The Influence of Smoking and Diet on the Hypoxanthine Phosphoribosyltransferase (HPRT) Mutant Frequency in Circulating T-Lymphocytes from a Normal Human-Population

Susan Joyce Duthie, M Ross, A R Collins

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    28 Citations (Scopus)

    Abstract

    The influence of the dietary antioxidants vitamin C, alpha- and beta-carotene, lycopene, lutein/zeaxanthin, phytofluene, P-cryptoxanthin, retinol and alpha- and gamma-tocopherol on the hypoxanthine phosphoribosyltransferase (hprt) mutant frequency in human peripheral T lymphocytes was investigated. Twenty-five male non-smokers and 27 male smokers in the age range 50-59 years were recruited. Smokers showed a significantly higher mutant frequency compared with non-smokers (x 1.5, P < 0.01). In addition, there was a significant positive relationship between hprt mutant frequency and the number of cigarettes that individuals reported smoking daily (P < 0.01). Smokers showed significantly lower levels of plasma vitamin C and the carotenoid alpha-carotene than non-smokers (P < 0.01 and P < 0.05 respectively). Both hprt mutant frequency and lymphocyte plating efficiency were weakly inversely associated with plasma vitamin C levels (P < 0.07 and P < 0.06 respectively) suggesting that vitamin C may be protective against mutation at the hprt locus. This relationship was markedly stronger in smokers (P < 0.01).

    Original languageEnglish
    Pages (from-to)55-64
    Number of pages10
    JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
    Volume331
    Issue number1
    DOIs
    Publication statusPublished - Sep 1995

    Keywords

    • HPRT MUTANT
    • HUMAN LYMPHOCYTES
    • ANTIOXIDANT
    • DIET
    • BIOMARKER
    • FACTORS INFLUENCING MUTATION
    • CIGARETTE-SMOKE
    • CANCER PREVENTION
    • EPIDEMIOLOGIC EVIDENCE
    • BREAST-CANCER
    • CLONING ASSAY
    • FREE-RADICALS
    • HUMAN-DISEASE
    • DNA DAMAGE
    • VITAMIN-C
    • HPRT mutant
    • human lymphocytes
    • antioxidant
    • diet
    • biomarker

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