The influence of culture medium composition on drug metabolising enzyme activities of the human liver derived Hep G2 cell line

H Doostdar, S J Duthie, M D Burke, W T Melvin, M H Grant

    Research output: Contribution to journalArticle

    69 Citations (Scopus)

    Abstract

    When grown in the standard Dulbecco's medium the human liver derived Hep G2 hepatoma cell line shows only 10-20% of the cytochrome P-450-dependent mixed function oxidase (MFO) activity of freshly isolated human adult hepatocytes. However, the MFO activities and, to a lesser extent, the activities of UDP-glucuronyltransferase and glutathione-S-transferase can be increased by altering the composition of the growth medium. Modified Earle's medium was more effective in this respect than Williams' E medium and increased the O-dealkylations of ethoxyresorufin, benzyloxyresorufin and pentoxyresorufin 50-, 30- and 10-fold, respectively.
    Original languageEnglish
    Pages (from-to)15-8
    Number of pages4
    JournalFEBS Letters
    Volume241
    Issue number1-2
    Publication statusPublished - 1988

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    Hep G2 Cells
    Enzyme activity
    Mixed Function Oxygenases
    Human Activities
    Liver
    Culture Media
    Cells
    Dealkylation
    Glucuronosyltransferase
    Uridine Diphosphate
    Enzymes
    Glutathione Transferase
    Chemical analysis
    Pharmaceutical Preparations
    Cytochrome P-450 Enzyme System
    Hepatocytes
    Hepatocellular Carcinoma
    Growth
    William's medium E
    pentoxyresorufin

    Keywords

    • Carcinoma, Hepatocellular
    • Cell Line
    • Culture Media
    • Cytochrome P-450 Enzyme System
    • Glucuronosyltransferase
    • Glutathione
    • Glutathione Transferase
    • Humans
    • Kinetics
    • Liver Neoplasms
    • Mixed Function Oxygenases

    Cite this

    Doostdar, H., Duthie, S. J., Burke, M. D., Melvin, W. T., & Grant, M. H. (1988). The influence of culture medium composition on drug metabolising enzyme activities of the human liver derived Hep G2 cell line. FEBS Letters, 241(1-2), 15-8.

    The influence of culture medium composition on drug metabolising enzyme activities of the human liver derived Hep G2 cell line. / Doostdar, H; Duthie, S J; Burke, M D; Melvin, W T; Grant, M H.

    In: FEBS Letters, Vol. 241, No. 1-2, 1988, p. 15-8.

    Research output: Contribution to journalArticle

    Doostdar, H, Duthie, SJ, Burke, MD, Melvin, WT & Grant, MH 1988, 'The influence of culture medium composition on drug metabolising enzyme activities of the human liver derived Hep G2 cell line', FEBS Letters, vol. 241, no. 1-2, pp. 15-8.
    Doostdar, H ; Duthie, S J ; Burke, M D ; Melvin, W T ; Grant, M H. / The influence of culture medium composition on drug metabolising enzyme activities of the human liver derived Hep G2 cell line. In: FEBS Letters. 1988 ; Vol. 241, No. 1-2. pp. 15-8.
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    AB - When grown in the standard Dulbecco's medium the human liver derived Hep G2 hepatoma cell line shows only 10-20% of the cytochrome P-450-dependent mixed function oxidase (MFO) activity of freshly isolated human adult hepatocytes. However, the MFO activities and, to a lesser extent, the activities of UDP-glucuronyltransferase and glutathione-S-transferase can be increased by altering the composition of the growth medium. Modified Earle's medium was more effective in this respect than Williams' E medium and increased the O-dealkylations of ethoxyresorufin, benzyloxyresorufin and pentoxyresorufin 50-, 30- and 10-fold, respectively.

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