The myeloid lineage is required for the emergence of a regeneration-permissive environment following Xenopus tail amputation

Can Aztekin, Tom W. Hiscock, Richard Butler, Francisco De Jesús Andino, Jacques Robert, John B. Gurdon, Jerome Jullien*

*Corresponding author for this work

Research output: Contribution to journalArticle

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Abstract

Regeneration-competent vertebrates are considered to suppress inflammation faster than non-regenerating ones. Hence, understanding the cellularmechanisms affected by immune cells and inflammation can help develop strategies to promote tissue repair and regeneration. Here, we took advantage of naturally occurring tail regeneration-competent and -incompetent developmental stages of Xenopus tadpoles. We first establish the essential role of the myeloid lineage for tail regeneration in the regeneration-competent tadpoles. We then reveal that upon tail amputation there is a myeloid lineage-dependent change in amputationinduced apoptosis levels, which in turn promotes tissue remodelling, and ultimately leads to the relocalization of the regeneration-organizing cells responsible for progenitor proliferation. These cellularmechanisms failed to be executed in regeneration-incompetent tadpoles. We demonstrate that regeneration incompetency is characterized by inflammatory myeloid cells whereas regeneration competency is associated with reparative myeloid cells. Moreover, treatment of regeneration-incompetent tadpoles with immune-suppressing drugs restores myeloid lineage-controlled cellular mechanisms. Collectively, our work reveals the effects of differential activation of the myeloid lineage on the creation of a regeneration-permissive environment and could be further exploited to devise strategies for regenerative medicine purposes.

Original languageEnglish
Article number185496
JournalDevelopment (Cambridge)
Volume147
Issue number3
Early online date27 Jan 2020
DOIs
Publication statusPublished - 5 Feb 2020

Keywords

  • Inflammation
  • Macrophages
  • Myeloid lineage
  • Neutrophils
  • Tail regeneration
  • Xenopus
  • MACROPHAGES
  • MODEL
  • IDENTIFICATION
  • HYALURONIC-ACID
  • LIMB
  • TRANSGENESIS
  • AMPHIBIANS
  • INFLAMMATION
  • CELL

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

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