The major 36 kDa Neospora caninum tachyzoite surface protein is closely related to the major Toxoplasma gondii surface antigen

S Sonda, N Fuchs, Bernadette Connolly, P Fernandez, B Gottstein, A Hemphill (Corresponding Author)

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The tachyzoites and the tissue cysts containing bradyzoites of Neospora caninum and Toxoplasma gondii, respectively, are difficult to distinguish morphologically. Specific antigens have been identified in T. gondii tachyzoites and bradyzoites, some of which are stage-specifically expressed, and different functions have been attributed to some of them. A tachyzoite stage-specifically expressed surface protein is the major surface antigen 1 (SAG1) which has been shown to be involved in host cell attachment and invasion. Previously we have identified a cell surface-associated glycoprotein (p36) in N. caninum tachyzoites. The full length coding sequence of the cDNA coding for p36 was determined, and analysis of the deduced amino acid sequence demonstrated that p36 is closely related to SAG1. p36 is encoded by a single copy gene which produces a transcript of 1.4 kb. Immunogold labeling of resin-embedded parasites using polyclonal antibodies affinity-purified on a recombinant p36 fusion protein expressed in Escherichia coli showed that this protein is located exclusively on the tachyzoite cell surface. As SAG1 in T. gondii, p36 is expressed in the tachyzoite stage, but is absent from bradyzoites. p36 is recognized by antibodies present in sera of cows experimentally infected with N. caninum tachyzoites.
Original languageEnglish
Pages (from-to)97-108
Number of pages12
JournalMolecular and Biochemical Parasitology
Volume97
Issue number1-2
DOIs
Publication statusPublished - 30 Nov 1998

Fingerprint

Neospora
Toxoplasma
Surface Antigens
Recombinant Fusion Proteins
Antibody Affinity
Escherichia coli Proteins
Membrane Glycoproteins
Protein Sequence Analysis
Cysts
Membrane Proteins
Parasites
Complementary DNA
Antigens
Antibodies
Serum
Genes
tachyzoite surface antigen p36 protein, Neospora caninum

Keywords

  • Amino Acid Sequence
  • Animals
  • Antigens, Protozoan
  • Antigens, Surface
  • Blotting, Western
  • Brain
  • Cattle
  • Cercopithecus aethiops
  • DNA, Complementary
  • Fluorescent Antibody Technique, Direct
  • Immunohistochemistry
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred ICR
  • Microscopy, Electron
  • Molecular Sequence Data
  • Neospora
  • Protozoan Proteins
  • Recombinant Proteins
  • Sequence Alignment
  • Toxoplasma
  • Vero Cells

Cite this

The major 36 kDa Neospora caninum tachyzoite surface protein is closely related to the major Toxoplasma gondii surface antigen. / Sonda, S; Fuchs, N; Connolly, Bernadette; Fernandez, P; Gottstein, B; Hemphill, A (Corresponding Author).

In: Molecular and Biochemical Parasitology, Vol. 97, No. 1-2, 30.11.1998, p. 97-108.

Research output: Contribution to journalArticle

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abstract = "The tachyzoites and the tissue cysts containing bradyzoites of Neospora caninum and Toxoplasma gondii, respectively, are difficult to distinguish morphologically. Specific antigens have been identified in T. gondii tachyzoites and bradyzoites, some of which are stage-specifically expressed, and different functions have been attributed to some of them. A tachyzoite stage-specifically expressed surface protein is the major surface antigen 1 (SAG1) which has been shown to be involved in host cell attachment and invasion. Previously we have identified a cell surface-associated glycoprotein (p36) in N. caninum tachyzoites. The full length coding sequence of the cDNA coding for p36 was determined, and analysis of the deduced amino acid sequence demonstrated that p36 is closely related to SAG1. p36 is encoded by a single copy gene which produces a transcript of 1.4 kb. Immunogold labeling of resin-embedded parasites using polyclonal antibodies affinity-purified on a recombinant p36 fusion protein expressed in Escherichia coli showed that this protein is located exclusively on the tachyzoite cell surface. As SAG1 in T. gondii, p36 is expressed in the tachyzoite stage, but is absent from bradyzoites. p36 is recognized by antibodies present in sera of cows experimentally infected with N. caninum tachyzoites.",
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T1 - The major 36 kDa Neospora caninum tachyzoite surface protein is closely related to the major Toxoplasma gondii surface antigen

AU - Sonda, S

AU - Fuchs, N

AU - Connolly, Bernadette

AU - Fernandez, P

AU - Gottstein, B

AU - Hemphill, A

PY - 1998/11/30

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N2 - The tachyzoites and the tissue cysts containing bradyzoites of Neospora caninum and Toxoplasma gondii, respectively, are difficult to distinguish morphologically. Specific antigens have been identified in T. gondii tachyzoites and bradyzoites, some of which are stage-specifically expressed, and different functions have been attributed to some of them. A tachyzoite stage-specifically expressed surface protein is the major surface antigen 1 (SAG1) which has been shown to be involved in host cell attachment and invasion. Previously we have identified a cell surface-associated glycoprotein (p36) in N. caninum tachyzoites. The full length coding sequence of the cDNA coding for p36 was determined, and analysis of the deduced amino acid sequence demonstrated that p36 is closely related to SAG1. p36 is encoded by a single copy gene which produces a transcript of 1.4 kb. Immunogold labeling of resin-embedded parasites using polyclonal antibodies affinity-purified on a recombinant p36 fusion protein expressed in Escherichia coli showed that this protein is located exclusively on the tachyzoite cell surface. As SAG1 in T. gondii, p36 is expressed in the tachyzoite stage, but is absent from bradyzoites. p36 is recognized by antibodies present in sera of cows experimentally infected with N. caninum tachyzoites.

AB - The tachyzoites and the tissue cysts containing bradyzoites of Neospora caninum and Toxoplasma gondii, respectively, are difficult to distinguish morphologically. Specific antigens have been identified in T. gondii tachyzoites and bradyzoites, some of which are stage-specifically expressed, and different functions have been attributed to some of them. A tachyzoite stage-specifically expressed surface protein is the major surface antigen 1 (SAG1) which has been shown to be involved in host cell attachment and invasion. Previously we have identified a cell surface-associated glycoprotein (p36) in N. caninum tachyzoites. The full length coding sequence of the cDNA coding for p36 was determined, and analysis of the deduced amino acid sequence demonstrated that p36 is closely related to SAG1. p36 is encoded by a single copy gene which produces a transcript of 1.4 kb. Immunogold labeling of resin-embedded parasites using polyclonal antibodies affinity-purified on a recombinant p36 fusion protein expressed in Escherichia coli showed that this protein is located exclusively on the tachyzoite cell surface. As SAG1 in T. gondii, p36 is expressed in the tachyzoite stage, but is absent from bradyzoites. p36 is recognized by antibodies present in sera of cows experimentally infected with N. caninum tachyzoites.

KW - Amino Acid Sequence

KW - Animals

KW - Antigens, Protozoan

KW - Antigens, Surface

KW - Blotting, Western

KW - Brain

KW - Cattle

KW - Cercopithecus aethiops

KW - DNA, Complementary

KW - Fluorescent Antibody Technique, Direct

KW - Immunohistochemistry

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KW - Mice

KW - Mice, Inbred BALB C

KW - Mice, Inbred ICR

KW - Microscopy, Electron

KW - Molecular Sequence Data

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KW - Protozoan Proteins

KW - Recombinant Proteins

KW - Sequence Alignment

KW - Toxoplasma

KW - Vero Cells

U2 - 10.1016/S0166-6851(98)00133-9

DO - 10.1016/S0166-6851(98)00133-9

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JO - Molecular and Biochemical Parasitology

JF - Molecular and Biochemical Parasitology

SN - 0166-6851

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