The potential of Antheraea pernyi silk for spinal cord repair

A Varone; D Knight; S Lesage; F Vollrath; A M Rajnicek; W Huang

doi:10.1038/s41598-017-14280-5

The potential of Antheraea pernyi silk for spinal cord repair

A Varone, D Knight, S Lesage, F Vollrath, A M Rajnicek, W Huang

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Abstract

One of the most challenging applications for tissue regeneration is spinal cord damage. There is nocure for this, partly because cavities and scar tissue formed after injury present formidable barriersthat must be crossed by axons to restore function. Natural silks are considered increasingly formedical applications because they are biocompatible, biodegradable and in selected cases promotetissue growth. Filaments from wild Antheraea pernyi silkworms can support axon regeneration inperipheral nerve injury. Here we presented evidence that degummed A. pernyi filaments (DAPF)support excellent outgrowth of CNS neurons in vitro by cell attachment to high density of arginineglycine-aspartic acid tripeptide present in DAPF. Importantly, DAPF showed stiffness propertiesthat are well suited to spinal cord repair by supporting cell growth mechano-biology. Furthermore,we demonstrated that DAPF induced no activation of microglia, the CNS resident immune cells,either in vitro when exposed to DAPF or in vivo when DAPF were implanted in the cord. In vitroDAPF degraded gradually with a corresponding decrease in tensile properties. We conclude that A.pernyi silk meets the major biochemical and biomaterial criteria for spinal repair, and may havepotential as a key component in combinatorial strategies for spinal repair.

Original language	English
Article number	13790
Pages (from-to)	1-10
Number of pages	10
Journal	Scientific Reports
Volume	7
DOIs	https://doi.org/10.1038/s41598-017-14280-5
Publication status	Published - 23 Oct 2017

Keywords

biomaterials - cells
neurodegeneration

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10.1038/s41598-017-14280-5Licence: CC BY

The potential of Antheraea pernyi
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Final published version, 1.63 MBLicence: CC BY

Wenlong Huang
- School of Medicine, Medical Sciences & Nutrition, Medical Sciences - Senior Lecturer
- Institute of Medical Sciences
Person: Academic

Cite this

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title = "The potential of Antheraea pernyi silk for spinal cord repair",

abstract = "One of the most challenging applications for tissue regeneration is spinal cord damage. There is nocure for this, partly because cavities and scar tissue formed after injury present formidable barriersthat must be crossed by axons to restore function. Natural silks are considered increasingly formedical applications because they are biocompatible, biodegradable and in selected cases promotetissue growth. Filaments from wild Antheraea pernyi silkworms can support axon regeneration inperipheral nerve injury. Here we presented evidence that degummed A. pernyi filaments (DAPF)support excellent outgrowth of CNS neurons in vitro by cell attachment to high density of arginineglycine-aspartic acid tripeptide present in DAPF. Importantly, DAPF showed stiffness propertiesthat are well suited to spinal cord repair by supporting cell growth mechano-biology. Furthermore,we demonstrated that DAPF induced no activation of microglia, the CNS resident immune cells,either in vitro when exposed to DAPF or in vivo when DAPF were implanted in the cord. In vitroDAPF degraded gradually with a corresponding decrease in tensile properties. We conclude that A.pernyi silk meets the major biochemical and biomaterial criteria for spinal repair, and may havepotential as a key component in combinatorial strategies for spinal repair.",

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author = "A Varone and D Knight and S Lesage and F Vollrath and Rajnicek, {A M} and W Huang",

note = "This work was supported by the Institute of Medical Sciences of the University of Aberdeen, Scottish Rugby Union and RS McDonald Charitable Trust. We are grateful to Mr Nicholas Hawkins from Oxford University and Ms Annette Raffan from the University of Aberdeen for assistance with tensile testing. We thank Ms Michelle Gniβ for her help with the microglial response experiments. We also thank Mr Gianluca Limodio for assisting with the MATLAB script for automation of tensile testing{\textquoteright}s data analysis.",

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AU - Lesage, S

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AU - Rajnicek, A M

AU - Huang, W

N1 - This work was supported by the Institute of Medical Sciences of the University of Aberdeen, Scottish Rugby Union and RS McDonald Charitable Trust. We are grateful to Mr Nicholas Hawkins from Oxford University and Ms Annette Raffan from the University of Aberdeen for assistance with tensile testing. We thank Ms Michelle Gniβ for her help with the microglial response experiments. We also thank Mr Gianluca Limodio for assisting with the MATLAB script for automation of tensile testing’s data analysis.

PY - 2017/10/23

Y1 - 2017/10/23

N2 - One of the most challenging applications for tissue regeneration is spinal cord damage. There is nocure for this, partly because cavities and scar tissue formed after injury present formidable barriersthat must be crossed by axons to restore function. Natural silks are considered increasingly formedical applications because they are biocompatible, biodegradable and in selected cases promotetissue growth. Filaments from wild Antheraea pernyi silkworms can support axon regeneration inperipheral nerve injury. Here we presented evidence that degummed A. pernyi filaments (DAPF)support excellent outgrowth of CNS neurons in vitro by cell attachment to high density of arginineglycine-aspartic acid tripeptide present in DAPF. Importantly, DAPF showed stiffness propertiesthat are well suited to spinal cord repair by supporting cell growth mechano-biology. Furthermore,we demonstrated that DAPF induced no activation of microglia, the CNS resident immune cells,either in vitro when exposed to DAPF or in vivo when DAPF were implanted in the cord. In vitroDAPF degraded gradually with a corresponding decrease in tensile properties. We conclude that A.pernyi silk meets the major biochemical and biomaterial criteria for spinal repair, and may havepotential as a key component in combinatorial strategies for spinal repair.

AB - One of the most challenging applications for tissue regeneration is spinal cord damage. There is nocure for this, partly because cavities and scar tissue formed after injury present formidable barriersthat must be crossed by axons to restore function. Natural silks are considered increasingly formedical applications because they are biocompatible, biodegradable and in selected cases promotetissue growth. Filaments from wild Antheraea pernyi silkworms can support axon regeneration inperipheral nerve injury. Here we presented evidence that degummed A. pernyi filaments (DAPF)support excellent outgrowth of CNS neurons in vitro by cell attachment to high density of arginineglycine-aspartic acid tripeptide present in DAPF. Importantly, DAPF showed stiffness propertiesthat are well suited to spinal cord repair by supporting cell growth mechano-biology. Furthermore,we demonstrated that DAPF induced no activation of microglia, the CNS resident immune cells,either in vitro when exposed to DAPF or in vivo when DAPF were implanted in the cord. In vitroDAPF degraded gradually with a corresponding decrease in tensile properties. We conclude that A.pernyi silk meets the major biochemical and biomaterial criteria for spinal repair, and may havepotential as a key component in combinatorial strategies for spinal repair.

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KW - neurodegeneration

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DO - 10.1038/s41598-017-14280-5

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JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

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ER -

The potential of Antheraea pernyi silk for spinal cord repair

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