: Transplantation. 2002 ; Vol. 73, No. 2. pp. 210-215.
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AB - Background. Recent studies have shown that head-neck draining lymph nodes (DLN) are required for priming the immune response during corneal allograft rejection. In this study we have investigated further the role of the DLN and spleen in corneal graft rejection in mice.Methods. Individual DLN (submandibular [SM]; superficial cervical [SC]; and internal jugular) or their combinations were removed in mice undergoing corneal allografting (C57BL/10, H2(b) to BALB/c, H2(d)). In some mice, DLN from syngeneic mice were retransplanted, whereas other mice underwent removal of the spleen before corneal allografting. In a high-risk group of mice, removal of the DLN before a second corneal graft. procedure was performed.Results and Conclusions. The data show that a single specific lymph node, i.e., the SM node, is the major DLN involved in corneal graft rejection whereas its nearest neighbor, the SC DLN, not only cannot substitute for the SM node in priming the immune response but may be involved with the spleen in immune privilege. Retransplantation studies of syngeneic LN indicate that the site of the DLN is more important to the process of graft rejection than the specific DLN tissue. This applies to the DLN whether it contains naive or memory allospecific T cells as shown in experiments in which removal of the SM DLN from mice who had already been primed by a previous corneal graft, prevented rejection of a second corneal graft in the same strain combination.

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