TY - JOUR
T1 - The influence of 5'-secondary structures upon ribosome binding to mRNA during translation in yeast
AU - Sagliocco, F. A.
AU - Laso, M. R.V.
AU - Zhu, D.
AU - Tuite, M. F.
AU - McCarthy, J. E.G.
AU - Brown, A. J.P.
PY - 1993/1/1
Y1 - 1993/1/1
N2 - The influence of 5'-secondary structure formation and 5'-leader length upon mRNA translation in yeast has been analyzed using a closely related set of cat mRNAs (Vega Laso, M. R., Zhu, D., Sagliocco, F. A., Brown, A. J. P., Tuite, M. F., and McCarthy, J. E. G. (1993) J. Biol. Chem. 268, 6453-6462). A cat mRNA with a relatively short unstructured 5'-leader (22 bases) had a ribosome loading about half that of a cat mRNA with an unstructured 5'- leader of 77 bases. The introduction of 5'-secondary structures at various positions throughout the 5'-leader of the cat mRNA inhibited translation initiation, the degree of inhibition being largely dependent upon the thermodynamic stability of the structure. Each mRNA carrying a 5'-secondary structure had a biphasic polysome distribution, indicating that the mRNA molecules were distributed between untranslated and well translated subpopulations. This suggests that once 5'-secondary structures are unwound, they reform slowly relative to the rate of translation initiation in yeast. Untranslated mRNA accumulated in 43 S preinitiation complexes, even when there were only 5 bases between the 5'-cap and the base of the hairpin. The data are consistent with the scanning hypothesis (Kozak, M. (1989) J. Cell. Biol. 108, 229-241) and suggest that 40 S ribosomal subunits bind to mRNA early in the scanning process, probably before mRNA unwinding has taken place.
AB - The influence of 5'-secondary structure formation and 5'-leader length upon mRNA translation in yeast has been analyzed using a closely related set of cat mRNAs (Vega Laso, M. R., Zhu, D., Sagliocco, F. A., Brown, A. J. P., Tuite, M. F., and McCarthy, J. E. G. (1993) J. Biol. Chem. 268, 6453-6462). A cat mRNA with a relatively short unstructured 5'-leader (22 bases) had a ribosome loading about half that of a cat mRNA with an unstructured 5'- leader of 77 bases. The introduction of 5'-secondary structures at various positions throughout the 5'-leader of the cat mRNA inhibited translation initiation, the degree of inhibition being largely dependent upon the thermodynamic stability of the structure. Each mRNA carrying a 5'-secondary structure had a biphasic polysome distribution, indicating that the mRNA molecules were distributed between untranslated and well translated subpopulations. This suggests that once 5'-secondary structures are unwound, they reform slowly relative to the rate of translation initiation in yeast. Untranslated mRNA accumulated in 43 S preinitiation complexes, even when there were only 5 bases between the 5'-cap and the base of the hairpin. The data are consistent with the scanning hypothesis (Kozak, M. (1989) J. Cell. Biol. 108, 229-241) and suggest that 40 S ribosomal subunits bind to mRNA early in the scanning process, probably before mRNA unwinding has taken place.
UR - http://www.scopus.com/inward/record.url?scp=0027380401&partnerID=8YFLogxK
M3 - Article
C2 - 8253781
AN - SCOPUS:0027380401
SN - 0021-9258
VL - 268
SP - 26522
EP - 26530
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 35
ER -