The importance of glutathione and phytochelatins on the selenite and arsenate detoxification in Arabidopsis thaliana

Fatai Adigun Aborode, Andrea Raab, Matthias Voigt, Leticia Malta Costa, Eva M Krupp, Joerg Feldmann

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Abstract

We investigated the role of glutathione (GSH) and phytochelatins (PCs) on the detoxification of selenite using Arabidopsis thaliana. The wild-type (WT) of Arabidopsis thaliana and its mutants (glutathione deficient Cad 2-1 and phytochelatins deficient Cad 1-3) were separately exposed to varying concentrations of selenite and arsenate and jointly to both toxicants to determine their sensitivities. The results of the study revealed that, the mutants were about 20-fold more sensitive to arsenate than the WT, an indication that the GSH and PCs affect arsenate detoxification. On the contrary, the WT and both mutants showed a similar level of sensitivity to selenite, an indication that the GSH and PCs do not significantly affect selenite detoxification. However, the WT is about 8 times more sensitive to selenite than to arsenate, and the mutants were more resistant to selenite than arsenate by a factor of 2. This could not be explained by the accumulation of both elements in roots and shoots in exposure experiments. The co-exposure of the WT indicates a synergistic effect with regards to toxicity since selenite did not induce PCs but arsenic and selenium compete in their PC binding as revealed by speciation analysis of the root extracts using HPLC-ICP-MS/ESI-MS. In the absence of PCs an antagonistic effect has been detected which might suggest indirectly that the formation of Se glutathione complex prevent the formation of detrimental selenopeptides. This study, therefore, revealed that PC and GSH have only a subordinate role in the detoxification of selenite.

Original languageEnglish
Pages (from-to)150-161
Number of pages12
JournalJournal of Environmental Sciences
Volume49
Early online date18 Sep 2016
DOIs
Publication statusPublished - Nov 2016

Fingerprint

Phytochelatins
Selenious Acid
Detoxification
selenite
arsenate
detoxification
Glutathione
Selenium
Arsenic
Toxicity
arsenic acid
selenium
arsenic
shoot
Experiments
toxicity
fold

Keywords

  • toxicity
  • phytochelatins
  • glutathione
  • selenopeptides
  • ICP–MS
  • ESI–MS
  • Arabidopsis thaliana

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The importance of glutathione and phytochelatins on the selenite and arsenate detoxification in Arabidopsis thaliana. / Aborode, Fatai Adigun; Raab, Andrea; Voigt, Matthias; Costa, Leticia Malta; Krupp, Eva M; Feldmann, Joerg.

In: Journal of Environmental Sciences, Vol. 49, 11.2016, p. 150-161.

Research output: Contribution to journalArticle

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AU - Aborode, Fatai Adigun

AU - Raab, Andrea

AU - Voigt, Matthias

AU - Costa, Leticia Malta

AU - Krupp, Eva M

AU - Feldmann, Joerg

N1 - This study was supported by the Tesla Research Funds. L.M.C. thanks CNPq (201969/2010-6) for her research visit to Aberdeen and M.V. thanks the European Erasmus Exchange programme.

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N2 - We investigated the role of glutathione (GSH) and phytochelatins (PCs) on the detoxification of selenite using Arabidopsis thaliana. The wild-type (WT) of Arabidopsis thaliana and its mutants (glutathione deficient Cad 2-1 and phytochelatins deficient Cad 1-3) were separately exposed to varying concentrations of selenite and arsenate and jointly to both toxicants to determine their sensitivities. The results of the study revealed that, the mutants were about 20-fold more sensitive to arsenate than the WT, an indication that the GSH and PCs affect arsenate detoxification. On the contrary, the WT and both mutants showed a similar level of sensitivity to selenite, an indication that the GSH and PCs do not significantly affect selenite detoxification. However, the WT is about 8 times more sensitive to selenite than to arsenate, and the mutants were more resistant to selenite than arsenate by a factor of 2. This could not be explained by the accumulation of both elements in roots and shoots in exposure experiments. The co-exposure of the WT indicates a synergistic effect with regards to toxicity since selenite did not induce PCs but arsenic and selenium compete in their PC binding as revealed by speciation analysis of the root extracts using HPLC-ICP-MS/ESI-MS. In the absence of PCs an antagonistic effect has been detected which might suggest indirectly that the formation of Se glutathione complex prevent the formation of detrimental selenopeptides. This study, therefore, revealed that PC and GSH have only a subordinate role in the detoxification of selenite.

AB - We investigated the role of glutathione (GSH) and phytochelatins (PCs) on the detoxification of selenite using Arabidopsis thaliana. The wild-type (WT) of Arabidopsis thaliana and its mutants (glutathione deficient Cad 2-1 and phytochelatins deficient Cad 1-3) were separately exposed to varying concentrations of selenite and arsenate and jointly to both toxicants to determine their sensitivities. The results of the study revealed that, the mutants were about 20-fold more sensitive to arsenate than the WT, an indication that the GSH and PCs affect arsenate detoxification. On the contrary, the WT and both mutants showed a similar level of sensitivity to selenite, an indication that the GSH and PCs do not significantly affect selenite detoxification. However, the WT is about 8 times more sensitive to selenite than to arsenate, and the mutants were more resistant to selenite than arsenate by a factor of 2. This could not be explained by the accumulation of both elements in roots and shoots in exposure experiments. The co-exposure of the WT indicates a synergistic effect with regards to toxicity since selenite did not induce PCs but arsenic and selenium compete in their PC binding as revealed by speciation analysis of the root extracts using HPLC-ICP-MS/ESI-MS. In the absence of PCs an antagonistic effect has been detected which might suggest indirectly that the formation of Se glutathione complex prevent the formation of detrimental selenopeptides. This study, therefore, revealed that PC and GSH have only a subordinate role in the detoxification of selenite.

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KW - ESI–MS

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