The manufacture and assessment of tissue microarrays: suggestions and criteria for analysis, with breast cancer as an example

Sarah E. Pinder, John P. Brown, Cheryl Gillett, Colin A. Purdie, Valerie Speirs, Alastair M. Thompson, Abeer M. Shaaban, Translational Subgroup of the NCRI Breast Clinical Studies Group

Research output: Contribution to journalReview article

25 Citations (Scopus)

Abstract

Tissue microarray (TMA) is an established and valuable tool, particularly in translational research and clinical trials, allowing resource-efficient use, and high-throughput profiling, of large numbers of tumours. Despite this, there is little evidence, or guidance, on the optimum manufacture, use and assessment of TMAs. Here we review some of the literature, using breast cancer as an example, to highlight good practice and pitfalls in the design and manufacture of TMAs. Issues, such as the size, number, spacing and layout of cores, as well as the assessment and reporting of studies using TMAs are addressed. We make some suggestions regarding these challenges, and propose a checklist of features that should be considered in order to stimulate debate and improve the quality of data produced by TMA analysis.

Original languageEnglish
Pages (from-to)169-177
Number of pages9
JournalJournal of Clinical Pathology
Volume66
Issue number3
Early online date19 Oct 2012
DOIs
Publication statusPublished - Mar 2013

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    Pinder, S. E., Brown, J. P., Gillett, C., Purdie, C. A., Speirs, V., Thompson, A. M., Shaaban, A. M., & Translational Subgroup of the NCRI Breast Clinical Studies Group (2013). The manufacture and assessment of tissue microarrays: suggestions and criteria for analysis, with breast cancer as an example. Journal of Clinical Pathology, 66(3), 169-177. https://doi.org/10.1136/jclinpath-2012-201091