TY - JOUR
T1 - The phosphocholine and glycerophosphocholine content of an oestrogen- sensitive rat mammary tumour correlates strongly with growth rate
AU - Smith, Timothy Andrew Davies
AU - Eccles, S A
AU - Ormerod, M
AU - Tombs, A
AU - Titley, Jenny
AU - McReady, Ralph
PY - 1991
Y1 - 1991
N2 - An oestrogen sensitive rat mammary tumour was grown in two groups of female and one group of male hooded rats. The male group and one of the female groups were supplemented with oestrogen. The tumours grew most rapidly in the female supplemented group. When the tumours reached 1.5 cm in diameter they were harvested and the cell cycle distribution and number of cells actively synthesising DNA (bromodeox- yuridine (BrdU) labelling index) determined in each case. Chemical extracts were prepared from each tumour and the concentration of phosphorus-containing metabolites determined using high resolution NMR spectroscopy. The concentration of phosphocholine was found to correlate strongly with the number of cells in S-phase and the number of cells labelled with BrdU, whilst a highly significant negative correlation was observed between these two parameters and glycerophosphocholine. The concentration of phos- phoethanolamine did not correlate with either of these measures of proliferation rate. The concentration of glycerophosphorylethanolamine showed a weak negative correlation with the number of cells in S-phase. © Macmillan Press Ltd., 1991.
AB - An oestrogen sensitive rat mammary tumour was grown in two groups of female and one group of male hooded rats. The male group and one of the female groups were supplemented with oestrogen. The tumours grew most rapidly in the female supplemented group. When the tumours reached 1.5 cm in diameter they were harvested and the cell cycle distribution and number of cells actively synthesising DNA (bromodeox- yuridine (BrdU) labelling index) determined in each case. Chemical extracts were prepared from each tumour and the concentration of phosphorus-containing metabolites determined using high resolution NMR spectroscopy. The concentration of phosphocholine was found to correlate strongly with the number of cells in S-phase and the number of cells labelled with BrdU, whilst a highly significant negative correlation was observed between these two parameters and glycerophosphocholine. The concentration of phos- phoethanolamine did not correlate with either of these measures of proliferation rate. The concentration of glycerophosphorylethanolamine showed a weak negative correlation with the number of cells in S-phase. © Macmillan Press Ltd., 1991.
UR - http://www.scopus.com/inward/record.url?scp=0025953742&partnerID=8YFLogxK
U2 - 10.1038/bjc.1991.407
DO - 10.1038/bjc.1991.407
M3 - Article
C2 - 1931601
VL - 64
SP - 821
EP - 826
JO - British Journal of Cancer
JF - British Journal of Cancer
SN - 0007-0920
IS - 5
ER -