The Nature of Arsenic-Phytochelatin Complexes in Holcus lanatus and Pteris cretica

Andrea Raab, Jorg Feldmann, Andrew Alexander Meharg

Research output: Contribution to journalArticle

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Abstract

We have developed a method to extract and separate phytochelatins (PCs)-metal(loid) complexes using parallel metal(loid)-specific (inductively coupled plasma-mass spectrometry) and organic-specific (electrospray ionization-mass spectrometry) detection systems-and use it here to ascertain the nature of arsenic (As)-PC complexes in plant extracts. This study is the first unequivocal report, to our knowledge, of PC complex coordination chemistry in plant extracts for any metal or metalloid ion. The As-tolerant grass Holcus lanatus and the As hyperaccumulator Pteris cretica were used as model plants. In an in vitro experiment using a mixture of reduced glutathione (GS), PC2, and PC3, As preferred the formation of the arsenite [As-(III)]-PC3 complex over GS-As-(III)-PC2, As-(III)-(GS)(3), As-(III)-PC2, or As-(III)-(PC2)(2) (GS: glutathione bound to arsenic via sulphur of cysteine). In H. lanatus, the As-(III)-PC3 complex was the dominant complex, although reduced glutathione, PC2, and PC3 were found in the extract. P. cretica only synthesizes PC2 and forms dominantly the GS-As-(III)-PC2 complex. This is the first evidence, to our knowledge, for the existence of mixed glutathione-PC-metal(loid) complexes in plant tissues or in vitro. In both plant species, As is dominantly in non-bound inorganic forms, with 13% being present in PC complexes for H. lanatus and 1% in P. cretica.

Original languageEnglish
Pages (from-to)1113-1122
Number of pages9
JournalPlant Physiology
Volume134
DOIs
Publication statusPublished - Mar 2004

Fingerprint

Holcus
Pteris
Pteris cretica
Phytochelatins
Holcus lanatus
phytochelatins
Arsenic
arsenic
Glutathione
glutathione
Coordination Complexes
metals
Plant Extracts
plant extracts
coordination compounds
Metals
Metalloids
arsenites
hyperaccumulators
Electrospray Ionization Mass Spectrometry

Keywords

  • Heavy-metal detoxification
  • Cadmium-phytochelatin
  • Binding-properties
  • Uptake kinetics
  • Tolerance
  • Vittata
  • Plants
  • Hyperaccumulation
  • Accumulation
  • Glutathione

Cite this

The Nature of Arsenic-Phytochelatin Complexes in Holcus lanatus and Pteris cretica. / Raab, Andrea; Feldmann, Jorg; Meharg, Andrew Alexander.

In: Plant Physiology, Vol. 134, 03.2004, p. 1113-1122.

Research output: Contribution to journalArticle

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