When grown in the standard Dulbecco's medium the human liver derived Hep G2 hepatoma cell line shows only 10-20% of the cytochrome P-450-dependent mixed function oxidase (MFO) activity of freshly isolated human adult hepatocytes. However, the MFO activities and, to a lesser extent, the activities of UDP-glucuronyltransferase and glutathione-S-transferase can be increased by altering the composition of the growth medium. Modified Earle's medium was more effective in this respect than Williams' E medium and increased the O-dealkylations of ethoxyresorufin, benzyloxyresorufin and pentoxyresorufin 50-, 30- and 10-fold, respectively.
|Number of pages||4|
|Publication status||Published - 1988|
- Carcinoma, Hepatocellular
- Cell Line
- Culture Media
- Cytochrome P-450 Enzyme System
- Glutathione Transferase
- Liver Neoplasms
- Mixed Function Oxygenases
Doostdar, H., Duthie, S. J., Burke, M. D., Melvin, W. T., & Grant, M. H. (1988). The influence of culture medium composition on drug metabolising enzyme activities of the human liver derived Hep G2 cell line. FEBS Letters, 241(1-2), 15-8.