The porcine acute phase protein response to acute clinical and subclinical experimental infection with Streptococcus suis

N. S. Sorensen, C. Tegtmeier, L. O. Andresen, M. Pineiro, M. J. M. Toussaint, Fiona Margaret Campbell, F. Lampreave, P. M. H. Heegaard

    Research output: Contribution to journalArticle

    77 Citations (Scopus)

    Abstract

    The pig acute phase protein (APP) response to experimental Streptococcus suis (S. suis) infection was mapped by the measurement of the positive APPs C-reactive protein (CRP), serum amyloid A (SAA), haptoglobin (Hp) and major acute phase protein (pig-MAP) and the negative APPs albumin and apolipoprotein (Apo) A-I. The aim was to elucidate the differences in the acute phase behaviour of the individual APPs during a typical bacterial septicaemic, infection. Pigs were inoculated subcutaneously with live S. suis serotype 2 and blood was sampled before and on various days post inoculation (p.i.), until the pigs were killed and autopsied on day 14 p.i. Clinical signs (fever and lameness) were observed in four of the five inoculated pigs from day 2 p.i., and these pigs also had arthritic lesions at autopsy. CRP and SAA showed fast increases in serum concentrations, CRP being elevated from days 1 to 12 p.i. and peaking at 10 times the day 0-levels on day 1 p.i. SAA rose quickly to peak levels of 30-40 times the day 0-level on days 1-2 and returned to pre-inoculation level on day 5 p.i. Hp and pig-MAP showed slightly slower responses, both peaking around 5 days p.i. Hp was increased throughout the experiment with maximum levels around 10 times the day 0-levels, and pig-MAP was elevated on days 1-12 p.i. with peak levels of around seven times the day 0-levels. Apo A-I was decreased from days 1 to 8 and showed minimum levels of about 40% of day 0-levels around 1-2 days p.i. No clear pattern of changes in albumin levels could be identified. One pig, showing clinical signs on day 2 only, also showed an APP response. although of a relatively short duration, whereas three pigs presenting clinical signs for several days had a more protracted acute phase response. Remarkably, the one pig showing no clinical signs and no arthritic lesions showed an APP response comparable to that of the other, clinically affected pigs. Thus, both acute clinical and subclinical S. suis infection could be revealed by the measurement of one or more of the APPs CRP, SAA, Hp, pig-MAP and Apo A-I. The combined measurement of two or three APPs, including proteins with slow and fast kinetics, should be used to achieve the highest sensitivity for the detection of ongoing S. suis infection during a prolonged time period. A diagnostic tool based on such APP-measurements could considerably improve strategic control procedures for this important infection. (c) 2006 Elsevier B.V. All rights reserved.

    Original languageEnglish
    Pages (from-to)157-168
    Number of pages12
    JournalVeterinary Immunology and Immunopathology
    Volume113
    Issue number1-2
    Early online date13 Jun 2006
    DOIs
    Publication statusPublished - 15 Sep 2006

    Keywords

    • pig
    • Streptococcus suis
    • acute phase response
    • C-reactive protein
    • haptoglobin
    • pig major acute phase protein
    • serum amyloid A
    • albumin
    • apolipoprotein A-I
    • C-REACTIVE PROTEIN
    • SERUM HAPTOGLOBIN CONCENTRATION
    • ACTINOBACILLUS-PLEUROPNEUMONIAE
    • PIGS
    • TYPE-2
    • SWINE
    • PATHOGENICITY
    • CHALLENGE
    • Pig
    • Acute phase response
    • Haptoglobin
    • Pig major acute phase protein
    • Serum amyloid A
    • Albumin
    • Apolipoprotein A-I

    Cite this

    The porcine acute phase protein response to acute clinical and subclinical experimental infection with Streptococcus suis. / Sorensen, N. S.; Tegtmeier, C.; Andresen, L. O.; Pineiro, M.; Toussaint, M. J. M.; Campbell, Fiona Margaret; Lampreave, F.; Heegaard, P. M. H.

    In: Veterinary Immunology and Immunopathology, Vol. 113, No. 1-2, 15.09.2006, p. 157-168.

    Research output: Contribution to journalArticle

    Sorensen, N. S. ; Tegtmeier, C. ; Andresen, L. O. ; Pineiro, M. ; Toussaint, M. J. M. ; Campbell, Fiona Margaret ; Lampreave, F. ; Heegaard, P. M. H. / The porcine acute phase protein response to acute clinical and subclinical experimental infection with Streptococcus suis. In: Veterinary Immunology and Immunopathology. 2006 ; Vol. 113, No. 1-2. pp. 157-168.
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    T1 - The porcine acute phase protein response to acute clinical and subclinical experimental infection with Streptococcus suis

    AU - Sorensen, N. S.

    AU - Tegtmeier, C.

    AU - Andresen, L. O.

    AU - Pineiro, M.

    AU - Toussaint, M. J. M.

    AU - Campbell, Fiona Margaret

    AU - Lampreave, F.

    AU - Heegaard, P. M. H.

    PY - 2006/9/15

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    N2 - The pig acute phase protein (APP) response to experimental Streptococcus suis (S. suis) infection was mapped by the measurement of the positive APPs C-reactive protein (CRP), serum amyloid A (SAA), haptoglobin (Hp) and major acute phase protein (pig-MAP) and the negative APPs albumin and apolipoprotein (Apo) A-I. The aim was to elucidate the differences in the acute phase behaviour of the individual APPs during a typical bacterial septicaemic, infection. Pigs were inoculated subcutaneously with live S. suis serotype 2 and blood was sampled before and on various days post inoculation (p.i.), until the pigs were killed and autopsied on day 14 p.i. Clinical signs (fever and lameness) were observed in four of the five inoculated pigs from day 2 p.i., and these pigs also had arthritic lesions at autopsy. CRP and SAA showed fast increases in serum concentrations, CRP being elevated from days 1 to 12 p.i. and peaking at 10 times the day 0-levels on day 1 p.i. SAA rose quickly to peak levels of 30-40 times the day 0-level on days 1-2 and returned to pre-inoculation level on day 5 p.i. Hp and pig-MAP showed slightly slower responses, both peaking around 5 days p.i. Hp was increased throughout the experiment with maximum levels around 10 times the day 0-levels, and pig-MAP was elevated on days 1-12 p.i. with peak levels of around seven times the day 0-levels. Apo A-I was decreased from days 1 to 8 and showed minimum levels of about 40% of day 0-levels around 1-2 days p.i. No clear pattern of changes in albumin levels could be identified. One pig, showing clinical signs on day 2 only, also showed an APP response. although of a relatively short duration, whereas three pigs presenting clinical signs for several days had a more protracted acute phase response. Remarkably, the one pig showing no clinical signs and no arthritic lesions showed an APP response comparable to that of the other, clinically affected pigs. Thus, both acute clinical and subclinical S. suis infection could be revealed by the measurement of one or more of the APPs CRP, SAA, Hp, pig-MAP and Apo A-I. The combined measurement of two or three APPs, including proteins with slow and fast kinetics, should be used to achieve the highest sensitivity for the detection of ongoing S. suis infection during a prolonged time period. A diagnostic tool based on such APP-measurements could considerably improve strategic control procedures for this important infection. (c) 2006 Elsevier B.V. All rights reserved.

    AB - The pig acute phase protein (APP) response to experimental Streptococcus suis (S. suis) infection was mapped by the measurement of the positive APPs C-reactive protein (CRP), serum amyloid A (SAA), haptoglobin (Hp) and major acute phase protein (pig-MAP) and the negative APPs albumin and apolipoprotein (Apo) A-I. The aim was to elucidate the differences in the acute phase behaviour of the individual APPs during a typical bacterial septicaemic, infection. Pigs were inoculated subcutaneously with live S. suis serotype 2 and blood was sampled before and on various days post inoculation (p.i.), until the pigs were killed and autopsied on day 14 p.i. Clinical signs (fever and lameness) were observed in four of the five inoculated pigs from day 2 p.i., and these pigs also had arthritic lesions at autopsy. CRP and SAA showed fast increases in serum concentrations, CRP being elevated from days 1 to 12 p.i. and peaking at 10 times the day 0-levels on day 1 p.i. SAA rose quickly to peak levels of 30-40 times the day 0-level on days 1-2 and returned to pre-inoculation level on day 5 p.i. Hp and pig-MAP showed slightly slower responses, both peaking around 5 days p.i. Hp was increased throughout the experiment with maximum levels around 10 times the day 0-levels, and pig-MAP was elevated on days 1-12 p.i. with peak levels of around seven times the day 0-levels. Apo A-I was decreased from days 1 to 8 and showed minimum levels of about 40% of day 0-levels around 1-2 days p.i. No clear pattern of changes in albumin levels could be identified. One pig, showing clinical signs on day 2 only, also showed an APP response. although of a relatively short duration, whereas three pigs presenting clinical signs for several days had a more protracted acute phase response. Remarkably, the one pig showing no clinical signs and no arthritic lesions showed an APP response comparable to that of the other, clinically affected pigs. Thus, both acute clinical and subclinical S. suis infection could be revealed by the measurement of one or more of the APPs CRP, SAA, Hp, pig-MAP and Apo A-I. The combined measurement of two or three APPs, including proteins with slow and fast kinetics, should be used to achieve the highest sensitivity for the detection of ongoing S. suis infection during a prolonged time period. A diagnostic tool based on such APP-measurements could considerably improve strategic control procedures for this important infection. (c) 2006 Elsevier B.V. All rights reserved.

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    KW - Streptococcus suis

    KW - acute phase response

    KW - C-reactive protein

    KW - haptoglobin

    KW - pig major acute phase protein

    KW - serum amyloid A

    KW - albumin

    KW - apolipoprotein A-I

    KW - C-REACTIVE PROTEIN

    KW - SERUM HAPTOGLOBIN CONCENTRATION

    KW - ACTINOBACILLUS-PLEUROPNEUMONIAE

    KW - PIGS

    KW - TYPE-2

    KW - SWINE

    KW - PATHOGENICITY

    KW - CHALLENGE

    KW - Pig

    KW - Acute phase response

    KW - Haptoglobin

    KW - Pig major acute phase protein

    KW - Serum amyloid A

    KW - Albumin

    KW - Apolipoprotein A-I

    U2 - 10.1016/j.vetimm.2006.04.008

    DO - 10.1016/j.vetimm.2006.04.008

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    JO - Veterinary Immunology and Immunopathology

    JF - Veterinary Immunology and Immunopathology

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