Abstract
When grown in the standard Dulbecco's medium the human liver derived Hep G2 hepatoma cell line shows only 10-20% of the cytochrome P-450-dependent mixed function oxidase (MFO) activity of freshly isolated human adult hepatocytes. However, the MFO activities and, to a lesser extent, the activities of UDP-glucuronyltransferase and glutathione-S-transferase can be increased by altering the composition of the growth medium. Modified Earle's medium was more effective in this respect than Williams' E medium and increased the O-dealkylations of ethoxyresorufin, benzyloxyresorufin and pentoxyresorufin 50-, 30- and 10-fold, respectively.
| Original language | English |
|---|---|
| Pages (from-to) | 15-8 |
| Number of pages | 4 |
| Journal | FEBS Letters |
| Volume | 241 |
| Issue number | 1-2 |
| Publication status | Published - 1988 |
Keywords
- Carcinoma, Hepatocellular
- Cell Line
- Culture Media
- Cytochrome P-450 Enzyme System
- Glucuronosyltransferase
- Glutathione
- Glutathione Transferase
- Humans
- Kinetics
- Liver Neoplasms
- Mixed Function Oxygenases