Abstract
The influence of the dietary antioxidants vitamin C, alpha- and beta-carotene, lycopene, lutein/zeaxanthin, phytofluene, P-cryptoxanthin, retinol and alpha- and gamma-tocopherol on the hypoxanthine phosphoribosyltransferase (hprt) mutant frequency in human peripheral T lymphocytes was investigated. Twenty-five male non-smokers and 27 male smokers in the age range 50-59 years were recruited. Smokers showed a significantly higher mutant frequency compared with non-smokers (x 1.5, P < 0.01). In addition, there was a significant positive relationship between hprt mutant frequency and the number of cigarettes that individuals reported smoking daily (P < 0.01). Smokers showed significantly lower levels of plasma vitamin C and the carotenoid alpha-carotene than non-smokers (P < 0.01 and P < 0.05 respectively). Both hprt mutant frequency and lymphocyte plating efficiency were weakly inversely associated with plasma vitamin C levels (P < 0.07 and P < 0.06 respectively) suggesting that vitamin C may be protective against mutation at the hprt locus. This relationship was markedly stronger in smokers (P < 0.01).
Original language | English |
---|---|
Pages (from-to) | 55-64 |
Number of pages | 10 |
Journal | Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis |
Volume | 331 |
Issue number | 1 |
DOIs | |
Publication status | Published - Sep 1995 |
Keywords
- HPRT MUTANT
- HUMAN LYMPHOCYTES
- ANTIOXIDANT
- DIET
- BIOMARKER
- FACTORS INFLUENCING MUTATION
- CIGARETTE-SMOKE
- CANCER PREVENTION
- EPIDEMIOLOGIC EVIDENCE
- BREAST-CANCER
- CLONING ASSAY
- FREE-RADICALS
- HUMAN-DISEASE
- DNA DAMAGE
- VITAMIN-C
- HPRT mutant
- human lymphocytes
- antioxidant
- diet
- biomarker