The impact of zero-valent iron nanoparticles on a river water bacterial community

Robert J. Barnes, Christopher J. van der Gast, Olga Riba, Laura E. Lehtovirta, James I. Prosser, Peter J. Dobson, Ian P. Thompson

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Zero-valent iron (ZVI) nanoparticles are of interest because of their many potential biomedical and environmental applications. However, these particles have recently been reported to be cytotoxic to bacterial cells. The overall objective of this study was to determine the impact of 100 mg/L ZVI nanoparticles on the diversity and structure of an indigenous river water bacterial community. Response during exposure for 36 days was determined by denaturing gel gradient electrophoresis (DGGE) analysis of bacterial 16S rRNA genes, amplified from extracted DNA, and viable and total cell abundances were determined by plate counting and fluorescent microscopy of DAPI-stained cells. Changes in river water chemistry were also monitored. Addition of ZVI nanoparticles led to a rapid decrease in oxidation-reduction potential (ORP) (+196 to -281 mV) and dissolved oxygen (DO) concentration (8.2-0.6 mg/L), both of which stabilized during the experiment. Interestingly, both viable and total bacterial cell abundances increased and pH decreased, characteristic of an active microbial community. Total community structure was visualized using rank-abundance plots fitted with linear regression models. The slopes of the regression models were used as a descriptive statistic of changes in evenness over time. Importantly, despite bacterial growth, addition of ZVI nanoparticles did not influence bacterial community structure.

Original languageEnglish
Pages (from-to)73-80
Number of pages8
JournalJournal of Hazardous Materials
Volume184
Issue number1-3
Early online date8 Aug 2010
DOIs
Publication statusPublished - 15 Dec 2010

Fingerprint

Rivers
Nanoparticles
river water
Iron
Water
Linear Models
community structure
Bacterial Structures
Denaturing Gradient Gel Electrophoresis
Dissolved oxygen
Electrophoresis
rRNA Genes
Linear regression
water chemistry
Oxidation-Reduction
microscopy
microbial community
Microscopy
dissolved oxygen
electrokinesis

Keywords

  • zero-valent iron nanoparticles
  • river water
  • bacterial community
  • richness
  • recovery
  • escherichia-coli
  • particles
  • trichloroethylene
  • reduction
  • taxa
  • root

Cite this

Barnes, R. J., van der Gast, C. J., Riba, O., Lehtovirta, L. E., Prosser, J. I., Dobson, P. J., & Thompson, I. P. (2010). The impact of zero-valent iron nanoparticles on a river water bacterial community. Journal of Hazardous Materials, 184(1-3), 73-80. https://doi.org/10.1016/j.jhazmat.2010.08.006

The impact of zero-valent iron nanoparticles on a river water bacterial community. / Barnes, Robert J.; van der Gast, Christopher J.; Riba, Olga; Lehtovirta, Laura E.; Prosser, James I.; Dobson, Peter J.; Thompson, Ian P.

In: Journal of Hazardous Materials, Vol. 184, No. 1-3, 15.12.2010, p. 73-80.

Research output: Contribution to journalArticle

Barnes, RJ, van der Gast, CJ, Riba, O, Lehtovirta, LE, Prosser, JI, Dobson, PJ & Thompson, IP 2010, 'The impact of zero-valent iron nanoparticles on a river water bacterial community', Journal of Hazardous Materials, vol. 184, no. 1-3, pp. 73-80. https://doi.org/10.1016/j.jhazmat.2010.08.006
Barnes, Robert J. ; van der Gast, Christopher J. ; Riba, Olga ; Lehtovirta, Laura E. ; Prosser, James I. ; Dobson, Peter J. ; Thompson, Ian P. / The impact of zero-valent iron nanoparticles on a river water bacterial community. In: Journal of Hazardous Materials. 2010 ; Vol. 184, No. 1-3. pp. 73-80.
@article{14b7571db104461d9befa64495c73497,
title = "The impact of zero-valent iron nanoparticles on a river water bacterial community",
abstract = "Zero-valent iron (ZVI) nanoparticles are of interest because of their many potential biomedical and environmental applications. However, these particles have recently been reported to be cytotoxic to bacterial cells. The overall objective of this study was to determine the impact of 100 mg/L ZVI nanoparticles on the diversity and structure of an indigenous river water bacterial community. Response during exposure for 36 days was determined by denaturing gel gradient electrophoresis (DGGE) analysis of bacterial 16S rRNA genes, amplified from extracted DNA, and viable and total cell abundances were determined by plate counting and fluorescent microscopy of DAPI-stained cells. Changes in river water chemistry were also monitored. Addition of ZVI nanoparticles led to a rapid decrease in oxidation-reduction potential (ORP) (+196 to -281 mV) and dissolved oxygen (DO) concentration (8.2-0.6 mg/L), both of which stabilized during the experiment. Interestingly, both viable and total bacterial cell abundances increased and pH decreased, characteristic of an active microbial community. Total community structure was visualized using rank-abundance plots fitted with linear regression models. The slopes of the regression models were used as a descriptive statistic of changes in evenness over time. Importantly, despite bacterial growth, addition of ZVI nanoparticles did not influence bacterial community structure.",
keywords = "zero-valent iron nanoparticles, river water, bacterial community, richness, recovery, escherichia-coli, particles, trichloroethylene, reduction, taxa, root",
author = "Barnes, {Robert J.} and {van der Gast}, {Christopher J.} and Olga Riba and Lehtovirta, {Laura E.} and Prosser, {James I.} and Dobson, {Peter J.} and Thompson, {Ian P.}",
note = "Copyright {\circledC} 2010 Elsevier B.V. All rights reserved.",
year = "2010",
month = "12",
day = "15",
doi = "10.1016/j.jhazmat.2010.08.006",
language = "English",
volume = "184",
pages = "73--80",
journal = "Journal of Hazardous Materials",
issn = "0304-3894",
publisher = "Elsevier",
number = "1-3",

}

TY - JOUR

T1 - The impact of zero-valent iron nanoparticles on a river water bacterial community

AU - Barnes, Robert J.

AU - van der Gast, Christopher J.

AU - Riba, Olga

AU - Lehtovirta, Laura E.

AU - Prosser, James I.

AU - Dobson, Peter J.

AU - Thompson, Ian P.

N1 - Copyright © 2010 Elsevier B.V. All rights reserved.

PY - 2010/12/15

Y1 - 2010/12/15

N2 - Zero-valent iron (ZVI) nanoparticles are of interest because of their many potential biomedical and environmental applications. However, these particles have recently been reported to be cytotoxic to bacterial cells. The overall objective of this study was to determine the impact of 100 mg/L ZVI nanoparticles on the diversity and structure of an indigenous river water bacterial community. Response during exposure for 36 days was determined by denaturing gel gradient electrophoresis (DGGE) analysis of bacterial 16S rRNA genes, amplified from extracted DNA, and viable and total cell abundances were determined by plate counting and fluorescent microscopy of DAPI-stained cells. Changes in river water chemistry were also monitored. Addition of ZVI nanoparticles led to a rapid decrease in oxidation-reduction potential (ORP) (+196 to -281 mV) and dissolved oxygen (DO) concentration (8.2-0.6 mg/L), both of which stabilized during the experiment. Interestingly, both viable and total bacterial cell abundances increased and pH decreased, characteristic of an active microbial community. Total community structure was visualized using rank-abundance plots fitted with linear regression models. The slopes of the regression models were used as a descriptive statistic of changes in evenness over time. Importantly, despite bacterial growth, addition of ZVI nanoparticles did not influence bacterial community structure.

AB - Zero-valent iron (ZVI) nanoparticles are of interest because of their many potential biomedical and environmental applications. However, these particles have recently been reported to be cytotoxic to bacterial cells. The overall objective of this study was to determine the impact of 100 mg/L ZVI nanoparticles on the diversity and structure of an indigenous river water bacterial community. Response during exposure for 36 days was determined by denaturing gel gradient electrophoresis (DGGE) analysis of bacterial 16S rRNA genes, amplified from extracted DNA, and viable and total cell abundances were determined by plate counting and fluorescent microscopy of DAPI-stained cells. Changes in river water chemistry were also monitored. Addition of ZVI nanoparticles led to a rapid decrease in oxidation-reduction potential (ORP) (+196 to -281 mV) and dissolved oxygen (DO) concentration (8.2-0.6 mg/L), both of which stabilized during the experiment. Interestingly, both viable and total bacterial cell abundances increased and pH decreased, characteristic of an active microbial community. Total community structure was visualized using rank-abundance plots fitted with linear regression models. The slopes of the regression models were used as a descriptive statistic of changes in evenness over time. Importantly, despite bacterial growth, addition of ZVI nanoparticles did not influence bacterial community structure.

KW - zero-valent iron nanoparticles

KW - river water

KW - bacterial community

KW - richness

KW - recovery

KW - escherichia-coli

KW - particles

KW - trichloroethylene

KW - reduction

KW - taxa

KW - root

U2 - 10.1016/j.jhazmat.2010.08.006

DO - 10.1016/j.jhazmat.2010.08.006

M3 - Article

C2 - 20810211

VL - 184

SP - 73

EP - 80

JO - Journal of Hazardous Materials

JF - Journal of Hazardous Materials

SN - 0304-3894

IS - 1-3

ER -