The impact of different DNA extraction kits and laboratories upon the assessment of human gut microbiota composition by 16S rRNA gene sequencing

Nicholas A Kennedy, Alan W Walker, Susan H Berry, Sylvia H Duncan, Freda M Farquarson, Petra Louis, John M Thomson, Jack Satsangi, Harry James Flint, Julian Parkhill, Charlie W Lees, Georgina L Hold, UK IBD Genetics Consortium

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Abstract

Determining bacterial community structure in fecal samples through DNA sequencing is an important facet of intestinal health research. The impact of different commercially available DNA extraction kits upon bacterial community structures has received relatively little attention. The aim of this study was to analyze bacterial communities in volunteer and inflammatory bowel disease (IBD) patient fecal samples extracted using widely used DNA extraction kits in established gastrointestinal research laboratories.
Original languageEnglish
Article numbere88982
JournalPloS ONE
Volume9
Issue number2
DOIs
Publication statusPublished - 24 Feb 2014

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Bacterial Structures
rRNA Genes
intestinal microorganisms
bacterial communities
Genes
ribosomal RNA
DNA
community structure
Chemical analysis
DNA Sequence Analysis
Inflammatory Bowel Diseases
Research
Volunteers
genes
inflammatory bowel disease
Research laboratories
volunteers
Health
sequence analysis
sampling

Cite this

The impact of different DNA extraction kits and laboratories upon the assessment of human gut microbiota composition by 16S rRNA gene sequencing. / Kennedy, Nicholas A; Walker, Alan W; Berry, Susan H; Duncan, Sylvia H; Farquarson, Freda M; Louis, Petra; Thomson, John M; Satsangi, Jack; Flint, Harry James; Parkhill, Julian; Lees, Charlie W; Hold, Georgina L; UK IBD Genetics Consortium.

In: PloS ONE, Vol. 9, No. 2, e88982, 24.02.2014.

Research output: Contribution to journalArticle

Kennedy, NA, Walker, AW, Berry, SH, Duncan, SH, Farquarson, FM, Louis, P, Thomson, JM, Satsangi, J, Flint, HJ, Parkhill, J, Lees, CW, Hold, GL & UK IBD Genetics Consortium 2014, 'The impact of different DNA extraction kits and laboratories upon the assessment of human gut microbiota composition by 16S rRNA gene sequencing' PloS ONE, vol. 9, no. 2, e88982. https://doi.org/10.1371/journal.pone.0088982
Kennedy, Nicholas A ; Walker, Alan W ; Berry, Susan H ; Duncan, Sylvia H ; Farquarson, Freda M ; Louis, Petra ; Thomson, John M ; Satsangi, Jack ; Flint, Harry James ; Parkhill, Julian ; Lees, Charlie W ; Hold, Georgina L ; UK IBD Genetics Consortium. / The impact of different DNA extraction kits and laboratories upon the assessment of human gut microbiota composition by 16S rRNA gene sequencing. In: PloS ONE. 2014 ; Vol. 9, No. 2.
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