The influence of 5'-secondary structures upon ribosome binding to mRNA during translation in yeast

F. A. Sagliocco, M. R.V. Laso, D. Zhu, M. F. Tuite, J. E.G. McCarthy, A. J.P. Brown*

*Corresponding author for this work

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

The influence of 5'-secondary structure formation and 5'-leader length upon mRNA translation in yeast has been analyzed using a closely related set of cat mRNAs (Vega Laso, M. R., Zhu, D., Sagliocco, F. A., Brown, A. J. P., Tuite, M. F., and McCarthy, J. E. G. (1993) J. Biol. Chem. 268, 6453-6462). A cat mRNA with a relatively short unstructured 5'-leader (22 bases) had a ribosome loading about half that of a cat mRNA with an unstructured 5'- leader of 77 bases. The introduction of 5'-secondary structures at various positions throughout the 5'-leader of the cat mRNA inhibited translation initiation, the degree of inhibition being largely dependent upon the thermodynamic stability of the structure. Each mRNA carrying a 5'-secondary structure had a biphasic polysome distribution, indicating that the mRNA molecules were distributed between untranslated and well translated subpopulations. This suggests that once 5'-secondary structures are unwound, they reform slowly relative to the rate of translation initiation in yeast. Untranslated mRNA accumulated in 43 S preinitiation complexes, even when there were only 5 bases between the 5'-cap and the base of the hairpin. The data are consistent with the scanning hypothesis (Kozak, M. (1989) J. Cell. Biol. 108, 229-241) and suggest that 40 S ribosomal subunits bind to mRNA early in the scanning process, probably before mRNA unwinding has taken place.

Original languageEnglish
Pages (from-to)26522-26530
Number of pages9
JournalJournal of Biological Chemistry
Volume268
Issue number35
Publication statusPublished - 1 Jan 1993

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Protein Biosynthesis
Ribosomes
Yeast
Yeasts
Messenger RNA
Cats
Ribosome Subunits
Polyribosomes
Scanning
Thermodynamics
Thermodynamic stability
Molecules

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Sagliocco, F. A., Laso, M. R. V., Zhu, D., Tuite, M. F., McCarthy, J. E. G., & Brown, A. J. P. (1993). The influence of 5'-secondary structures upon ribosome binding to mRNA during translation in yeast. Journal of Biological Chemistry, 268(35), 26522-26530.

The influence of 5'-secondary structures upon ribosome binding to mRNA during translation in yeast. / Sagliocco, F. A.; Laso, M. R.V.; Zhu, D.; Tuite, M. F.; McCarthy, J. E.G.; Brown, A. J.P.

In: Journal of Biological Chemistry, Vol. 268, No. 35, 01.01.1993, p. 26522-26530.

Research output: Contribution to journalArticle

Sagliocco, FA, Laso, MRV, Zhu, D, Tuite, MF, McCarthy, JEG & Brown, AJP 1993, 'The influence of 5'-secondary structures upon ribosome binding to mRNA during translation in yeast', Journal of Biological Chemistry, vol. 268, no. 35, pp. 26522-26530.
Sagliocco FA, Laso MRV, Zhu D, Tuite MF, McCarthy JEG, Brown AJP. The influence of 5'-secondary structures upon ribosome binding to mRNA during translation in yeast. Journal of Biological Chemistry. 1993 Jan 1;268(35):26522-26530.
Sagliocco, F. A. ; Laso, M. R.V. ; Zhu, D. ; Tuite, M. F. ; McCarthy, J. E.G. ; Brown, A. J.P. / The influence of 5'-secondary structures upon ribosome binding to mRNA during translation in yeast. In: Journal of Biological Chemistry. 1993 ; Vol. 268, No. 35. pp. 26522-26530.
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